Alignment of AetAOX (<i>A</i>. <i>tauschii</i>) proteins with TbAOX (<i>T</i>. <i>brucei</i>).

<p>Yellow highlights indicate conserved motifs. Red font indicates residues proposed to coordinate the diiron center of the active site. Blue font indicates residues experimentally tested for loss of activity by previous researchers. Underlined residues are involved in the TbAOX hydrophobic cavity.</p

Transmembrane topologies of wheat AOX proteins predicted by Phyre2.

<p>Transmembrane topologies of wheat AOX proteins predicted by Phyre2.</p

Heat map of expression profiles for high-confidence <i>TaAOX</i> genes under biotic and abiotic stresses.

<p>Heat map of expression profiles for high-confidence <i>TaAOX</i> genes under biotic and abiotic stresses.</p

Classification of wheat AOX proteins using Arabidopsis AOX1a as reference.

<p>Classification of wheat AOX proteins using Arabidopsis AOX1a as reference.</p

Alignment of TuAOX (<i>T</i>. <i>urartu</i>) proteins with TbAOX (<i>T</i>. <i>brucei</i>).

<p>Yellow highlights indicate conserved motifs. Red font indicates residues proposed to coordinate the diiron center of the active site. Blue font indicates residues experimentally tested for loss of activity by previous researchers. Underlined residues are involved in the TbAOX hydrophobic cavity.</p

Occurrence of conserved motifs for known positive and negative AOX regulators in the promoters of the <i>TaAOX</i> gene family.

<p>Occurrence of conserved motifs for known positive and negative AOX regulators in the promoters of the <i>TaAOX</i> gene family.</p

Alignment of select TaAOX (hexaploid wheat) proteins with TbAOX (<i>T</i>. <i>brucei</i>).

<p>Yellow highlights indicate conserved motifs. Red font indicates residues proposed to coordinate the diiron center of the active site. Blue font indicates residues experimentally tested for loss of activity by previous researchers. Underlined residues are involved in the TbAOX hydrophobic cavity. Splice variants were identical for the protein region analyzed. The “reg” proteins were not analyzed due to the absence of the conserved motifs.</p

Putative cis-elements for hormonal and developmental responses in promoter regions.

<p>(A) <i>TaAOX</i> gene family and (B) diploid <i>AOX</i> gene families. The promoter for <i>put</i>.<i>regTaAOX-6BL</i> was not analyzed due to the majority of the promoter sequence being unresolved.</p

Heat map of expression profiles for high-confidence <i>TaAOX</i> genes at different developmental stages.

<p>Heat map of expression profiles for high-confidence <i>TaAOX</i> genes at different developmental stages.</p

Splice variants of hexaploid wheat <i>AOX</i> genes.

<p>Splice variants of hexaploid wheat <i>AOX</i> genes.</p