3,417 research outputs found

    Determination of Soluble Sugars in Arabidopsis thaliana Leaves by Anion Exchange Chromatography

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    Determination of soluble sugars is basic for the study of carbon metabolism in plants. Soluble sugar quantitation can be achieved by enzymatic methods implying different coupled reactions. Here we describe a simple method that allows rapid determination of the most abundant soluble sugars (glucose, fructose and sucrose) in Arabidopsis leaves by anion exchange chromatography. We have applied this method to study the levels of soluble sugars during the photoperiodic transition to flowering (Ortiz-Marchena et al., 2014).Espa√Īa, MINECO projects CSD2007-00057, BIO2008-02292, and BIO2011-28847-C02-00Espa√Īa, Junta de Andaluc√≠a P06-CVI-01450 and P08-AGR-0358

    Internet de las cosas para mejorar la capacidad de análisis, síntesis y evaluación

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    La asignatura Redes de Computadores I, que se imparte en el segundo cuatrimestre del primer curso del Grado en Inform√°tica de la UCLM, tiene como una de las competencias asignadas la de capacidad de an√°lisis, s√≠ntesis y evaluaci√≥n. Para hacer m√°s atractiva la asignatura, despu√©s del primer tema de introducci√≥n, se decidi√≥ incluir un tema de Internet, que repasara brevemente su historia, evoluci√≥n, aplicaciones, etc. Es en este tema donde se exponen los conceptos de Internet del Futuro y de Internet de las cosas (Internet of Things, IoT). IoT plantea que todos los objetos de la vida cotidiana puedan disponer de una direcci√≥n IP y puedan estar accesibles desde cualquier parte. Estos conceptos realmente entusiasman a los alumnos. Despu√©s de dos a√Īos observando esta situaci√≥n se decidi√≥ proponer una actividad relacionada con este tema y que sirviese como entrenamiento de la competencia de capacidad de an√°lisis, s√≠ntesis y evaluaci√≥n. Se propuso un trabajo en grupo para la elaboraci√≥n de p√≥steres relacionados con IoT. En concreto los temas a elegir ser√≠an: una visi√≥n general de IoT, IPv6, identificaci√≥n por radio frecuencia (RFID) y aplicaciones relacionadas. El objetivo final ser√≠a realizar una exposici√≥n permanente de los p√≥steres sobre IoT. Se decidi√≥ realizar esta actividad porque en los p√≥steres la capacidad de s√≠ntesis y an√°lisis de la informaci√≥n es algo fundamental. Cada uno de los grupos elegir√≠a un tema particular y dispondr√≠a de un tiempo para la realizaci√≥n del p√≥ster. Se realizar√≠an tres reuniones con el profesor para ir comprobando la evoluci√≥n del mismo y la resoluci√≥n de los posibles problemas o dudas. Transcurrido el tiempo, los grupos presentar√≠an en clase sus p√≥steres durante 5 minutos. Despu√©s de esta presentaci√≥n, los alumnos elegir√≠an los mejores p√≥steres para la exposici√≥n, mediante una votaci√≥n que puntuar√≠a distintos aspectos de inter√©s (gr√°ficos, organizaci√≥n de la informaci√≥n y explicaci√≥n del p√≥ster). La evaluaci√≥n se realizar√≠a por los propios alumnos. Tras el periodo de votaci√≥n y elecci√≥n de los mejores trabajos se pudo montar la exposici√≥n en el pasillo del laboratorio de redes. La experiencia ha sido todo un √©xito, los alumnos se volcaron en la realizaci√≥n de los p√≥steres y la calidad de los mismos fue muy alta. La exposici√≥n est√° en uno de los accesos principales de la escuela, por el que todos los d√≠as circulan gran cantidad de alumnos y profesores. Ha llamado la atenci√≥n de todos y se han recibido bastantes felicitaciones, tanto por el personal universitario local como por el visitante.SUMMARY -- Computer Networks I is a subject taught in the second semester of the first year of the Degree in Computer Science. One of the generic skills assigned to this subject is the ability of analysis, synthesis and evaluation. To make the course more attractive, a unit concerning Internet was included, which briefly reviews the history, evolution, and applications and current and future trends. It is in this unit where the concept of Internet of Things (IoT) is presented. Students are enthusiastic about this concept, so that, we decided to propose the development of posters related to IoT as teamwork. In particular, the topics were: an overview of IoT, IPv6, radio frequency identification (RFID) and IoT applications. This activity was proposed due to the ability to synthesize and analyze information is essential in the developing of posters. Each group chose a topic and was assigned a deadline for the submission of its poster. Three meetings with the teacher were scheduled to check the evolution of the poster and solve any problems or question. After the deadline, each group presents its poster for 5 minutes. Following these presentations, students scored each poster using the teacher‚Äôs rubric with the aim to choose the best posters for an exhibition. This rubric included different aspects of interest (graphics, organization of information and explanation of the poster). The students themselves conducted the evaluation. After the voting period, the top posters were chosen and an exhibition was held in the lobby of networks laboratory. The experience has been a success; students threw themselves into performing of the posters and the quality of them was very high. The exhibition is located in one of the main entrances to the school, where many students and teachers move every day. The exhibition has attracted the attention of everyone and teachers have received many compliments on it, both from the local university staff and visitors

