Ethical Statement;Material and methods;Suppl Tables;Suppl Figure from Inverted intergeneric introgression between critically endangered kipunjis and yellow baboons in two disjunct populations

Additional information on Ethical Statement;Additional information on Material and methods. Sample Collection; Laboratory methods; Phylogenetic analysis; References;Suppl. Table 1 List of samples. ID, clade, geographic provenance and accession numbers; Suppl. Table 2. Estimated divergence ages of mtDNA lineages in million years (Ma); Suppl. Table 3. Results from the Kishino-Hasegawa (KH) and Shimodaira-Hasegawa (SH) tests;Suppl Fig 1 is showing an uncollapsed chronogram showing phylogenetic relationships and divergence times among various baboon and kipunji mtDNA lineage

Performance characteristics of the serological tests used in this study, as reported by the manufacturer. Sen = Sensitivity, Spec = Specificity, n.p. = not provided.

<p>Performance characteristics of the serological tests used in this study, as reported by the manufacturer. Sen = Sensitivity, Spec = Specificity, n.p. = not provided.</p

Multiple comparisons of log anti-<i>T</i>. <i>pallidum</i> titers in 4 groups with a different stage of genital ulceration in baboons

<p>([CNA] = clinically non-affected (n = 20), [INI] = initial (n = 14), [MOD] = moderate (n = 7), and [SEV] = severe genital ulceration (n = 16); for stage definition see [<a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0003637#pntd.0003637.ref018" target="_blank">18</a>]), GU = genital ulceration. Anti-<i>T</i>. <i>pallidum</i> antibody quantification was investigated using the Serodia TP-PA. Kruskal-Wallis test using Dunn’s correction for multiple comparison: CNA vs. SEV mean rank diff. = -30.04, p ≤ 0.0001; CNA vs. MOD mean rank diff. = -19.95, p ≤ 0.05; INI vs. SEV mean rank diff. = -17.56, p ≤ 0.05. (mean ± SEM).</p

Comparison of the serological tests with the consensus of infection status (Table 2).

<p>Comparison of the serological tests with the consensus of infection status (<a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0003637#pntd.0003637.t002" target="_blank">Table 2</a>).</p

Definitions used to determine the infectious stage of baboons.

<p>Definitions used to determine the infectious stage of baboons.</p

<i>T</i>. <i>pallidum</i> test algorithm for the screening of wild non-treated baboons.

<p>Based on the test performances Espline TP is recommended as the initial screening test followed by a confirmatory test e.g. Mastablot TP IgG that has been identified as most reliable standard. Dashed lines indicate reported results, while continuous lines represent the workflow.</p

Comparison of treponemal serological tests with the results of the Serodia TP-PA.

<p>Comparison of treponemal serological tests with the results of the Serodia TP-PA.</p

Detection limits of LAMP assays.

<p>(A-C) <i>TP</i> LAMP assay involving (A) <i>TPA</i> strain Nichols with copy numbers 10E+5 to 10E+0, (B) <i>TPE</i> strain Gauthier tested in a range of 10E+6 to 10E+0 copies, and (C) <i>TEN</i> strain Bosnia A in 10E+6 to 10E+0 copy numbers. (D-E) <i>TPA</i> LAMP assay run with <i>TPA</i> strain Nichols from 10E+5 to 10E+0 copy numbers, and the same LAMP assay run with (E) <i>TEN</i> strain Bosnia A in copy numbers ranging from 10E+6 to 10E+0. (F-G) <i>TPE</i>/<i>TEN</i> LAMP assay involving (E) <i>TPE</i> strain Gauthier tested in 10E+6 to 10E+0 copy numbers, and (F) <i>TEN</i> strain Bosnia A tested in 10E+6 to 10E+0 copies. Red cross = Negative control, <i>TPA</i> strain Nichols (blue), <i>TPE</i> strain Gauthier (green), <i>TEN</i> strain Bosnia A (black); symbols represent copy numbers: circle with cross = 10E+6, hexagon = 10E+5, square turned = 10E+4, square = 10E+3, circle = 10E+2, down-pointing triangle = 10E+1, up-pointing triangle = 10E+0.</p

Additional file 1: Table S1. of Insights into the genetic foundation of aggression in Papio and the evolution of two length-polymorphisms in the promoter regions of serotonin-related genes (5-HTTLPR and MAOALPR) in Papionini

Sample locations and genotypes. (PDF 37 kb

Detection limits for the three LAMP assays.

<p>Corresponding graphs can be found in <a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0006396#pntd.0006396.g002" target="_blank">Fig 2</a>. + = exponential amplification, (+) = no exponential amplification,— = no amplification.</p