MOESM2 of Ubiquitin-specific protease 22 is critical to in vivo angiogenesis, growth and metastasis of non-small cell lung cancer

Additional file 2. Differentially expressed gene list in USP22-Ko A549 and H1299 cells

MOESM1 of Ubiquitin-specific protease 22 is critical to in vivo angiogenesis, growth and metastasis of non-small cell lung cancer

Additional file 1 Fig. S1. Differentially expressed gene in USP22-Ko A549 and H1299 cells. Fig. S2. Impact of knockdown of USP22 on H2Bub1/methylatiom of H3K4/K79, cancer cell proliferation, and cell cycle progression. Fig. S3. Negative USP22 IHC stains in adjacent normal cells and tissues. Figure 4. Impact of knockout of USP22 on in vitro migration and invasion of A549 and H1299 cells. Fig. S5. Elevated USP22 in cancer cells survived cisplatin treatment. Fig. S6. Dynamics of P53 in A549 and USP22-Ko cells after 5 and 10 Gy irradiation

Additional file 2: of Targeting histone methyltransferase G9a inhibits growth and Wnt signaling pathway by epigenetically regulating HP1α and APC2 gene expression in non-small cell lung cancer

Figure S1. Inhibition of G9a induces cell cycle arrest. a A549 in G1 phase b H1299 in G2 phase. The statistical analyses of G1 and sub-G1 are shown (* P < 0.05 compared to control siRNA). Figure S2. Impact of knockdown of G9a on the levels of p53 and c-Myc proteins in A549 (p53 wild-type), H1299 (p53 null), and H1975 (p53 mutated) cancer cells. Figure S3. Impact of knockdown of HP1α on NSCLC cells proliferation. a Western blot of HP1α in three cell lines. b Cell proliferation after 72 h posttransfection of HP1α siRNA. (*P < 0.05, **P < 0.01, compared to cells transfected with control siRNA. Figure S4. Correlation between the level of G9a mRNA with that of DKK1 mRNA and WIF1 mRNA. The statistical analysis showed that the level of G9a mRNA level is reversely correlated with that of a DKK1 mRNA (P = 0.00828, R = - 0.38), b WIF1 mRNA (P = 0.0403, R = -0.3) in 46 of LUSC tissues. X-axis is for the normalized G9a (EMHT2) mRNA level, Y-axis is for the normalized DKK1/WIF1 mRNA level. Figure S5. Unsupervised hierarchical clustering analysis of selected upregulated genes upon G9a knockdown. These genes include  ICAM5, JPH3, MGMT, MMP2, RASSF1α etc. that are frequently silenced by epigenetic mechanisms in lung cancers. The two top rows represent two independent siRNA repeats, Ctrl1/2 is for control siRNA and G9a-KD1/2 is for G9A siRNA1/2. Figure S6. UNC0638 treatment decreased HP1α in H1299 cells. Cells were treated with defined UNC0638 for 72 hours and then protein was extracted for Western blot analysis of HP1 α, G9a, H3K9-Me2. (PDF 438 kb

Additional file 1: of Targeting histone methyltransferase G9a inhibits growth and Wnt signaling pathway by epigenetically regulating HP1Îą and APC2 gene expression in non-small cell lung cancer

Table S1. Differentially expressed genes in three G9a-attenuated lung cancer cells (DOCX 52 kb