444 research outputs found

    Cu2+ uptake by Chlorococcum hemicolum - A Xeric Chlorophycean Alga

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    Bioremediation of copper by xeric chlorophycean bioremediator, Chlorococcum hemicolum was investigated. The growth rates at various concentrations of Cu2+ were assessed in terms of protein level and 8 mg L-1 (37.67 % level in growth kinetics) is the tolerance limit. Absorption/adsorption kinetics was estimated after 240 hrs of Cu2+ treatments. Absorptions were higher than adsorption with maximum accumulation factor (AF) of 1.40. The Cu2+ concentration and absorption were linearly related (r = 0.99; p>0.01). Other biochemical parameters like total sugar, chlorophyll and carotenoids were also quantified to correlate the state of metabolism and these exhibited reduction due to heavy metal stress

    Efficient Privacy on Personalized Web Search Using Web Transformation Technique in User Profile

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    The time required for query processing over the internet is high due to the massively increasing amount of data on the server. Sometimes we may get irrelevant information as a result for a query. So we go for Personalized Web Search (PWS) to make the query processing good. In PWS, the query processing is done with the help of user profile. The user profile is created in two manners namely implicit and explicit. The implicit method creates the user profile from user’s browser histories, email, document and etc., without any effort from the user. Through this method the profile created with some user’s personal and secret information. Exposure of secret information on web leads to the privacy problem. In another way that the profile was created by explicit method. In this method the users requested to create their profile manually on the web. After profile creation the query processing is takes place. At each time a query is generated by a user that is combined with the personalized profile to generate a personalized query. Now the generalized query is send to the server. The server process the query then ranks the collected information. Finally the results are given to the client side and viewed to the user. The profile is updated in both ways at each time of query processing (automatically) and also by the manual update. To increase the privacy protection the profile details is reviewed at users own time. They can hide their secret information from the profile. Each profile updating process checks the newly added field information with the already hided field information. If any newly added field information matches with the personalized information then a notification is generated automatically to alert the user to personalize their profile. DOI: 10.17762/ijritcc2321-8169.15060

    Mediators of pre-mRNA splicing regulate sister chromatid cohesion in mammalian cells

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    The ‘endless forms most beautiful’ that populate our planet rely on the process of cell division to ensure equal segregation of the cellular content including the DNA to the two daughter cells. The accurate segregation of chromosomes in eukaryotes relies on connection between replicated sister chromatids, a phenomenon known as sister chromatid cohesion. Sister chromatid cohesion is mediated by a conserved ring-like protein complex known as cohesin. Defects in this process can promote aneuploidy and contribute to meiotic segregation errors with adverse consequences for developing embryos. Despite numerous advances into understanding cell division at the molecular level, we still lack a comprehensive list of the participating proteins and complexes. The aim of this thesis was to use available functional genomic and proteomic data to identify novel regulators of mitosis in human cells. Using an RNAi approach, we identified a set of factors involved in pre-mRNA splicing whose depletion prevents successful cell division. Loss of these splicing factors leads to a failure in chromosome alignment and to a protracted mitotic arrest that is dependent on the spindle assembly checkpoint. This mitotic phenotype was accompanied by a dramatic loss of sister chromatid cohesion that we could show happens as soon as DNA replication. While depletion of pre-mRNA splicing mediators had no effect on cohesin loading onto chromatin, it prevented the stable association of cohesin with chromatin. Immunoblotting revealed that the depletion of splicing factors caused a 5-fold reduction in the protein levels of Sororin, a protein required for stable association of cohesin with chromatin in post-replicative cells. Further analysis suggests erroneous splicing of Sororin pre-mRNA upon depletion of splicing factors. Importantly, the sister chromatid cohesion loss caused by depletion of splicing factors could be suppressed by a Sororin transgene that lacks introns. Our results suggest that that pre-mRNA splicing of Sororin is a rate-limiting step in the maintenance of sister chromatid cohesion in human cells. Our work reveals that a primary cellular pathology of compromised pre-mRNA splicing is a mitotic arrest accompanied by split sister chromatids. Our work linking splicing and sister chromatid cohesion has implications for the pathology of Chronic Lymphocytic Leukemia (CLL). One of the splicing factors that we implicate in sister chromatid cohesion is SF3B1, whose gene is one of the most frequently mutated genetic drivers found in CLL patients