    Purification of Starch Granules from Arabidopsis Leaves and Determination of Granule-Bound Starch Synthase Activity

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    Starch constitutes the most important carbon reserve in plants and is composed of branched amylopectin and linear amylose. The latter is synthesized exclusively by the Granule-Bound Starch Synthase (GBSS, EC 2.4.1.21). Here we report a readily reproducible, specific and highly sensitive protocol, which includes the isolation of intact starch granules from Arabidopsis thaliana leaves and the subsequent determination of GBSS activity. We have applied this method to study GBSS activity in diurnal cycles in vegetative growth and during the photoperiodic transition to flowering in Arabidopsis (Tenorio et al., 2003; Ortiz-Marchena et al., 2014).Espa√Īa,MINECO CSD2007-00057, BIO2008-02292, and BIO2011-28847-C02-00Espa√Īa, Junta de Andaluc√≠a P06-CVI-01450 and P08-AGR-0358

    CE-315 : A New Interacting Double-degenerate Binary Star

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    We present spectroscopic observations of object CE-315 revealing a blue continuum with strong emission lines. Most of the detected lines are identified with He I or He II in emission with a handful of faint lines of nitrogen. Notable is the complete absence of hydrogen lines. The He lines exhibit triple-peaked profiles with remakably broad widths, of about 2000 km/s (FWZP). The observations show that CE-315 is an interacting binary system with an orbital period of 65.1 +/- 0.7 minutes, and a mass ratio of 0.022. We conclude that the most likely scenario for this object is that of an accreting 0.77 solar mass white dwarf with a 0.017 solar mass helium white dwarf as mass donor.Comment: Astrophysical Journal, 20 page

    Medicina: Guía per a una docència universitària amb perspectiva de gènere

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    En ci√®ncies de la salut, a pesar que els conceptes de sexe i g√®nere al¬∑ludeixen a realitats diferents, amb freq√ľ√®ncia es confonen. A m√©s, algunes conseq√ľ√®ncies dels biaixos de g√®nere en el coneixement m√®dic, la gesti√≥ cl√≠nica i els serveis de salut responen a suposicions incorrectes. La Guia per a una doc√®ncia universit√†ria amb perspectiva de g√®nere de Medicina ofereix propostes, exemples de bones pr√†ctiques, recursos docents i eines de consulta que permeten incorporar un enfocament de g√®nere en els estudis universitaris en ci√®ncies de la salut i formar professionals en aquesta disciplina competents en mat√®ria de g√®nere.Ruiz Cantero, MT. (2018). Medicina: Gu√≠a per a una doc√®ncia universit√†ria amb perspectiva de g√®nere. Xarxa Vives d'Universitats. http://hdl.handle.net/10251/11908

    Strong Anisotropy in Liquid Water upon Librational Excitation using Terahertz Laser Fields

