DataSheet_1_Vaginal Lactobacillus iners abundance is associated with outcome in antibiotic treatment of bacterial vaginosis and capable of inhibiting Gardnerella.pdf

Bacterial vaginosis is characterized as a polymicrobial dysbiosis with the loss of Lactobacillus spp. and growth of multiple anerobic bacteria, including Gardnerella, Prevotella and Atopobium ranked as the top three most abundant. A total of nine Gardnerella genomospecies have been identified, yet the association between their distribution or any exact Lactobacillus species with BV occurrence or prognosis remains controversial. A total of 308 patients and 62 healthy women who sought annual examinations were recruited, with 130 BV patients and 41 healthy women who met our inclusion criteria finally included. Vaginal samples were used for microscopic examination, 16S rRNA sequencing, bacterial culture and isolation. Isolates of Gardnerella vaginalis, Fannyhessae vaginae (used to be called Atopobium vaginae) and Lactobacillus iners were used for competition tests. We found that the relative abundances of Gardnerella, Prevotella and Atopobium were elevated in BV patients compared to healthy people (p0.05). Seven out of nine Gardnerella genomospecies were present in both BV patients and healthy women, and the relative abundances of all detected genomospecies were higher in BV patients (p<0.05). Cured patients possessed higher GS03 than intermediate and failed patients (p=0.005, 0.0337). L. iners was significantly higher in cured patients than in the other two groups (p=0.0021, p<0.0001), and its ability to inhibit the growth of G. vaginalis and F. vaginae was validated. In summary, seven Gardnerella genomospecies were detected in Chinese BV patients, but no association of its distribution and BV occurrence or prognosis was found. The relative abundance of L. iners was higher in cured patients, and its antimicrobial activity against G. vaginalis and F. vaginae was validated through in vitro inhibition experiment. L. iners could become a predictive indicator of clinical outcomes of BV patients, and its antimicrobial function might be beneficial to BV patients.</p

DataSheet_1_CAPRIN2 upregulation by LINC00941 promotes nasopharyngeal carcinoma ferroptosis resistance and metastatic colonization through HMGCR.pdf

Distant metastasis is the main cause of death in nasopharyngeal carcinoma (NPC) patients. There is an urgent need to reveal the underlying mechanism of NPC metastasis and identify novel therapeutic targets. The ferroptosis resistance and survival ability of extracellular matrix (ECM)-detached tumor cells are important factors in determining the success of distant metastasis. In this study, we found that CAPRIN2 contributes to the ferroptosis resistance and survival of ECM-detached NPC cells. Moreover, CAPRIN2 serves as a positive regulator of NPC cell migration and invasion. HMGCR, the key metabolic enzyme of the mevalonate pathway, was identified as the key downstream molecule of CAPRIN2, which mediates its regulation of ferroptosis, survival, migration and invasion of NPC cells. Lung colonization experiments showed that downregulation of the CAPRIN2/HMGCR axis resulted in reduced lung metastasis of NPC cells. Erastin treatment inhibited the ability of NPC cells to colonize the lungs, which was further enhanced by CAPRIN2/HMGCR axis downregulation. Regulated by upstream LINC00941, CAPRIN2 is abnormally activated in NPC, and its high expression is associated with a poor prognosis. In conclusion, CAPRIN2 is a molecular marker of a poor prognosis in NPC, and the LINC00941/CAPRIN2/HMGCR axis provides a new target for the treatment of NPC metastasis and ferroptosis resistance.</p

Relationship between ACS groups and the fractures.

<p>ACS  =  aortic calcifications score.</p><p>*p<0.01 vs G1.</p

Fracture-free survival according to baseline ACS.

<p>Survival of women in this study cohort during over 5 years of follow-up, according to the ACS at baseline: ACS in G1 (0), G2 (1–2), G3 (3–6), and G4(>6). Fracture incidence was significantly higher in G2, G3, and G4, compared to G1, at the end of the 5 years of follow-up (p<0.001, respectively).</p

Association between AC and non-vertebral fractures in Cox proportional hazard regression model.

<p>BMD = bone mineral density, vertebral and hip BMD; ACS = aortic calcifications score; SD = standard deviation.</p><p>Adjusted for age, BMI, BMD, history of two or more falls, current smoking, current drinking, previous fracture, hypertension, diabetes, total cholesterol, myocardial infarction, stroke, adiponectin, osteocalcin, leptin and 25(OH)D.</p

Baseline characteristics of the subjects who developed aortic calcification and who did not in the study of 1724 women.

<p>AC = aortic calcification; BMD = bone mineral density.</p

Incidence of vertebral and non-vertebral fractures per 100 person-years by age and site.

<p>Incidence of vertebral and non-vertebral fractures was significantly higher in the 60- to 69-year-old group, the 70- to 79-year-old group and the>80-year-old group compared to the 50- to 59-year-old group (p<0.001, respectively). In each group, the vertebral fracture prevalence was significantly higher than for non-vertebral fractures (p<0.001).</p

Pericellular Matrix Enhances Retention and Cellular Uptake of Nanoparticles

A hydrated gel-like pericellular matrix (PCM) covers the surface of all eukaryotic cells and plays a key role in many cellular events, but its effect on nanoparticle internalization has not been studied. Here, using cells with various PCM thicknesses and gold nanoparticles as probes, we demonstrate that, rather than being a barrier to all foreign objects, the PCM can entrap and accumulate NPs, restrict and slow down their diffusion, and enhance their cellular uptake efficiency. Moreover, this newly discovered PCM function consumes energy and seems to be an integral part of the receptor-mediated endocytosis process. These findings are important in understanding the delivery mechanisms of nanocarriers for biomedical applications

Pericellular Matrix Enhances Retention and Cellular Uptake of Nanoparticles

A hydrated gel-like pericellular matrix (PCM) covers the surface of all eukaryotic cells and plays a key role in many cellular events, but its effect on nanoparticle internalization has not been studied. Here, using cells with various PCM thicknesses and gold nanoparticles as probes, we demonstrate that, rather than being a barrier to all foreign objects, the PCM can entrap and accumulate NPs, restrict and slow down their diffusion, and enhance their cellular uptake efficiency. Moreover, this newly discovered PCM function consumes energy and seems to be an integral part of the receptor-mediated endocytosis process. These findings are important in understanding the delivery mechanisms of nanocarriers for biomedical applications

Realizing Wide-Temperature Reversible Ca Metal Anodes through a Ca²⁺-Conducting Artificial Layer

Room-temperature Ca deposition/stripping is impeded by the formation of ionic insulating interfaces. Electrolyte optimization could partially enhance Ca reversibility by tailoring the interfaces, but the precise regulation of the composition remains challenging. Herein, we construct an ex situ artificial layer on Ca metal via a facile displacement reaction between metal halides and Ca. These Ca-driven spontaneous layers with precisely controlled interfacial chemistry consist of a Ca metal alloy phase and a calcium halide matrix for conducting Ca2+ and insulating the electrons, as revealed by theoretical and experimental investigations. In particular, the Ca31Sn20/CaBr2 interface enables Ca metal anodes to achieve low polarization and humid air stability over a wide temperature range from −25 to +50 °C. This proof-of-concept work provides an alternative approach to boost Ca2+ diffusivity through customized interfacial chemistry regulation