Efficacy of using a dual layer of membrane (d PTFE placed over collagen) for ridge preservation in fresh extraction sites: a micro‐computed tomographic study in dogs

Objective To assess if overbuilding the buccal plate or using a dual‐layer socket grafting technique prevents alveolar bone resorption and enhances final ridge width, height, and volume after tooth loss in an animal model. Material and methods In eight beagle dogs bilateral second (P2)‐, third (P3)‐, and fourth (P4) premolars were endodontically treated. All bilateral mandibular first premolars and distal roots of P2, P3, and P4 were hemisectioned and atraumatically extracted. Animals were randomly divided into four groups: (i) Control–Socket alone, (ii) Particulate allograft in the alveolum, socket covered with high‐density polytetrafluoroethylene ( dPTFE ) membrane and sutured over the alveolum, (iii) Particulate allograft in the alveolum and overbuilding the buccal plate, socket covered with dPTFE membrane and sutured over the alveolum, (iv) Particulate allograft in the alveolum and covered with dual layer ( dPTFE placed over collagen membrane), and sutured over the alveolum. After 16 weeks, the animals were sacrificed. Mandibular blocks of the jaws were assessed for bone volume ( BV ), vertical bone height ( VBH ), alveolar ridge thickness, and bone mineral density ( BMD ) using micro‐computed tomography. Results The BV in groups 1, 2, 3, and 4 was 169.5, 207.57, 242.4, and 306.1 mm 3 , respectively. The VBH in groups 1, 2, 3, and 4 was 4.2, 6.4, 6.2, and 7.3 mm, respectively. Ridge widths in groups 1, 2, 3, and 4 were 5.45 ± 0.75, 5.91 ± 0.86, 6.05 ± 0.63, and 6.28 ± 1.01 mm, respectively. There was no significant difference in BMD between the groups. Conclusions The RP using a dual layer of membrane following tooth extraction results in more BV , VBH , and alveolar ridge width as compared to when a single layer of membrane is used.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/100133/1/clr2526.pd

A randomized clinical trial evaluating the efficacy of the sandwich bone augmentation technique in increasing buccal bone thickness during implant placement surgery

Objectives Sandwich bone augmentation ( SBA ) has been proposed to augment the width of edentulous ridges for implant placement. This study aimed to investigate the effect of a membrane on SBA for the regeneration of buccal implant dehiscence defects. Material and methods Twenty‐six healthy patients, each with a single defect, were randomly assigned into two groups. Both groups received an inner and outer layer of mineralized human cancellous and cortical particulate allograft. In the test group, a bovine pericardium membrane covered the bone grafts, while no membrane was placed in the control group. Cone beam computed tomography ( CBCT ) scans were taken before and immediately after implant placement and at 6 months post‐surgery. Results All implants placed were successfully osseointegrated at 6 months. Clinical re‐entry measurements showed significant buccal bone gain in the test group compared with the control group ( P   0.05). Cone beam computed tomography analysis demonstrated significant buccal bone gain of 1.22 mm in the test group. Radiographic vertical bone loss at 1‐year post‐surgery showed no significant differences between the groups. Conclusion Sandwich bone augmentation is a predictable technique for regenerating buccal bone on implant dehiscence defects. Addition of a barrier membrane prevented significant horizontal buccal bone resorption as space was maintained more effectively when compared with sites treated without a membrane.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/106765/1/clr12171.pd

Effect of Flapless Surgery on Singleâ Tooth Implants in the Esthetic Zone: A Randomized Clinical Trial

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/141931/1/jper1747.pd

Risk Assessment of Lingual Plate Perforation in Posterior Mandibular Region: A Virtual Implant Placement Study Using Coneâ Beam Computed Tomography

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/141229/1/jper0129.pd

Optimized Cell Survival and Seeding Efficiency for Craniofacial Tissue Engineering Using Clinical Stem Cell Therapy

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/135593/1/sct320143121495.pd

Efficacy of Acellular Dermal Matrix and Coronally Advanced Flaps for the Treatment of Induced Gingival Recession Defects: A Histomorphometric Study in Dogs

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/141958/1/jper1172.pd

Hard and Soft Tissue Changes After Crestal and Subcrestal Immediate Implant Placement

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/141272/1/jper1112.pd

Bone Engineering of Maxillary Sinus Bone Deficiencies Using Enriched CD90+ Stem Cell Therapy: A Randomized Clinical Trial

Bone engineering of localized craniofacial osseous defects or deficiencies by stem cell therapy offers strong prospects to improve treatment predictability for patient care. The aim of this phase 1/2 randomized, controlled clinical trial was to evaluate reconstruction of bone deficiencies of the maxillary sinus with transplantation of autologous cells enriched with CD90+ stem cells and CD14+ monocytes. Thirty human participants requiring bone augmentation of the maxillary sinus were enrolled. Patients presenting with 50% to 80% bone deficiencies of the maxillary sinus were randomized to receive either stem cells delivered onto a β‐tricalcium phosphate scaffold or scaffold alone. Four months after treatment, clinical, radiographic, and histologic analyses were performed to evaluate de novo engineered bone. At the time of alveolar bone core harvest, oral implants were installed in the engineered bone and later functionally restored with dental tooth prostheses. Radiographic analyses showed no difference in the total bone volume gained between treatment groups; however, density of the engineered bone was higher in patients receiving stem cells. Bone core biopsies showed that stem cell therapy provided the greatest benefit in the most severe deficiencies, yielding better bone quality than control patients, as evidenced by higher bone volume fraction (BVF; 0.5 versus 0.4; p = 0.04). Assessment of the relation between degree of CD90+ stem cell enrichment and BVF showed that the higher the CD90 composition of transplanted cells, the greater the BVF of regenerated bone (r = 0.56; p = 0.05). Oral implants were placed and restored with functionally loaded dental restorations in all patients and no treatment‐related adverse events were reported at the 1‐year follow‐up. These results provide evidence that cell‐based therapy using enriched CD90+ stem cell populations is safe for maxillary sinus floor reconstruction and offers potential to accelerate and enhance tissue engineered bone quality in other craniofacial bone defects and deficiencies (Clinicaltrials.gov NCT00980278). © 2015 American Society for Bone and Mineral Research. © 2015 American Society for Bone and Mineral ResearchPeer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/112016/1/jbmr2464.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/112016/2/jbmr2464-sup-0009-SupLegend-S1.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/112016/3/jbmr2464-sup-0008-SupTab-S4.pd

The Influence of the Buccoâ Palatal Distance on Sinus Augmentation Outcomes

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/141630/1/jper1041.pd