Kimpul (Xanthosoma spp.) characterization based on morphological characteristic and isozymic analysis

Nurmiyati, Sugiyarto, Sajidan. 2009. Kimpul (Xanthosoma spp.) characterization based on morphological characteristic and isozymic analysis. Nusantara Bioscience 1: 138-145. This research is aimed: (i) to know the variety of kimpul (Xanthosoma spp.) based on morphological characteristics and isozymes analysis; (ii) to know the correlation between its genetic space based on morphological characteristics and its genetic resemblance based on isozymes-banding pattern. This research results were analyzed and described by descriptive qualitative methods. Morphological observation was carried out in sub-District of Galur, Lendah and Girimulyo, Kulonprogo District, Yogyakarta. Morphological data of the kimpul plant was explored descriptively and then made dendogram. Data of isozymic banding pattern were analyzed quantitatively based on the appearance of the band on the gel, and qualitatively based on the thickness of the band formed, and then made dendogram. The correlation, between its genetic distance based on morphological characteristics and its genetic resemblance based on isozymes-banding pattern, were then analyzed grounded on coefficient correlation between product- moment and goodness of it criteria based on correlation. The results pointed out that morphologically, on eight observed samples which were consist of four different types (species), each Xanthosoma from different locations did not indicate obvious differences. Esterase was formed four different banding-patterns, Glutamate Oxaloacetate Transaminase indicated eight different banding-patterns, and Peroxidase indicated seven different banding-patterns. Correlation between morphological data and data from EST and GOT isozymic banding pattern were very good (0.967918 and 0.937113), While, the correlations between morphological data and POD isozymes were good (0.892721). Key words: kimpul, Xanthosoma, morphological characteristic, isozyme

Protein expression on Cr resistant microorganism using electrophoresis method

Abstrak. Fatmawati U, Suranto, Sajidan. 2009. Ekspresi protein pada mikroorganisme resisten Cr dengan metode elektroforesis. Nusantara Bioscience 1: 31-37. Krom heksavalen (Cr(VI)) dikenal sebagai logam berat beracun, sehingga perlu direduksi menjadi Cr(III) yang lebih rendah toksisitasnya. Pseudomonas aeruginosa, Pseudomonas putida, Klebsiella pneumoniae, Pantoea sp. dan Saccharomyces cerevisiae adalah mikroorganisme resisten dan mampu mereduksi Cr(VI). Tujuan penelitian ini adalah mengetahui kemampuan mikroorganisme dalam mengurangi Cr(VI) dan mengetahui pola pita protein antara mikroorganisme resisten Cr(VI) dan mikroorganisme tidak resisten yang diinokulasi pada medium kaldu LB. SDS-PAGE digunakan untuk mengetahui ekspresi protein, sementara konsentrasi Cr(VI) diidentifikasi dengan metode 1,5 difenilkarbazid. Data kuantitatif dianalisis dengan ANAVA dua faktorial dilanjutkan dengan uji jarak berganda Duncan pada taraf 1%. Data kualitatif yaitu ekspresi protein dianalisis dengan mobilitas relatif (Rf). Hasil penelitian menunjukkan bahwa kemampuan mikroorganisme dalam mereduksi Cr(VI) pada konsentrasi awal 0.5 ppm, 1 ppm, 5 ppm dan 10 ppm berbeda-beda, persentase rata-rata kemampuan masing-masing mikroorganisme dalam mereduksi Cr(VI) adalah: P. putida (65%) > S. cerevisiae (64,45%) > P. aeruginosa (60,73%) > Pantoea sp. (50,22%) > K. pneumoniae (47,82%) > tanpa mikroorganisme (34,25%). Penambahan mikroorganisme secara nyata mempengaruhi reduksi Cr(VI). SDS-PAGE menunjukkan bahwa ekspresi protein antara mikroorganisme resisten dan tidak resisten tidak berbeda, tetapi mikroorganisme resisten memiliki lebih banyak protein (pita protein lebih tebal). Kata kunci: logam berat Cr, mikroorganisme, protein, elektroforesis

