Functional classification and associated mapped reads deriving from the CDS associated with putative secreted proteins found in the sialotranscriptome of <i>Amblyomma americanum</i>.

<p>Functional classification and associated mapped reads deriving from the CDS associated with putative secreted proteins found in the sialotranscriptome of <i>Amblyomma americanum</i>.</p

Heat map displaying 98 Kunitz domain containing coding sequences (CDS) that are not uniformly expressed in the four libraries and showing a standard deviation of 1 or more regarding the log(10) transformation of the average normalized data.

<p>For more details, see text.</p

Heat map displaying 138 lipocalin coding sequences (CDS) that are not uniformly expressed in the four libraries and showing a standard deviation of 1 or more regarding the log(10) transformation of the average normalized data.

<p>For more details, see text.</p

Time-invariant expressed transcripts.

<p>FPKM were average-transformed (divided by the row average).</p><p>Time-invariant expressed transcripts.</p

Functional classification and associated mapped reads deriving from the sialotranscriptome of <i>Amblyomma americanum</i>.

<p>Functional classification and associated mapped reads deriving from the sialotranscriptome of <i>Amblyomma americanum</i>.</p

Additional file 1: of Rickettsia parkeri colonization in Amblyomma maculatum: the role of superoxide dismutases

Figure S1.  Evolutionary relationships of taxa based on the SOD amino acid sequence using maximum likelihood method. The evolutionary history was inferred by using the Maximum Likelihood method based on the JTT matrix-based model [15]. The tree is drawn to scale, with the branch lengths measured by the number of substitutions per site. Evolutionary analyses were conducted using MEGA6 software [14]. The sequences were obtained from Amblyomma maculatum, Amblyomma variegatum, Ixodes scapularis, Anopheles gambiae, Mus musculus, Sus scrofa, and Homo sapiens. GenBank accession numbers followed by species names are shown in the tree. Figure S2A. Multiple sequence alignments of Cu/Zn-SOD amino acid sequences from different taxa. Regions outlined by red boxes indicate metal-binding sites that are conserved between all of the listed species. The sequences for Cu/Zn-SODs were obtained from Drosophila melanogaster, Apis mellifera, Amblyomma maculatum, Thermostable mutant of Human Cu/Zn SOD, Mus musculus, Equus caballus, Danio rerio, Anopheles gambiae and Culex quinquefasciatus. Figure S2B. Multiple sequence alignments of Mn-SOD amino acid sequences from different taxa. Regions outlined by red boxes indicate metal-binding sites that are conserved between all the listed species. The sequences were obtained from Haliotis discus, Lottia gigantea, Perinereis nuntia, Stegastes partitus, Takifugu rubripes, Struthio camelus, Tauraco erythrolophus, Thamnophis elegans, Sus scrofa, Macaca mulatta, Mus musculus, Xenopus laevis, Amblyomma variegatum, Amblyomma maculatum and Ixodes scapularis. Figure S3. The engorged tick weight in dsRNA-SODs injected ticks. The engorged weights of ticks injected with dsLacZ, dsCu/Zn-SOD, dsMn-SOD and dual SODs (dsCu/Zn-SOD and dsMn-SOD) observed at the detachment of ticks from Sheep. There were no significant effects in tick engorged weights with dsRNA-SODs (ANOVA, F (3,52) = 0.6274, P = 0.6006). (DOCX 473 kb

Clustering of the differential temporal expression of 817 transcripts from the <i>Amblyomma americanum</i> sialotranscriptome, using the Click algorithm of the Expander program [24].

<p>Nineteen transcripts of the 836 shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0131292#pone.0131292.g001" target="_blank">Fig 1</a> were not clustered. Each graph (A-E) represents an identified cluster. The Y axis represent the log(10) of the normalized FPKM row data, where 0 reads were substituted by 1. Symbols and bars represent the average and standard errors. For more details, see text.</p

Functional classification of coding sequences and their relative abundance in the 5,792 set (A) or in the differentially expressed set of 836 sequences (B).

<p>Functional classification of coding sequences and their relative abundance in the 5,792 set (A) or in the differentially expressed set of 836 sequences (B).</p

Temporal expression of housekeeping genes.

<p>Raw Ct values for the mRNA of seven <i>A</i>. <i>americanum</i> housekeeping genes from tick salivary glands (A) and midgut (B) throughout a blood meal (unfed–168 h). Each gene was amplified from each sample and the point at which the amplification curve crosses the threshold is considered the Ct.</p

Temporal expression of seven tick-specific genes with unknown functions.

<p>AamerSigp-22563 was not expressed, according to qRT–PCR.</p