Fertility is not altered in Oo<i>Rptor</i>^−/− females.

<p>Fertility curve comparing the average cumulative number of pups per Oo<i>Rptor</i>^−/− (red line) and Oo<i>Rptor</i>^+/+ (black line) female. All Oo<i>Rptor</i>^−/− females are fertile indicating that loss of mTORC1 from oocytes does not affect the fertility of female mice.</p

Normal follicular development in Oo<i>Rptor</i>^−/− mice.

<p>Morphological analysis of ovaries from Oo<i>Rptor</i>^−/− and Oo<i>Rptor</i>^+/+ littermates at PD35 and at 16 weeks of age. Ovaries from Oo<i>Rptor</i>^−/− and Oo<i>Rptor</i>^+/+ mice were embedded in paraffin, and sections 8 µm thick were prepared and stained with hematoxylin. The overall development of follicles (arrows) and corpora lutea (CL) in Oo<i>Rptor</i>^−/− mice was found to be normal (B, D, F, and H) compared to Oo<i>Rptor</i>^+/+ mice (A, C, E, and G).</p

PI3K–Akt signaling in Oo<i>Rptor</i>^−/− and Oo<i>Rptor</i>^+/+ oocytes.

<p>Oocytes were isolated from the ovaries of Oo<i>Rptor</i>^−/− and Oo<i>Rptor</i>^+/+ mice at PD12–14, and western blots were performed as described in the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0110491#s4" target="_blank">Materials and Methods</a>. Levels of phosphorylation of Akt at S473 and T308 are elevated in Oo<i>Rptor</i>^−/− oocytes compared to Oo<i>Rptor</i>^+/+ oocytes, and this indicates that PI3K–Akt signaling in Oo<i>Rptor</i>^−/− oocytes is enhanced. Levels of total Akt and β-actin were used as internal controls.</p