Kontribusi Naungan Pohon Terhadap Kepadatan Cacing Tanah

This research aims at exploring the contribution of the shade of treetowards the density of earthworms. The convertionof forest into a farmland causeda change in the shade of tree from a closed into an open ecosystem that was predicted to be followed by the decrease of earthworms density. The kinds of land thatis used for research are forest, complex agroforestry, simple agroforestry, teak monoculture, teak-accacia polyculture, and peanut crops. The data were analyzed quantitatively using statistical methods by SPSS 0.16. The results show that the wide oftree shade contributes to the density of earthworms on rainy season as much as 71.5% and also contributes to the density of earthworms on dry season as much as 52.2%. From the results, it can be concluded that the shade of tree has a strong role towards the density of earthworms

Bioinformatika: Trend dan Prospek dalam Pengembangan Keilmuan Biologi

PENDAHULUAN Tulisan ini terutama dimaksudkan untuk pengguna awal bioinformatika, termasuk mereka yang selama ini belum mengenal bioinformatika dan tertarik untuk memulainya[1]. Tapi bagi yang selama ini sudah menggeluti bioinformatika secara otodidak melalui internet terutama, cenderung mengalami kesalahan bila tidak memilki dasar-dasar yang kuat terhadap biologi molekuler atau menemui kebuntuan untuk melihat permasalahan biologi yang bisa dipecahkan dengan bioinformatika. Maka dari itu dengan penjelasan agak mendalam tentang prinsip biologi molekuler termasuk bagaimana data biologi molekuler itu didapatkan, diharapkan pembaca makalah ini bisa lebih optimal menggunakan bioinformatika khususnya menunjang pengembangan keilmuan biologi di Tanah Air[2]. [1] Witarto, A.B. Bioinformatika: Mengawinkan teknologi informasi dengan bioteknologi. Trendnya di dunia dan prospeknya di Indonesia. Disampaikan pada Seminar Seminar Teknologi Informasi diselenggarakan oleh MIFTA, Bogor, 9 Januari 2003. Bisa diunduh dari witarto.wordpress.com. [2] Witarto, A.B. Bioinformatics in Indonesia. Disampaikan pada First ASEAN-India Workshop on Bioinformatics di Center for DNA Fingerprinting and Diagnostics, Hyderabad, India, 7-11 November 2005. Bisa diunduh dari witarto.wordpress.co

Kimpul (Xanthosoma Spp.) Characterization Based on Morphological Characteristic and Isozymic Analysis

Nurmiyati, Sugiyarto, Sajidan. 2009. Kimpul (Xanthosoma spp.) characterization based on morphological characteristic andisozymic analysis. Nusantara Bioscience 1: 138-145. This research is aimed: (i) to know the variety of kimpul (Xanthosoma spp.) basedon morphological characteristics and isozymes analysis; (ii) to know the correlation between its genetic space based on morphologicalcharacteristics and its genetic resemblance based on isozymes-banding pattern. This research results were analyzed and described bydescriptive qualitative methods. Morphological observation was carried out in sub-District of Galur, Lendah and Girimulyo, KulonprogoDistrict, Yogyakarta. Morphological data of the kimpul plant was explored descriptively and then made dendogram. Data of isozymicbanding pattern were analyzed quantitatively based on the appearance of the band on the gel, and qualitatively based on the thickness ofthe band formed, and then made dendogram. The correlation, between its genetic distance based on morphological characteristics and itsgenetic resemblance based on isozymes-banding pattern, were then analyzed grounded on coefficient correlation between productmomentand goodness of it criteria based on correlation. The results pointed out that morphologically, on eight observed samples whichwere consist of four different types (species), each Xanthosoma from different locations did not indicate obvious differences. Esterasewas formed four different banding-patterns, Glutamate Oxaloacetate Transaminase indicated eight different banding-patterns, andPeroxidase indicated seven different banding-patterns. Correlation between morphological data and data from EST and GOT isozymicbanding pattern were very good (0.967918 and 0.937113), While, the correlations between morphological data and POD isozymes weregood (0.892721)

Variation of Morphological and Protein Pattern of Cassava (Manihot Esculenta) Varieties of Adira1 and Cabak Makao in Ngawi, East Java