    1-Methyl-3-(2-methyl­phen­yl)-3a-nitro-1,2,3,3a,4,9b-hexa­hydro­chromeno[4,3-b]pyrrole

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    The asymmetric unit of the title compound, C19H20N2O3, contains two independent mol­ecules in both of which the pyrrolidine ring adopts an envelope conformation, but with a C atom as the flap in one mol­ecule and the N atom in the other. The pyran ring adopts a half-chair conformation in both mol­ecules. In the crystal, mol­ecules are linked via C—H⋯O hydrogen bonds and C—H⋯π inter­actions

    1-(2-Azido­acet­yl)-3-methyl-2,6-diphenyl­piperidin-4-one

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    In the title compound, C20H20N4O2, the piperidine ring adopts a distorted boat conformation. The two phenyl rings form dihedral angles of 82.87 (1) and 84.40 (1)° with respect to the piperidine ring. The crystal packing is stabilized by inter­molecular C—H⋯O and C—H⋯N inter­actions

    1-(2-Bromo­acet­yl)-3-methyl-2,6-diphenyl­piperidin-4-one

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    In the title compound, C20H20BrNO2, the piperidone ring adopts a boat conformation. The phenyl rings are oriented at dihedral angles of 97.8 (2) and 96.0 (1)° with respect to the best plane through the piperidine ring. The dihedral angle between the two phenyl rings is 49.7 (1)°. In the crystal, bifurcated C—H⋯O hydrogen bonds form a R 2 1(7) ring motif, linking the mol­ecules into centrosymmetric dimers

    Ethyl 4-hy­droxy-2,6-diphenyl-1-(2-thio­morpholino­acet­yl)-1,2,5,6-tetra­hydro­pyridine-3-carboxyl­ate

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    In the title compound, C26H30N2O4S, the thio­morpholine ring adopts a chair conformation whereas the tetra­hydro­pyridine ring is in a half-chair conformation. The dihedral angle between the two phenyl rings is 33.3 (2)°. A strong intra­molecular O—H⋯O hydrogen bond generates an S(6) motif. In the crystal, mol­ecules are linked by inter­molecular C—H⋯O hydrogen bonds, generating a ribbon-like structure propagating along the a axis

    ETHNOPHARMACOLOGICAL STUDIES OF TRIBULUS TERRESTRIS (LINN). IN RELATION TO ITS APHRODISIAC PROPERTIES

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    Synergism and antagonism impact of different plant metabolites present in crude fruit extract of Tribulus terrestris ‘the herbal Viagra’ have been studied. Variability in plant composition, biomass and metabolites concentration in different modules was significantly contributed by spatial factor. However the edhaphic parameters also changes with both spatial and temporal factors significantly. Fruit is the officinal part and the fruit production significantly related with soil nitrogen (

    3-(2-Meth­oxy­naphthalen-1-yl)-2-benzofuran-1(3H)-one

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    The asymmetric unit of the title compound, C19H14O3, contains two crystallographically independent mol­ecules in which the dihedral angles between the naphthalene and benzofuran ring systems are 76.49 (7) and 86.17 (7)°. In the crystal, mol­ecules are linked by inter­molecular C—H⋯O hydrogen-bonding inter­actions into chains running parallel to the a axis. In addition, the crystal packing is stabilized by C—H⋯π inter­actions

    Methyl (2E)-2-{[(2-methyl­quinolin-8-yl)­oxy]meth­yl}-3-(thio­phen-2-yl)acrylate

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    In the mol­ecule of the title compound, C19H17NO3S, the dihedral angle formed by the quinoline ring system and the thio­phene ring is 83.15 (8)°. In the crystal, C—H⋯O hydrogen bonds link the mol­ecules into a C(8) chain running along the b axis. The packing of the mol­ecules is further influenced by C—H⋯π inter­actions
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