Haemodynamic and vascular parameters.

<p>* indicates variables analysed by Mann-Whitney U test.</p

Cardiac mass, structure and function parameters.

<p>* indicates variable analysed by Mann-Whitney U test.</p

Statistical analyses of systolic blood pressure and vascular parameters.

<p>Statistical analyses of systolic blood pressure and vascular parameters.</p

Figure 1

<p>A–B. Scout cardiovascular magnetic resonance image of thoracic aorta demonstrating the planning of a transverse section through proximal descending aorta at the level of the right pulmonary artery. <b>C.</b> Arterial stiffness equations. Area(s) = systolic area, area(d) = diastolic area, ΔP = SBP-DBP, ρ = blood density (1059 kg.m⁻³).</p

Statistical analyses of cardiac mass.

<p>Statistical analyses of cardiac mass.</p

Serum β2GPI levels after cardiac IRI. Serum total β2GPI levels in mice 24 h after cardiac IRI.

<p>NT = No treatment. rhβ2GPI = recombinant human β2GPI. Domain V = recombinant human Domain V. Horizontal line for each group represents mean.</p

Anti Gr-1 staining.

<p>(A) Treatment with rhDomain V prior to IRI resulted in less Gr-1 positive cell infiltration compared with control. (B) Representative images from within the area of risk showing immunolabelling for anti-Gr-1 in a mouse treated with sham LAD occlusion, (C) control and (D) rhDomain V. Images obtained using Aperio Scanscope XT digital scanner at 10x magnification.</p

β2GPI deficient mice and cardiac IRI.

<p>β2GPI -/- mice treated with control sustained the same myocardial infarction size on 1% TTC staining as WT mice. Domain V (n = 6) protects β2GPI -/- mice from cardiac IRI compared with control (n = 11). Reconstitution with rmβ2GPI (n = 6) resulted in similar infarction size to control mice. Individual points represent infarct size as a % of AAR of a single mouse. The horizontal line for each study group represents the mean. NS = not significant.</p

Domain V protects from cardiac IRI in Rag-1 -/- antibody deficient mice reconstituted with natural IgM antibodies.

<p>(A) Rag-1 -/- mice were treated with saline or pooled murine IgM prior to IRI. The mice treated with IgM were further treated with either saline or rhDomain V at the time of reperfusion. Domain V reduced Troponin I levels at 24 h to similar levels to the antibody deficient mice. (B) Treatment with rhDomain V reduced infarction size (as percentage of total LV) in antibody deficient mice reconstituted with pooled IgM. IgM = murine immunoglobulin M. Individual points represent infarct size as a % of LV of a single mouse. The horizontal line for each study group represents the mean.</p