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    Tracking the excitation of water molecules in the homogeneous liquid is challenging due to the ultrafast dissipation of rotational excitation energy through the hydrogen-bonded network. Here we demonstrate strong transient anisotropy of liquid water through librational excitation using single-color pump-probe experiments at 12.3 THz. We deduce a third order response of chi^3 exceeding previously reported values in the optical range by three orders of magnitude. Using a theory that replaces the nonlinear response with a material response property amenable to molecular dynamics simulation, we show that the rotationally damped motion of water molecules in the librational band is resonantly driven at this frequency, which could explain the enhancement of the anisotropy in the liquid by the external Terahertz field. By addition of salt (MgSO4), the hydration water is instead dominated by the local electric field of the ions, resulting in reduction of water molecules that can be dynamically perturbed by THz pulses

    A meta-analysis of amino acid őī\u3csup\u3e15\u3c/sup\u3eN trophic enrichment factors in fishes relative to nutritional and ecological drivers

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    The use of amino acid (AA) nitrogen stable isotopes (őī15N) from consumer tissues aims to provide precise estimates of trophic position (TP), but the drivers of AA isotope fractionation remain unclear. In particular, the main factors driving the variability in TEFAA among taxonomic groups and trophic levels remain largely unexplained, which challenges the application of universal values for TEFs. While the relationship between protein content and quality and TEFs has been examined, studies have yielded inconsistent results, and the role of protein and lipid nutritional requirements as well as feeding regime have not been considered. Likewise, drivers that influence physiological and nutritional processes have not been examined relative to TEFAA variation. We conducted a meta-analysis of controlled feeding experiments within a single group, teleosts fishes, to evaluate the relationship between five nutritional factors (protein and lipid content, protein and lipid content relative to nutritional requirements, and feeding regime) and three ecological drivers (diet type, life stage, and habitat type) on TEFAA. We considered a broad range of protein levels (8‚Äď71%) in diets and found no relationship between source TEFAAs and percent protein relative to nutritional requirements, whereas lipid content relative to nutrient requirements, feeding regime and habitat type partially explain the variability in TEFs of Lys, but not for Phe and Met TEFs. The variability for the latter was representative of robust source AAs. Among trophic AAs, Asp, Ile, Pro, and Leu TEFs were significantly higher in species from brackish than marine habitats possibly due to osmoregulation involvement. TEFGlu was sensitive to protein content and feeding regime within teleosts, but relatively constant when comparing TEFs among teleosts, non-teleosts, and all taxa. Our results indicate that TEFAA is less variable within a single taxon than among multiple taxa and that such variation is not negligible. Our results indicate that őī15NAA values could provide better TP estimates if using taxon-specific values, and highlights the need to explain the mechanisms of AA fractionation and quantify the variability in TEFs used during error propagation for TP estimates

    Table-top laser-based proton acceleration in nanostructured targets

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    The interaction of ultrashort, high intensity laser pulses with thin foil targets leads to ion acceleration on the target rear surface. To make this ion source useful for applications, it is important to optimize the transfer of energy from the laser into the accelerated ions. One of the most promising ways to achieve this consists in engineering the target front by introducing periodic nanostructures. In this paper, the effect of these structures on ion acceleration is studied analytically and with multidimensional particle-in-cell simulations.Weassessed the role of the structure shape, size, and the angle of laser incidence for obtaining the efficient energy transfer. Local control of electron trajectories is exploited to maximize the energy delivered into the target. Based on our numerical simulations, we propose a precise range of parameters for fabrication of nanostructured targets, which can increase the energy of the accelerated ions without requiring a higher laser intensity.This work has been partially supported by the Xunta de Galicia/FEDER under contract Agrup2015/11 (PC034) and by MINECO under contracts MAT2015-71119-R and FIS2015-71933-REDT. The authors would like to acknowledge the OSIRIS Consortium, consisting of UCLA and IST (Lisbon, Portugal) for the use of OSIRIS, for providing access to the OSIRIS framework. M Blanco also thanks the Ministry of Education of the Spanish government for the FPU fellowship. Camilo Ruiz also thanks MINECO project FIS2016-75652-P M Vranic acknowledges the support of ERC-2010-AdG Grant 267841 and LASERLAB-EUROPE IV‚ÄĒGA No. 654148. Simulations were performed at the Accelerates cluster (Lisbon, Portugal)S

