MOESM1 of Transcriptome analysis of Plasmodium berghei during exo-erythrocytic development

Additional file 1: Table S1. RNA-seq data of different life cycle stages of P. berghei used in this study. Table S2. RNA-seq analysis: Raw sequencing counts per gene in the different life cycle stages. Table S3. Gene ontology (GO) term annotation of genes of the individual communities in the GCN. Table S4. P. berghei geneIDs of genes of the different communities in the GCN. Table S5. Genes preferentially expressed in developing EEF stages compared to developing EF stages. Table S6. Genes preferentially expressed in detached cells (DCs/merosomes) compared to erythrocytic schizonts. Table S7. Genes preferentially expressed in blood schizonts (22 h) compared to EEF stages. Table S8. Genes preferentially expressed in blood schizonts (22 h) compared to all other stages. Table S9. Genes preferentially expressed in detached cells (DCs/merosomes) compared to all other stages

MOESM2 of Transcriptome analysis of Plasmodium berghei during exo-erythrocytic development

Additional file 2: Figure S1. Generation and genotyping of parasites expressing gfp under control of the promoter of PBANKA_1003900 (PBANKA_1003900GFP). Figure S2. Fluorescence-activated cell sorting of infected HeLa cells preserved in RNAlater. Figure S3. RNA expression profiles of 3 housekeeping genes (gapdh, actinI, tubulin1). Figure S4. RNA expression profiles of 5 genes encoding serine-repeat antigens, serine-type proteases (SERA1-5). Figure S5. RNA expression profiles of 5 genes encoding proteins of the parasitophorous vacuole membrane (Exported protein 1, Exported protein 2, UIS3, UIS4). Figure S6. RNA expression profiles of genes encoding 2 sporozoite surface proteins (CSP and TRAP). Figure S7. RNA expression profiles of 4 genes encoding enzymes involved in fatty acid biosynthesis (FabB/F, FabI, FabZ, FabG). Figure S8. RNA expression profiles of 6 genes encoding merozoite surface proteins (MSP). Figure S9. RNA expression profiles of 3 genes whose promoter regions have been used to drive expression of fluorescent/luminescent reporter proteins (HSP70, two genes for EF1Îą)

MOESM3 of Transcriptome analysis of Plasmodium berghei during exo-erythrocytic development

Additional file 3. Time laps: GFP expression under the control of PBANKA_1003900 promoter during EEF stage development

A multistage antimalarial targets the plasmepsins IX and X essential for invasion and egress

Regulated exocytosis by secretory organelles is important for malaria parasite invasion and egress. Many parasite effector proteins, including perforins, adhesins, and proteases, are extensively proteolytically processed both pre- and postexocytosis. Here we report the multistage antiplasmodial activity of the aspartic protease inhibitor hydroxyl-ethyl-amine-based scaffold compound 49c. This scaffold inhibits the preexocytosis processing of several secreted rhoptry and microneme proteins by targeting the corresponding maturases plasmepsins IX (PMIX) and X (PMX), respectively. Conditional excision of PMIX revealed its crucial role in invasion, and recombinantly active PMIX and PMX cleave egress and invasion factors in a 49c-sensitive manner