DataSheet_1_Response of Photosynthesis to High Growth Temperature Was Not Related to Leaf Anatomy Plasticity in Rice (Oryza sativa L.).docx

Photosynthesis is highly sensitive to high temperature stress, and with the rising global temperature, it is meaningful to investigate the response of photosynthesis to growth temperature and its relationship with leaf anatomy plasticity. We planted 21 cultivars including eight indica cultivars, eight japonica cultivars, and five javanica cultivars in pot experiments under high growth temperature (HT, 38/28°C, day/night) and control treatment (CK, 30/28°C, day/night). Photosynthetic rate (A), stomatal conductance (gs), transpiration rate (E), stomatal density (SD), vein density (VD), minor vein area (SVA), and major vein area (LVA) were measured after 30 treatment days. Results showed HT significantly increased A, gs, and E, while significantly decreased SD and LVA. There was no significant difference in A among the three subspecies both under CK and HT, while the javanica subspecies had higher gs, E, SVA, and LVA under HT, and the indica cultivars had higher VD and SD both under CK and HT. The javanica subspecies had higher relative value (HT/CK) of A, gs, and E, while difference was not observed in the relative value of SD, VD, and LVA among the three subspecies. The relative value of A was positively related to that of gs, while the latter was not correlated with the relative value of SD, VD, SVA, and LVA. Overall, the results suggested the increase of A and gs at HT was not attributed to leaf anatomy plasticity in respect of stomata and vein under HT.</p

The leaf width, thickness, and specific leaf weight (SLW) of the wide and narrow sides of leaf blade in the top three leaves at heading stage.

<p>The experiments were conducted in the early, middle, and late growing seasons in Wuxue County and Wuhan City, Hubei Province, China in 2014.</p><p>Within a row for each leaf position, means followed by different letters are significantly different according to the least significant difference (LSD) at the 0.05 probability level.</p><p>The leaf width, thickness, and specific leaf weight (SLW) of the wide and narrow sides of leaf blade in the top three leaves at heading stage.</p

Leaf nitrogen concentration based on dry weight (Nw) and leaf area (Na), and chlorophyll meter reading (SPAD) of the wide and narrow sides of leaf blade in the top three leaves at heading stage.

<p>The experiments were conducted in the early, middle, and late growing seasons in Wuxue County and Wuhan City, Hubei Province, China in 2014.</p><p>Within a row for each leaf position, means followed by different letters are significantly different according to the least significant difference (LSD) at the 0.05 probability level.</p><p>Leaf nitrogen concentration based on dry weight (Nw) and leaf area (Na), and chlorophyll meter reading (SPAD) of the wide and narrow sides of leaf blade in the top three leaves at heading stage.</p

Correlation between the wide and narrow sides of leaf blade in specific leaf weight (SLW).

<p>(A) The flag, (B) The -2^nd, and (C) The -3^rd leaves at heading stage. Each data point represents the mean of the four replications. Data (n = 40) were from the four field experiments conducted in three growing seasons in two locations in 2014.</p

Correlation between the wide and narrow sides of leaf blade in leaf thickness.

<p>(A) The flag, (B) The -2^nd, and (C) The -3^rd leaves at heading stage. Each data point represents the mean of the three plants and four replications. Data (n = 40) were from the four field experiments conducted in three growing seasons in two locations in 2014.</p

General information on the rice varieties grown in the early (April to July), middle (May to October), and late (July to November) growing seasons in Wuxue County and Wuhan City, Hubei Province, China in 2014.

<p>General information on the rice varieties grown in the early (April to July), middle (May to October), and late (July to November) growing seasons in Wuxue County and Wuhan City, Hubei Province, China in 2014.</p

Correlation between the wide and narrow sides in dry weight-based nitrogen concentration (Nw).

<p>(A) The flag, (B) The -2^nd, and (C) The -3^rd leaves at heading stage. Each data point represents the mean of the four replications. Data (n = 40) were from the four field experiments conducted in three growing seasons in two locations in 2014.</p

Correlation between the wide and narrow sides in leaf area-based nitrogen concentration (Na).

<p>(A) The flag, (B) The -2^nd, and (C) The -3^rd leaves at heading stage. Each data point represents the mean of the four replications. Data (n = 40) were from the four field experiments conducted in three growing seasons in two locations in 2014.</p

Correlation between the wide and narrow sides of leaf blade in chlorophyll meter reading (SPAD).

<p>(A) The flag, (B) The -2^nd, and (C) The -3^rd leaves at heading stage. Each data point represents the mean of the three plants and four replications. Data (n = 40) were from the four field experiments conducted in three growing seasons in two locations in 2014.</p

Correlation between the wide and narrow sides of leaf blade in leaf width.

<p>(A) The flag, (B) The -2^nd, and (C) The -3^rd leaves at heading stage. Each data point represents the mean of the three plants and four replications. Data (n = 40) were from the four field experiments conducted in three growing seasons in two locations in 2014.</p