MOESM4 of Heterologous synthesis of chlorophyll b in Nannochloropsis salina enhances growth and lipid production by increasing photosynthetic efficiency

Additional file 4: Figure S3. RESDA PCR of WT and NsChlb transformants using DegTaqI as a degenerate primer

MOESM2 of Heterologous synthesis of chlorophyll b in Nannochloropsis salina enhances growth and lipid production by increasing photosynthetic efficiency

Additional file 2: Table S1. Primers used in this study

MOESM5 of Heterologous synthesis of chlorophyll b in Nannochloropsis salina enhances growth and lipid production by increasing photosynthetic efficiency

Additional file 5: Figure S4. Localization of CrCAO in a transformant (NsChlb19) examined with immuno-gold labelling and transmission electron microscopy (TEM)

MOESM1 of Heterologous synthesis of chlorophyll b in Nannochloropsis salina enhances growth and lipid production by increasing photosynthetic efficiency

Additional file 1: Figure S1. Sequences of signal and transit peptides from GCSL in N. salina and coding sequence of CrCAO

MOESM3 of Heterologous synthesis of chlorophyll b in Nannochloropsis salina enhances growth and lipid production by increasing photosynthetic efficiency

Additional file 3: Figure S2. The prediction of leader sequence and localization by computational methods

Additional file 3 of Development and characterization of a Nannochloropsis mutant with simultaneously enhanced growth and lipid production

Additional file 3: Table S2. Dry cell weight and biomass productivity analysis of WT and Mut68

Additional file 1 of Development and characterization of a Nannochloropsis mutant with simultaneously enhanced growth and lipid production

Additional file 1: Table S1. Oligonucleotides used in this study

Additional file 7 of Development and characterization of a Nannochloropsis mutant with simultaneously enhanced growth and lipid production

Additional file 7: Fig. S4. Predicted binding pocket in WT TPP domain. The Mg2+ ion binding site of WTTPP domain was expressed

Additional file 2 of Development and characterization of a Nannochloropsis mutant with simultaneously enhanced growth and lipid production

Additional file 2: Fig. S1. Whole gel Southern blot result. Lane 1, WT (no digestion); lane 2, Mut68 (no digestion); Lane 3, WT (digested by NheI); lane 4, Mut68 (digested by NheI); Lane 5, WT (digested by NheI and XhoI); lane 6, Mut68 (digested by NheI and XhoI)

Additional file 6 of Development and characterization of a Nannochloropsis mutant with simultaneously enhanced growth and lipid production

Additional file 6: Fig. S3. Predicted protein crystal structure of WT (a) and truncated (b) TPS based on the template-based prediction tool RaptorX. Yellow, TPSWT and TPS Tr domain; cyan, TPPWT and TPPTr domain; purple, N-terminalWT and N-terminalTr domain; orange, C-terminalWT; grey, disordered region