Excoriated lesion on the left foot of a 9-year-old boy, before treatment.

<p>Excoriated lesion on the left foot of a 9-year-old boy, before treatment.</p

Orlistat treatment leads to altered morphology of <i>G. duodenalis</i> trophozoites.

<p>To analyse trophozoite morphology WB-C6 trophozoites were incubated with increasing orlistat concentrations for 24 h and stained with a Romanowsky stain (DiffQuick). Median body (black triangle); Flagella (triangle); Nucleus (N). A control, B 3.1 µM, C 6.3 µM and D 25 µM orlistat. Scale bar = 10µm.</p

Grossly inflamed and superinfected larval track on the thigh of a 8-year-old boy.

<p>Grossly inflamed and superinfected larval track on the thigh of a 8-year-old boy.</p

Excoriated lesion on the left foot of a 9-year-old boy, four weeks after treatment.

<p>Excoriated lesion on the left foot of a 9-year-old boy, four weeks after treatment.</p

Killing of <i>G. duodenalis</i> trophozoites by orlistat.

<p>Giardiacidal effects of orlistat or MTZ were determined for drug concentrations indicated using <i>G. duodenalis</i> strains WB-C6 and 14-03/F7. WB-C6 trophozoites (5×10⁴) and 14-03/F7 trophozoites (1×10⁵) were treated with orlistat and MTZ, respectively, for 24 h and, after drug removal, cultured for another 48 h. Trophozoite numbers were determined every 24 h. (A) Orlistat was used at 5×(21.5 µM), 10×(43 µM), and 15×(64.5 µM) of the IC50_24h value for WB-C6. (B) Similarly, MTZ was used at 5×(55 µM), 10×(110 µM), and 15×(165 µM) of the IC50_24h concentration for WB-C6. (C) Orlistat was used at 5×(14 µM), 10×(28 µM), and 15×(42 µM) of the IC50_24h value for 14-03/F7. (D) Similarly, MTZ was used at 5×(31 µM), 10×(62 µM), and 15×(93 µM) of the IC50_24h concentration for 14-03/F7. Data are expressed as mean ± SD of parasite numbers determined in triplicate cultures. One of three experiments with similar results is shown (note the gap on the y-intercept).</p

Impairment of life quality in adult and child patients with CLM (n = 91).

a<p>only employed patients analyzed.</p>b<p>n = 87.</p>c<p>n = 7.</p

Clinical and demographic characteristics of patients with CLM (n = 91).

a<p>multiple classifications possible.</p>b<p>pustules, suppuration, abscess.</p>c<p>in case of multiple lesions, appearance of the oldest.</p

Correlation between the number of affected areas and impairment of skin disease-associated life quality (rho = 0.36; p = 0.004).

<p>Correlation between the number of affected areas and impairment of skin disease-associated life quality (rho = 0.36; p = 0.004).</p

Dermatology life quality impairment in patients with CLM (n = 91).

<p>Dermatology life quality impairment in patients with CLM (n = 91).</p

Categories of the modified Dermatology Life Quality Index.

<p>Categories of the modified Dermatology Life Quality Index.</p