Additional file 1 of The influence of adipose-derived stromal vascular fraction cells on the treatment of knee osteoarthritis

Additional file 1. Each subscale score of Western Ontario and McMaster Universities Osteoarthritis Index, visual analog scale, Japanese Knee Osteoarthritis Measure, and Knee injury and Osteoarthritis Score

Supplemental Material, sj-docx-1-cll-10.1177_09636897211067454 - Comparison of Clinical and Imaging Outcomes of Different Doses of Adipose-Derived Stromal Vascular Fraction Cell Treatment for Knee Osteoarthritis

Supplemental Material, sj-docx-1-cll-10.1177_09636897211067454 for Comparison of Clinical and Imaging Outcomes of Different Doses of Adipose-Derived Stromal Vascular Fraction Cell Treatment for Knee Osteoarthritis by Masanori Tsubosaka, Tomoyuki Matsumoto, Satoshi Sobajima, Takehiko Matsushita, Hideki Iwaguro and Ryosuke Kuroda in Cell Transplantation</p

Additional file 2: of Gemcitabine and docetaxel combination chemotherapy for advanced bone and soft tissue sarcomas: protocol for an open-label, non-randomised, Phase 2 study

Procedure for implementation of study monitoring. (DOCX 19 kb

Additional file 1: of Gemcitabine and docetaxel combination chemotherapy for advanced bone and soft tissue sarcomas: protocol for an open-label, non-randomised, Phase 2 study

Data manegement and informed consent procedure. (DOCX 23 kb

DS_10.1177_0363546519879692 – Supplemental material for The Anterolateral Structure of the Knee Does Not Affect Anterior and Dynamic Rotatory Stability in Anterior Cruciate Ligament Injury: Quantitative Evaluation With the Electromagnetic Measurement System

Supplemental material, DS_10.1177_0363546519879692 for The Anterolateral Structure of the Knee Does Not Affect Anterior and Dynamic Rotatory Stability in Anterior Cruciate Ligament Injury: Quantitative Evaluation With the Electromagnetic Measurement System by Daisuke Araki, Takehiko Matsushita, Yuichi Hoshino, Kanto Nagai, Kyohei Nishida, Hideyuki Koga, Tomomasa Nakamura, Mai Katakura, Takeshi Muneta and Ryosuke Kuroda in The American Journal of Sports Medicine</p

P21 Deficiency Delays Regeneration of Skeletal Muscular Tissue

<div><p>The potential relationship between cell cycle checkpoint control and tissue regeneration has been indicated. Despite considerable research being focused on the relationship between p21 and myogenesis, p21 function in skeletal muscle regeneration remains unclear. To clarify this, muscle injury model was recreated by intramuscular injection of bupivacaine hydrochloride in the soleus of p21 knockout (KO) mice and wild type (WT) mice. The mice were sacrificed at 3, 14, and 28 days post-operation. The results of hematoxylin-eosin staining and immunofluorescence of muscle membrane indicated that muscle regeneration was delayed in p21 KO mice. <i>Cyclin D1</i> mRNA expression and both Ki-67 and PCNA immunohistochemistry suggested that p21 deficiency increased cell cycle and muscle cell proliferation. F4/80 immunohistochemistry also suggested the increase of immune response in p21 KO mice. On the other hand, both the mRNA expression and western blot analysis of <i>MyoD</i>, <i>myogenin</i>, and <i>Pax7</i> indicated that muscular differentiation was delayed in p21KO mice. Considering these results, we confirmed that muscle injury causes an increase in cell proliferation. However, muscle differentiation in p21 KO mice was inhibited due to the low expression of muscular synthesis genes, leading to a delay in the muscular regeneration. Thus, we conclude that p21 plays an important role in the <i>in vivo</i> healing process in muscular injury.</p></div

Additional file 1 of Altered microRNA profile during fracture healing in rats with diabetes

Additional file 1:. Figure S

Comparison of muscle weight/body weight.

<p>± Standard error.</p><p>Comparison of muscle weight/body weight.</p

Immunofluorescence analysis of muscle basement membrane and plasma membrane.

<p>The expression of DAPI, laminin, and dystrophin was examined in wild-type (WT; upper panel) and p21 knockout (KO; lower panel) mice. (A) Expression in control, (B) Expression in 3 days, (C) Expression in 14 days, and (D) Expression in 28 days post-operation. (Scale bar = 50μm). (E) Quantification of laminin expression, (F) Quantification of dystrophin expression. (A-F) The injured membrane structure was almost repaired 14 days after injury in WT group, but not in the p21 KO group (p < 0.05).</p

Western blot analysis of myogenic differentiation-related proteins.

<p>WT: wild type, KO: knockout. The expression levels of <i>MyoD</i>, <i>myogenin</i>, and <i>Pax7</i> was increased 3 days after injury in WT mice, but not in p21KO mice.</p