1 research outputs found
Discrimination of Cell Death Types with an Activatable Fluorescent Probe through Visualizing the Lysosome Morphology
Cell
death plays a vital role in body development, maintenance
of tissue function, and homeostasis. Accurate evaluation of cell death
types is of great importance for pharmacological and pathological
research. However, there is a lack of efficient fluorescent probes
to discriminate various cell states. Here, we design and synthesize
a novel activatable fluorescent probe PNE-Lyso to detect
intracellular pH and hexosaminidases with two kinds of fluorescence
signals. PNE-Lyso could distinguish dead cells from healthy
cells based on a dual-color mode by targeting the lysosome and evaluating
lysosomal hexosaminidase activity. Significantly, PNE-Lyso could also discriminate apoptotic and necrotic cells through visualizing
lysosome morphology that is adjusted by the integrity of the lysosome
membrane. Moreover, probe PNE-Lyso was successfully applied
to investigate the drug-induced cell death process. To the best of
our knowledge, this work is the first time cell death types have been
distinguished based on a single fluorescent probe