MOESM1 of Insights into the genetic variation of maternal behavior and suckling performance of continental beef cows

Additional file 1. Description of the recorded traits. Description: Description of the way the three traits were recorded on a station

MOESM3 of Multi-breed and multi-trait co-association analysis of meat tenderness and other meat quality traits in three French beef cattle breeds

Additional file 3: Figure S2. (A) Number of overlapping SNPs. (B) Overlapping pathways. The figure represents the number of overlapping SNPs and pathways identified across the three beef cattle breeds

MOESM2 of Multi-breed and multi-trait co-association analysis of meat tenderness and other meat quality traits in three French beef cattle breeds

Additional file 2: Table S1. Comparison of genetic correlations between traits and SNP-based correlations (777K, AWM and 206 common genes). The data summarize the comparison between genetic correlations and SNP-based correlations across the three beef cattle breeds

Additional file 1: of Detection of quantitative trait loci for maternal traits using high-density genotypes of Blonde d’Aquitaine beef cattle

Number of genotyped animals in Blonde d’Aquitaine breed according to the chip density. (PNG 601 kb

MOESM5 of Multi-breed and multi-trait co-association analysis of meat tenderness and other meat quality traits in three French beef cattle breeds

Additional file 5: Table S3. Description of the genes that overlap with the QTL for Warner-bratzler shear force (WBSF) according to the Cattle QTLdb

Additional file 2: of Detection of quantitative trait loci for maternal traits using high-density genotypes of Blonde d’Aquitaine beef cattle

Strong evidence QTL for CS, PO, CSm, MY, S and WWm. (PDF 1422 kb

Supplementary material for <b>Genetic parameters, GWAS and selection perspective on gestation length in 16 French cattle breeds </b><i>by Jourdain et al., 2024</i>

The file contents all the supplementary materials mentionned in the article Genetic parameters, GWAS and selection perspective on gestation length in 16 French cattle breeds by Jourdain et al., 2024.Page 1 & 2 are presented Supplementary tables 1 & 2Page 3 to 34 are presented Supplementary figures 1 to 16, including the descriptive data of gestation length for each of the breeds used in the paper </p

Quantification of the length of paranodal section in cerebellar peduncles of non-affected and affected cattle.

<p>(A) Caspr positivity was concentrated in two paranodal compartments on either side of the node of Ranvier. The immunostaining was observed on myelinated fibres of various diameters. (Caspr green, Dapi blue). (B) The lengths of the paranodal region vary slightly; however, in affected white matter this length appeared more variable and greater within and around the demyelinating lesion. (C) Quantitative comparison and graphic representation of these lengths in WT controls, within and outside the lesions. Numbers of quantified paranodal sections in WT controls: 570; in affected cattle: inside lesion 115 paranodes and outside lesion 194 paranodes. Scale bar: (A) and (B) 40 μm.</p

The participating cells in lesions.

<p>(A) Prominent astrocytic reaction as evidenced by anti-GFAP antibody (GFAP, green; Dapi, blue). Astrocytic reaction, engulfing the lesion; a few astrocytic feet penetrated the lesion (<i>white asterisk</i>). (B) Microglial activation, immunolabelled by anti Iba1, reproduced similar topography as the astrocytic reaction by surrounding the lesion, and few microglial cell processes infiltrated the lesion (<i>white asterisk</i>) (Iba1, green; Dapi, blue). Concurrently, astrocytic and microglial cell activations are present not only around the lesions but also elsewhere. (C) Immunostaining of anti-oligodendrocyte specific protein (anti-OSP), a cell membrane oligodendrocyte marker (OSP, green; Dapi, blue. Big or confluent lesions (<i>white arrows</i>), where the centre is occupied by many OSP-positive and intricate processes of different shapes and sizes, which presumably depend on the plane section. (D) Double immunostaining showed that the two markers–cytoplasmic (MBP) (red) and membrane (OSP) (green)–of oligodendrocytes were present together in the demyelinating lesions and the centre of the plaque was double-immunostained (<i>white arrows</i>). (E) Anti-actin immunostaining (red), Dapi (blue), actin protein aggregates were also accumulated in the centre (<i>arrow</i>) of the plaque and in the cytoplasm of some surrounding cells (<i>white arrows</i>). (F) Double immunostaining of actin (red) and MBP (green) Dapi (blue). Within the core of the plaque with actin immunostaining, note the presence of oligodendrocyte processes (<i>white arrows</i>). Scale bar: (A) and (B) 30 μm, (C) 20 μm, (D) 50 μm, (E) and (F) 20 μm.</p

Cytological features of the lesions.

<p>Semi-thin blue toluidine stained section of white matter from spinal cord and cerebellum. (A) Cerebellar white matter less affected region showing a small lesion or “pre-plaque” (<i>arrow</i>) as a hypertrophied cell with histological characteristics reminiscent of an oligodendrocyte. (B) Spinal cord white matter showed a “mature” lesion constituted by amorphous and acellular material engulfing many myelin fibres and cellular debris (<i>arrow</i>). The surrounding nerve fibres were more or less disturbed. Electron micrographs of oligodendrocyte modifications consisting of intracytoplasmic inclusions. (C) Electron micrographs of frontal section of mature demyelinating plaque. The major part of the centre of the lesion (<i>star</i>) is composed of membranous, vesicular structures and fibrillary elements, but no cytological organelles (e.g. mitochondria or endoplasmic reticulum) were identifiable and no membranous binding was observed. Immediately around the centre, there are many myelinic and amyelinic processes, some of which are degenerated. The last “ring” is composed of many surrounding cells (<i>white arrows</i>) and complete the white matter lesion. Some cells are easily recognizable as astrocytes, their cytoplasm containing gliofilament tangles; some others could be histologically reminiscent of oligodendrocyte cells while others are not recognizable in the absence of specific markers. (D) High magnification of the area marked by a star in (C) evidenced myelinic bodies, small vesicles intermingled with membranous processes and fibrillary and amorphous material. These ultrastructural features of lesions were similar irrespective of the studied brain region (cerebellum, spinal cord and internal capsules). Scale bar: (A) and (B) 40 μm, (C) 15 μm, (D) 1.5 μm.</p