第828回千葉医学会例会・第6回磯野外科例会 88-3.

Western blotting was performed to examine the protein levels of Twist in the indicated cells; β-actin was used as control. (JPG 151 kb

Commissioning and integration testing of the DAQ system for the CMS GEM upgrade

The CMS muon system will undergo a series of upgrades in the coming years to preserve and extend its muon detection capabilities during the High Luminosity LHC.The first of these will be the installation of triple-foil GEM detectors in the CMS forward region with the goal of maintaining trigger rates and preserving good muon reconstruction, even in the expected harsh environment.In 2017 the CMS GEM project is looking to achieve a major milestone in the project with the installation of 5 super-chambers in CMS; this exercise will allow for the study of services installation and commissioning, and integration with the rest of the subsystems for the first time. An overview of the DAQ system will be given with emphasis on the usage during chamber quality control testing, commissioning in CMS, and integration with the central CMS system.The international CMS collaboration at the CERN-LHC experiment will conduct a major upgrade to its muon detection system to cope with the intense particle flux and radiation levels foreseen during the high-luminosity LHC era. The first CMS approved muon upgrade project (GE1/1) consists on the installation of new detectors in the CMS forward region based on Gas Electron Multiplier (GEM) technology to maintain an excellent muon reconstruction and particle identification in that region. Extensive simulation and test-beam exercises performed during the past few years ensure the optimal detector operation. Integration of five GEM modules into CMS done early 2017 allowed scientists to gain experience on mechanical installation, services and data acquisition (DAQ) infrastructures. This letter presents an overall description of the project with emphasis on the GEM data acquisition (GEM-DAQ) system and results on GEM local calibrations with the detector integrated into CMS for the first time

Effects of simulated drought stress on early seedling growth of <i>Nyssa yunnanensis</i>.

<p>Effects of simulated drought stress on early seedling growth of <i>Nyssa yunnanensis</i>.</p

Significance level of the effects of factors and factor interactions on variables based on repeated-measure one-way ANOVA.

<p>Significance level of the effects of factors and factor interactions on variables based on repeated-measure one-way ANOVA.</p

Effects of water condition on the growth parameters of <i>Nyssa yunnanensis</i> seedlings.

<p>Effects of water condition on the growth parameters of <i>Nyssa yunnanensis</i> seedlings.</p

Exonuclease-Catalyzed Methylene Blue Releasing and Enriching onto a Dodecanethiol Monolayer for an Immobilization-Free and Highly Sensitive Electrochemical Nucleic Acid Biosensor

Herein, a unique and versatile immobilization-free electrochemical nucleic acid biosensor architecture is proposed for the first time based on the catalyzed release of a methylene blue (MB)-tagged mononucleotide by exonuclease III (Exo III) and the successive enrichment onto a dodecanethiol monolayer, which can be attributed to the hydrophobic force between the alkyl chain of the dodecanethiol monolayer and the hydrophobic part of the MB-tagged mononucleotide. The fabricated biosensor demonstrates considerable advantages including assay simplicity, rapidness, and high sensitivity owing to its immobilization-free and homogenous operation for the biorecognition and amplification process. A low detection limit of approximately 1 pM toward the target DNA could be achieved with an excellent selectivity. The proposed immobilization-free electrochemical biosensing strategy was also extended for the assay of Exo I and III activity. Furthermore, it might be easily extended for the detection of a wide spectrum of targets and thus provide a promising avenue for the development of immobilization-free and sensitive electrochemical biosensors

Design and Synthesis of Nonionic Copolypeptide Hydrogels with Reversible Thermoresponsive and Tunable Physical Properties

Polypeptide-based formulations that undergo liquid to hydrogel transitions upon change in temperature have become desirable targets since they can be mixed with cells or injected into tissues as liquids, and subsequently transform into rigid scaffolds or depots. Such materials have been challenging to prepare using synthetic polypeptides, especially when reversible gelation and tunable physical properties are desired. Here, we designed and prepared new nonionic diblock copolypeptide hydrogels (DCH) containing hydrophilic poly­(γ-[2-(2-methoxyethoxy)­ethyl]-<i>rac</i>-glutamate) and hydrophobic poly­(l-leucine) segments, named DCH_EO, and also further incorporated copolypeptide domains into DCH_EO to yield unprecedented thermoresponsive DCH, named DCH_T. Although previous attempts to prepare nonionic hydrogels composed solely of synthetic polypeptides have been unsuccessful, our designs yielded materials with highly reversible thermal transitions and tunable properties. Nonionic, thermoresponsive DCH_T were found to support the viability of suspended mesenchymal stem cells in vitro and were able to dissolve and provide prolonged release of both hydrophilic and hydrophobic molecules. The versatility of these materials was further demonstrated by the independent molecular tuning of DCH_T liquid viscosity at room temperature and DCH_T hydrogel stiffness at elevated temperature, as well as the DCH_T liquid to hydrogel transition temperature itself

Prevalence of hVISA and VISA based on study period, origin of study, and isolate selection.^a

<p>CI, confidence interval</p><p>^a References: [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0136082#pone.0136082.ref018" target="_blank">18</a>–<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0136082#pone.0136082.ref108" target="_blank">108</a>].</p><p>Prevalence of hVISA and VISA based on study period, origin of study, and isolate selection.<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0136082#t002fn002" target="_blank">^a</a></p

Flowchart of study selection.

<p>Flowchart of study selection.</p

Genetic prevalence of hVISA and VISA.^a

<p>CI, confidence interval</p><p>^aReferences: [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0136082#pone.0136082.ref021" target="_blank">21</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0136082#pone.0136082.ref026" target="_blank">26</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0136082#pone.0136082.ref028" target="_blank">28</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0136082#pone.0136082.ref029" target="_blank">29</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0136082#pone.0136082.ref033" target="_blank">33</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0136082#pone.0136082.ref044" target="_blank">44</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0136082#pone.0136082.ref046" target="_blank">46</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0136082#pone.0136082.ref052" target="_blank">52</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0136082#pone.0136082.ref055" target="_blank">55</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0136082#pone.0136082.ref059" target="_blank">59</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0136082#pone.0136082.ref065" target="_blank">65</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0136082#pone.0136082.ref070" target="_blank">70</a>–<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0136082#pone.0136082.ref072" target="_blank">72</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0136082#pone.0136082.ref074" target="_blank">74</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0136082#pone.0136082.ref076" target="_blank">76</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0136082#pone.0136082.ref083" target="_blank">83</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0136082#pone.0136082.ref084" target="_blank">84</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0136082#pone.0136082.ref086" target="_blank">86</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0136082#pone.0136082.ref092" target="_blank">92</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0136082#pone.0136082.ref105" target="_blank">105</a>]</p><p>Genetic prevalence of hVISA and VISA.<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0136082#t003fn002" target="_blank">^a</a></p