    Gender inequality and violence against women in Spain, 2006-2014: towards a civilized society

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    ABSTRACT. Objective: Considering both the economic crisis of 2008 and the Gender Equality Law (2007), this study analyses the association between gender inequality in Spanish Autonomous Communities (AC) and intimate partner violence (IPV) from 2006 to 2014 in terms of socio-demographic characteristics. Methods: Ecological study in the 17 Spanish AC on the correlation between the reported cases by IPV and deaths and the Gender Inequality Index and its dimensions: empowerment, participation in the labour market and adolescent birth rates; and their correlation with Young People Not in Education, Employment or Training (NEET). Results: In 2006, IPV mortality rates were higher in autonomous communities with greater gender inequality than AC with more equality (4.1 vs. 2.5 √ó 106 women >14 years), as were reporting rates of IPV (OR = 1.49; 95% CI: 1.47-1.50). In 2014, the IPV mortality rates in AC with greater gender inequality fell to just below the mortality rates in AC with more gender equality (2.5 vs. 2.7 √ó 106 women >14 years). Rates of IPV reports also decreased (OR = 1.22; 95% CI: 1.20-1.23). Adolescent birth rates were most associated with IPV reports, which were also associated with the burden of NEET by AC ( 2006 = 0.494, 2014 = 0.615). Conclusion: Gender-sensitive policies may serve as a platform for reduced mortality and reports of IPV in Spain, particularly in AC with more gender inequality. A reduction of NEET may reduce adolescent birth rates and in turn IPV rates.RESUMEN. Objetivo: Considerando la crisis econ√≥mica de 2008 y la Ley de igualdad entre hombres y mujeres (2007), se analiza la asociaci√≥n entre la desigualdad de g√©nero de las comunidades aut√≥nomas (CCAA) espa√Īolas con la violencia del compa√Īero √≠ntimo (VCI) en 2006 y 2014, respecto a caracter√≠sticas sociodemogr√°ficas. M√©todos: Estudio ecol√≥gico en las 17 CCAA sobre la asociaci√≥n entre las muertes y denuncias por VCI y el √ćndice de Desigualdad de G√©nero y sus dimensiones: empoderamiento, participaci√≥n en el mercado laboral y tasas de fecundidad de adolescentes; y su asociaci√≥n con j√≥venes que ni estudian ni trabajan. Resultados: En 2006, las CCAA con mayor desigualdad de g√©nero sufrieron tasas de mortalidad por VCI superiores que las de mayor igualdad (4,1 frente a 2,5 √ó 106 mujeres >14 a√Īos), y tambi√©n las denuncias fueron m√°s (odds ratio [OR]: 1,49; intervalo de confianza del 95% [IC95%]: 1,47-1,50). En 2014, disminuyeron las tasas de mortalidad por VCI en las CCAA con mayor desigualdad de g√©nero, situ√°ndose ligeramente por debajo de las de m√°s igualdad (2,5 frente a 2,7 √ó 106 mujeres >14 a√Īos). Las denuncias tambi√©n disminuyeron (OR: 1,22; IC95%: 1,20-1,23). La fecundidad de las adolescentes es la dimensi√≥n m√°s asociada con las denuncias por VCI, asociadas al porcentaje de j√≥venes que ni estudian ni trabajan por CCAA ( 2006 = 0,494, 2014 = 0,615). Conclusi√≥n: Las pol√≠ticas con perspectiva de g√©nero pueden ser la base de la reducci√≥n de la mortalidad y de las denuncias por VCI en Espa√Īa, y a√ļn m√°s en las CCAA con m√°s desigualdad de g√©nero. Reducir la cantidad de j√≥venes que ni estudian ni trabajan puede disminuir la fecundidad adolescente y los √≠ndices de VCI
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