206 research outputs found

    Micropropagation of Salvia wagneriana Polak and hairy root cultures with rosmarinic acid production

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    Salvia wagneriana Polak is a tropical species native to Central America, well adapted to grow in the Mediterranean basin for garden decoration. Micropropagation has been assessed from axillary shoots of adult plants using a Murashige and Skoog basal medium, with the addition of 1.33-μM 6-benzylaminopurine for shoot proliferation; the subsequent rooting phase occurred in plant growth regulator-free medium. The plants were successfully acclimatised with high survival frequency. Hairy roots were induced after co-cultivation of leaf lamina and petiole fragments with Agrobacterium rhizogenes and confirmed by PCR. The establishment and proliferation of the selected HRD3 line were obtained in hormone-free liquid medium and the production of rosmarinic acid (RA) was evaluated after elicitation. The analysis of RA was performed by LC-ESI-DAD-MS in the hydroalcoholic extracts. The addition of casein hydrolysate increased the RA production, whereas no enrichment was observed after the elicitation with jasmonic acid

    Experimental exploration of the origin of magnetostriction in single crystalline iron

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    The magnetostrictive atomic strain in a pure Fe single crystal was measured by differential x-ray absorption spectroscopy. The obtained tetragonal magnetostriction constant, (3/2)λ100, was determined to be 45 ppm, consistent with the previously reported theoretical value calculated from a spin-orbit coupling theory. These results provide a foundation for understanding the origin of magnetostriction in pure Fe as well as Fe-based binary alloys

    ANÁLISE DAS SITUAÇÕES ESTRESSORAS DE EQUIPES UNIVERSITÁRIAS DE VOLEIBOL

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    Introdução: O desempenho dos atletas de voleibol pode ser influenciado por fatores psicológicos, havendo a necessidade de investigações sobre determinadas situações potencialmente estressantes durante o jogo. Objetivo: Investigar os fatores produtores de estresse em atletas de equipes universitárias de voleibol durante a partida de voleibol. Métodos: Participaram da pesquisa 28 atletas universitários (21,63À1,75 anos de idade). O instrumento utilizado foi o Teste de Estresse Psíquico para o Voleibol (TEP-V) constituído de 18 situações típicas de estresse durante as partidas de voleibol, sendo analisados o nível de estresse, o comportamento provável e o nível de eficiência de tal comportamento. Valores percentuais foram utilizados para a análise estatística. Resultados: A situação “Minha equipe perde o ponto em um momento decisivo por um erro meu” foi considerada a mais estressante, com 39,3% considerando estressante e 53,6% como muito estressante. O comportamento provável mais indicado pelos atletas foi o de tentar se tranquilizar e se esforçar mais mediante as situações de estresse. Conclusão: Concluiu-se que os atletas se sentem potencialmente estressados em situações características de falha individual e momentos em que não possuem o controle sobre o jogo.

    Androgen receptor condensates as drug targets

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    Transcription factors are among the most attractive therapeutic targets, but are considered largely undruggable. Here we provide evidence that small molecule-mediated partitioning of the androgen receptor, an oncogenic transcription factor, into phase-separated condensates has therapeutic effect in prostate cancer models. We show that the phase separation capacity of the androgen receptor is driven by aromatic residues and short unstable helices in its intrinsically disordered activation domain. Based on this knowledge, we developed tool compounds that covalently attach aromatic moieties to cysteines in the receptors’ activation domain. The compounds enhanced partitioning of the receptor into condensates, facilitated degradation of the receptor, inhibited androgen receptor-dependent transcriptional programs, and had antitumorigenic effect in models of prostate cancer and castration-resistant prostate cancer in vitro and in vivo. These results establish a generalizable framework to target the phase- separation capacity of intrinsically disordered regions in oncogenic transcription factors and other disease-associated proteins with therapeutic intent

    Plant cell culture technology in the cosmetics and food industries : current state and future trends

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    The production of drugs, cosmetics, and food which are derived from plant cell and tissue cultures has a long tradition. The emerging trend of manufacturing cosmetics and food products in a natural and sustainable manner has brought a new wave in plant cell culture technology over the past 10 years. More than 50 products based on extracts from plant cell cultures have made their way into the cosmetics industry during this time, whereby the majority is produced with plant cell suspension cultures. In addition, the first plant cell culture-based food supplement ingredients, such as Echigena Plus and Teoside 10, are now produced at production scale. In this mini review, we discuss the reasons for and the characteristics as well as the challenges of plant cell culture-based productions for the cosmetics and food industries. It focuses on the current state of the art in this field. In addition, two examples of the latest developments in plant cell culture-based food production are presented, that is, superfood which boosts health and food that can be produced in the lab or at home

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