Media 2: Fibre-coupled multiphoton microscope with adaptive motion compensation

Originally published in Biomedical Optics Express on 01 May 2015 (boe-6-5-1876

Media 1: Fibre-coupled multiphoton microscope with adaptive motion compensation

Originally published in Biomedical Optics Express on 01 May 2015 (boe-6-5-1876

Affective design (kansei engineering) in Japan : a report from a DTI International Technology Service Mission

New products introduced by consumer goods companies operating in many market sectors are often not as successful as expected even though they are functionally reliable and produced to a consistent standard. They do not seem to connect with the feelings of customers who are rejecting anything that does not truly satisfy them in the way that they want to live. The Japanese realised earlier than most that their product development methods just did not reveal the deep insights into customers’ feelings that enabled emotional needs to be satisfied. They have developed methods and tools to overcome this, supporting the creation of products targeted to meet those needs, that they call Kansei Engineering. This report is about a mission to Japan to study that. Experience of the use of Kansei Engineering was sought from the food and drink (Asahi Breweries Ltd.), packaging (Toppan Printing Co. Ltd.), building products (Matsushita Electric Works Ltd.), cosmetics (Shiseido Company Ltd., Milbon Co., Ltd.), electronic products (Seiko Epson Corporation) and automotive (Mazda Motor Corporation) sectors, and from three Universities (Hiroshima International, Shinshu and Tsukuba)

Multispectral fluorescence intensity and FLIM images acquired from normal skin and BCCs.

<p>(a–i) Fluorescence intensity and false color FLIM images from a single field of view acquired at a depth of 110 µm with all spectral channels taken near a dermal papilla from normal skin. (j–n) FLIM images taken from the green channel only of different depths within a sample of normal skin. (o–u) FLIM images taken from the green channel illustrating visual architectural features seen in BCC using MPT. (v) FLIM image taken from the blue channel of a BCC. (w,x) paired FLIM images taken from the green and blue channels respectively of a BCC nest. KEY SG-Stratum Granulosum, SS- Stratum Spinosum, BCL-Basal Cell Layer, DP-Dermal Papilla. Scale bar 25 µm.</p

Exemplar segmented fluorescence intensity images, fitted fluorescence decay and fluorophore emission spectra.

<p>(a) Total fluorescence intensity image, same image with (b) manually and (c) automatically defined cellular regions of interest overlayed. (d) Top – exemplar fluorescence decay from one region of interest (black), biexponential fit to data (green) and instrument response function (blue). (d) Bottom – normalized residuals. (e) The emission spectra from endogenous fluorophores plotted in relation to the four spectral detection channels.</p

Patient characteristics and median lifetimes for each spectral channel.

<p><b>Abbreviations</b> ROI-region of interest, τ_mean - mean fluorescence lifetime, ps - picoseconds.</p

The best discriminatory parameters ranked using the area under the curve (AUC).

<p><b>Abbreviations</b> AUC – area under the curve, τ₁ – short fluorescence lifetime decay component, τ₂ – long lifetime component.</p