7 research outputs found

    Preferential orientation of diamond formation on TaC: Diamond(111)//TaC(111)

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    Experimental results showed that for the diamond film prepared by hot filament chemical vapor deposition (HFCVD) using Ta filament, TaC existed between diamond and the silicon substrate, and diamond grew directly on TaC, while the inherent mechanism was not clear. Here, a special coherent interface Diamond(111)//TaC(111) is observed using high resolution transmission electron microscopy, and then we explore the effects of the TaC with different lattice planes on the diamond formation by first-principle calculations. The results show that C tends to adsorb on the TaC(111) C-terminated surface. The strong covalent bond between C from diamond and Ta from TaC is formed in the Diamond(111)//TaC(111) interface, while only C–C covalent bonds are formed at the Graphite(002)/TaC(111). This makes diamond thermodynamically more stable than graphite on the TaC surfaces. Our investigations provide critical information to understand the complex diamond formation mechanism, especially with the presence of TaC.</p

    Expression of ITPKB protein in 3 lung carcinoma subtypes.

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    <p>Western blot was used to detect the expression of ITPKB protein in lung carcinoma tissues (2AC samples, 2SQ samples, 3SCLC samples) and adjacent normal lung tissue. The ITPKB protein intensities were normalized by GAPDH.The fold changes were 0.2and0.3 in the comparisons of 2AC vs. normal, 0.2 and 0.4of 2SQ vs. normal, 0.3, 0.3 and 0.4of 3SCLC vs. normal, respectively.</p

    Correlation of miR-375 with target mRNA expression.

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    <p><b>A,</b> Correlation of miR-375 with target mRNA expression in the comparison of AC vs. normal. <b>B,</b> Correlation of miR-375 with target mRNA expression in the comparison of SQ vs. normal. <b>C,</b> Correlation of miR-375 with target mRNA expression in the comparison of SCLC vs. normal. Green circle: down-regulation. Red circle: up-regulation. White circle: the gene did not show differential expression in the pair-wise comparison of AC vs. normal, SQ vs. normal or SCLC vs. normal. Blue circle: the gene did not pass the quality control. AC: adenocarcinoma, SQ: squamous cell carcinoma, SCLC: small cell lung carcinoma.</p

    Effect of miR-375 on cell growth of NCI-H82.

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    <p><b>A,</b> miR-375 expression of both H82-NC and H82-miR-375.NCI-H82 cells were infected with miR-375-expressing (H82-miR-375) or empty (H82-NC) lentivirus prior to the proliferation assay. <b>B,</b> miR-375 could promote cell growth of H82 cells. The infected cells were seeded to 96-wells plates. After incubation for several days, the cell growth was measured by CCK-8 assay. *: p< 0.01, compared with H82-NC.</p

    Expression profiles of miR-375 in 3 lung carcinoma subtypes.

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    <p><b>A</b>, Hierarchical clustering of miRNA expression profiles in AC, SQ, SCLC and normal lung tissue on microarrays. The mean signal from biological replicate samples was used for the clustering. Colored bars indicate the range of normalized log2-based signals. <b>B</b>, An interactive dot diagram of miR-375 normalized signals on microarrays. <b>C</b>, An interactive dot diagram of miR-375 normalized CT values on qRT-PCR. AC: adenocarcinoma, SQ: squamous cell carcinoma, SCLC: small cell lung carcinoma.</p
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