Isolation from <i>Eucalyptus </i><i>o</i><i>ccidentalis</i> and Identification of a New Kaempferol Derivative that Induces Apoptosis in Human Myeloid Leukemia Cells

In this paper we report the isolation and structural elucidation of a new flavonoid (1) and three known compounds, 6,8-di-C-methylkaempferol 3-methyl ether (2), oleanolic acid, and 2α,3β-dihydroxyurs-12-en-28-oic acid, from aerial parts of Eucalyptus occidentalis collected in Algeria. Flavonoids 1 and 2 were used to study their biological activities on the human promyelocytic leukemia cell line, HL-60. Our data show that these compounds induce morphological changes and internucleosomal DNA fragmentation characteristic of apoptotic cell death, which is mediated by caspase-8/caspase-3 activation and cytochrome c release

Isolation from <i>Eucalyptus </i><i>o</i><i>ccidentalis</i> and Identification of a New Kaempferol Derivative that Induces Apoptosis in Human Myeloid Leukemia Cells

In this paper we report the isolation and structural elucidation of a new flavonoid (1) and three known compounds, 6,8-di-C-methylkaempferol 3-methyl ether (2), oleanolic acid, and 2α,3β-dihydroxyurs-12-en-28-oic acid, from aerial parts of Eucalyptus occidentalis collected in Algeria. Flavonoids 1 and 2 were used to study their biological activities on the human promyelocytic leukemia cell line, HL-60. Our data show that these compounds induce morphological changes and internucleosomal DNA fragmentation characteristic of apoptotic cell death, which is mediated by caspase-8/caspase-3 activation and cytochrome c release

Isolation from <i>Eucalyptus </i><i>o</i><i>ccidentalis</i> and Identification of a New Kaempferol Derivative that Induces Apoptosis in Human Myeloid Leukemia Cells

In this paper we report the isolation and structural elucidation of a new flavonoid (1) and three known compounds, 6,8-di-C-methylkaempferol 3-methyl ether (2), oleanolic acid, and 2α,3β-dihydroxyurs-12-en-28-oic acid, from aerial parts of Eucalyptus occidentalis collected in Algeria. Flavonoids 1 and 2 were used to study their biological activities on the human promyelocytic leukemia cell line, HL-60. Our data show that these compounds induce morphological changes and internucleosomal DNA fragmentation characteristic of apoptotic cell death, which is mediated by caspase-8/caspase-3 activation and cytochrome c release

Isolation from <i>Eucalyptus </i><i>o</i><i>ccidentalis</i> and Identification of a New Kaempferol Derivative that Induces Apoptosis in Human Myeloid Leukemia Cells

In this paper we report the isolation and structural elucidation of a new flavonoid (1) and three known compounds, 6,8-di-C-methylkaempferol 3-methyl ether (2), oleanolic acid, and 2α,3β-dihydroxyurs-12-en-28-oic acid, from aerial parts of Eucalyptus occidentalis collected in Algeria. Flavonoids 1 and 2 were used to study their biological activities on the human promyelocytic leukemia cell line, HL-60. Our data show that these compounds induce morphological changes and internucleosomal DNA fragmentation characteristic of apoptotic cell death, which is mediated by caspase-8/caspase-3 activation and cytochrome c release

Isolation from <i>Eucalyptus </i><i>o</i><i>ccidentalis</i> and Identification of a New Kaempferol Derivative that Induces Apoptosis in Human Myeloid Leukemia Cells

In this paper we report the isolation and structural elucidation of a new flavonoid (1) and three known compounds, 6,8-di-C-methylkaempferol 3-methyl ether (2), oleanolic acid, and 2α,3β-dihydroxyurs-12-en-28-oic acid, from aerial parts of Eucalyptus occidentalis collected in Algeria. Flavonoids 1 and 2 were used to study their biological activities on the human promyelocytic leukemia cell line, HL-60. Our data show that these compounds induce morphological changes and internucleosomal DNA fragmentation characteristic of apoptotic cell death, which is mediated by caspase-8/caspase-3 activation and cytochrome c release

Phytochemical analysis, antioxidant and hypoglycemic activities of a methanol extract from <i>Stachys brachyclada</i> de Noé ex Coss. leaves

Stachys brachyclada de Noé ex Coss. (Lamiaceae) is a quite rare medicinal plant endemic to the Mediterranean basin. In this study, seven secondary metabolites from a methanol extract of its leaves have been isolated and identified by a combination of chromatographic and spectroscopic methods (1D and 2D NMR experiments and ESIMS analysis). They include one ethyl 4-hydroxybenzoate (1), three acylated flavone glycosides (2–4), one diapigenin derivative (5) and two flavone aglycones (6–7). Stachysetin (5) was found the major compound of the extract (74.0 mg/g of dry matter). Moreover, the produced extract showed the ability in inhibiting the α-glucosidase enzyme (IC50 = 13.7 µg/mL), in quenching the radical 1,1-diphenyl-2-picrylhydrazyl (EC50 = 74.6 µg/mL), and in reducing the intracellular oxidative stress level in Human Dermal Fibroblast (64% inhibition at 50 µg/mL).</p

Exploring the Bioactive Terpenoid Content of an Algerian Plant of the Genus <i>Pulicaria</i>: The <i>ent</i>-Series of Asteriscunolides

Chemical analysis of the chloroform extract of the aerial parts of the Algerian plant <i>Pulicaria undulata</i> exhibiting anti-inflammatory activity resulted in the isolation of 10 new humulene sesquiterpenoids, <b>1</b>–<b>10</b>, belonging to the asteriscunolide family of compounds. The structure and relative configuration have been defined by NMR data, whereas the absolute configuration has been established by ECD analysis. Compounds <b>1</b>–<b>10</b> include enantiomers of the known asteriscunolides A–D and tetrahydroasteriscunolide previously reported from the genera <i>Asteriscus</i> and <i>Nauplius</i>. Compounds <b>1</b> and <b>10</b> showed in vitro anti-inflammatory activity by inhibiting heat-induced albumin denaturation with IC₅₀ values of 23.76 and 220.42 μM