3 research outputs found

    <i>In vivo</i> migratory properties of CD4<sup>+</sup>CD25<sup>+</sup> cells from the thymus of chicks at D0.

    No full text
    <p>CD4<sup>+</sup>CD25<sup>+</sup> or CD4<sup>+</sup>CD25<sup>−</sup> cells were collected from the thymus of zero-day-old (D0) chicks, labeled with carboxyfluorescein succinimidyl ester (CFSE), and injected into MHC-compatible chicks. At d 2, 5, and 10 post-injection, the spleen, cecal tonsils (CT), and lungs were analyzed for CFSE<sup>+</sup> cells. a–c, Means (± SD) without a common superscript differ significantly within an organ (<i>P</i><0.05). * indicates significant differences (<i>P</i><0.05) between CFSE<sup>+</sup> cells in the CD4<sup>+</sup>CD25<sup>+</sup> and CD4<sup>+</sup>CD25<sup>−</sup> cells injected groups on a particular day. n = 3.</p

    Percentage of CD4<sup>+</sup>CD25<sup>+</sup> cells in different organs of developing embryos and post-hatch chicks.

    No full text
    <p>Fertile eggs were collected and incubated. At d 15 (E15), 17 (E17), and 20 (E20) of incubation and d 0 (D0), 1 (D1), 2 (D2), and 6 (D6) post-hatch, the thymic lobes, spleens and cecal tonsils were collected and analyzed for CD4<sup>+</sup> and CD4<sup>+</sup>CD25<sup>+</sup> cells. CD4<sup>+</sup>CD25<sup>+</sup> cells were expressed either as a percentage of (A) CD4<sup>+</sup> cells or (B) as a percentage of all mononuclear cells in the sample. a–c, Means (± SD) without a common superscript differ significantly in thymus (<i>P</i><0.05). d–e, Means (± SD) without a common superscript differ significantly in spleen (<i>P</i><0.05). x–z, Means (± SD) without a common superscript differ significantly in cecal tonsils (<i>P</i><0.05). n = 3.</p

    Suppressive properties of CD4<sup>+</sup>CD25<sup>+</sup> cells from cecal tonsils of chicks at D1.

    No full text
    <p>Percentage of non-proliferating responder cells in proliferation suppression assay. CD4<sup>+</sup>CD25<sup>−</sup> responder cells were labeled with carboxyfluorescein succinimidyl ester (CFSE), treated with anti-CD3/CD28, and mixed with effector CD4<sup>+</sup>CD25<sup>+</sup> or CD4<sup>+</sup>CD25<sup>−</sup> cells collected from cecal tonsils of one-day-old chicks at an effector∶responder cell ratio of 1∶1 or 0∶1 (control). At 72 h of co-culture CFSE dilution of CFSE-labeled responder cells was analyzed to calculate non-proliferating cell percentage. Means (+ SD) without a common superscript differ significantly (<i>P</i><0.05). n = 3.</p
    corecore