Tribadi, Suranto, Sajidan. 2009. Variation of morphological and protein pattern of cassava (Manihot esculenta) varieties ofAdira1 and Cabak makao in Ngawi, East Java. Nusantara Bioscience 2: 14-22. This research is intended to find out the morphologicaland anatomical variation as well as the protein band pattern of cassava (Manihot esculenta Crantz) widely spread in three different areasof height. The sample collecting is done using simple random sampling in the three different areas of height that is 50, 300, 1000 metersasl in Ngawi District, East Java while the analysis of protein band pattern is done using SDS-PAGE. The result of the reseach ofmorphology and anatomy is analyzed descriptively and presented in the form of tabels, histograms and figures. The analysis of proteinband pattern is done using quantitative and qualitative analysis that is based on the appearance or not the gel band pattern by countingthe molecular weights based on code marker S 8445 and qualitative method based on the quality of the band formed. The band patternformed is istimated and presented in the form of zimogram. The result of the research shows that the height of the cultivating site verymuch influences toward variations of root, stem and leaf morphology. The longest root is at 50 meter heights asl (Cabak makao localvariety, the widest stem diameter is at 50 meters asl (Cabak makao local variety) the longest leaf and branch is at 300 meters asl (Cabakmakao local variety) and 1000 meters asl (Cabak makao local variety). There is no difference of anatomy in the root, stem and leaf andno difference of protein band pattern either in Adira1 or Cabak makao local variet

Pengembangan Modul Biologi Berbasis Discovery Learning (Part of Inquiry Spectrum Learning-wenning) Pada Materi Bioteknologi Kelas XII IPA Di SMA Negeri 1 Magelang Tahun Ajaran 2014/2015

Penelitian dan pengembangan modul dilakukan bertujuan untuk mengetahui karakteristik, kelayakan prototype dan keefektifan modul biologi berbasis Discovery Learning (part of Inquiry spectrum learning-Wenning) pada materi bioteknologi terhadap hasil belajar siswa kelas XII IPA di SMA Negeri 1 Magelang. Metode penelitian yang digunakan adalah metode penelitian dan pengembangan (R&D) Borg dan Gall (1983) yang dimodifikasi. Model pengembangan modul mengadaptasi model ADDIE (Analyze, Design, Develop, Implement dan Evaluate). Instrumen yang digunakan berupa: angket, observasi, penilaian diri sendiri, penilaian antar teman, dan tes. Uji lapangan operasional menggunakan Post-test only design Analisis data menggunakan analisis deskriptif dan menggunakan uji Independent Sample T-test. Berdasarkan hasil penelitian didapatkan bahwa; karakteristik modul hasil pengembangan adalah modul dilengkapi dengan basis model Discovery Learning, menekankan pada kerja sama kelompok dalam penemuan konsep bukan individu dan modul sesuai dengan tuntutan kurikulum 2013; kelayakan modul biologi berbasis Discovery Learning diperoleh skor rata-rata 86.42 dengan berkategori “sangat baik”; dan modul biologi berbasis Discovery Learning efektif untuk memberdayakan hasil belajar dari aspek sosial, aspek keterampilan dan aspek pengetahuan. Simpulan dari penelitian ini adalah modul biologi hasil pengembangan memiliki karakteristik dilengkapi basis model Discovery Learning yang menekankan pada kerja sama kelompok layak digunakan dan dapat memberdayakan aspek sosial, aspek keterampilan dan aspek pengetahuan

Characterisation of taro (Colocasia esculenta) based on morphological and isozymic patterns markers

Trimanto, Sajidan, Sugiyarto. 2011. Characterization of taro (Colocasia esculenta) based on morphological and isozymic patterns markers. Nusantara Bioscience: 7-14. The aims of this research were to find out: (i) the variety of Colocasia esculenta based on the morphological characteristics; (ii) the variety of C. esculenta based on the isozymic banding pattern; and (iii) the correlation of genetic distance based on the morphological characteristics and isozymic banding pattern. Survey research conducted in the Karanganyar district, which include high, medium and low altitude. The sample was taken using random purposive sampling technique, including 9 sampling points. The morphological data was elaborated descriptively and then made dendogram. The data on isozymic banding pattern was analyzed quantitatively based on the presence or absence of bands appeared on the gel, and then made dendogram. The correlation based on the morphological characteristics and isozymic banding pattern were analyzed based on the product-moment correlation coefficient with goodness of fit criterion. The result showed : (i) in Karanganyar was founded 10 variety of C. esculenta; (ii) morphological characteristics are not affected by altitude; (iii) isozymic banding pattern of peroxides forms 14 banding patterns, esterase forms 11 banding patterns and shikimic dehydrogenase forms 15 banding patterns; (iv) the correlation of morphological data and the isozymic banding pattern of peroxidase has good correlation (0.893542288) while esterase and shikimic dehydrogenase isozymes have very good correlation (0.917557716 and 0.9121985446); (v) isozymic banding pattern of data supports the morphological character data