362 research outputs found

    Application of LIBS in Detection of Antihyperglycemic Trace Elements in Momordica charantia

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    The present study exploits the information based on concentration of trace elements and minerals in understanding the role/mechanism of action of freeze-dried fruit powder suspended in distilled water of Momordica charantia (family: Cucurbitaceae) in diabetes treatment. Laser-induced break down spectroscopy (LIBS) spectra of plant product was recorded under optimized experimental conditions and analyzed. Several atomic lines such as Na, K, Mg, Ca, Fe, Al, etc. have been observed in the LIBS spectra of the above plant product. The concentrations of these minerals are determined by using calibration-free LIBS method. Correlation between the concentration of these elements/minerals and their defined role in diabetes management was studied in normal as well as diabetic animal model

    LIBS-Based Detection of Antioxidant Elements in Seeds of Emblica officinalis

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    The aim of the study was to determine the effect of the elements of the extract of seed from Emblica officinalis on antioxidant enzymes and osmotic fragility of erythrocytes membrane in normal as well as streptozotocin-induced severely diabetic albino Wister rats. The results revealed that the untreated diabetic rats exhibited increase in oxidative stress as indicated by significantly diminished activities of free radical scavenging enzymes such as catalase (CAT) and superoxide dismutase (SOD) by 37.5% (p

    Pattern of cutaneous wound healing in a live fish Clarias batrachus (L.)(Clariidae, Pisces.)

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    Incisional wounds of the same length and depth were made on skin between dorsal fin and the lateral line canal of Clarias batrachus and the pattern of wound closure has been studied histologically. Following infliction, a marked change in the colour of the skin surrounding the wound was observed which lasted for about 30 h and restored thereafter. Mucus and blood cells plugged the wound gap shortly after infliction. The epidermis surrounding the wound was found to be detached from the basement membrane. Mass movement of epidermal cells was observed from both side of the wound gap. The epidermal cells at the margin of the wound became hypertrophied. The epidermis became normal by 32 days. The dealing of sub-epidermal tissue indicated degenerative and regenerative changes of muscle fibres. The mucus and blood cells were accumulated in the wound gap and later fine blood vessels were formed. Gradually granulation tissue was formed and fibroblasts and myoblasts appeared. Myoblast differentiated into muscle bundles. The epidermal repair was completed within 35 days

    Utilisation of vegetable leaves for carp production

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    The results of two sets of experiments on mono-culture of grass carp (Ctenopharyngodon idella) and mixed culture of carps (grass carp 50 : catla 20 : rohu 15 : mrigal 15) fed exclusively with vegetable leaves are reported. The experiments were conducted with two replicates each in 0.02 ha ponds of Wastewater Aquaculture Division of the Central Institute of Freshwater Aquaculture, Rahara during 1991-93. Monoculture of grass carp stocked at 1000/ha demonstrated an average net production of 21.0 kg/ 0.02 ha/8 months (1501 kg/ha/yr). Mixed culture of carps stocked at 5000 /ha recorded an average net production of 22.5 kg/0.02 ha/8 months (1903.7 kg/ha/yr). Field studies revealed that water bind weed (Ipomoea aquatica) is the most preferred feed of grass carp amongst vegetable leaves followed by amaranths (Amaranthus gangeticus and Amaranthus viridis), cauliflower (Brassica oleracia var. votrytis) and cabbage (Brassica oleracia var. capitata) leaves. Through selection of highly productive leaf vegetables and suitable crop planning on fallow fish pond dykes, round the year feeding programme of grass carp has been explored. Recycling of sewage effluent for vegetable production and utilisation of vegetable leaves for fish production is considered an ideal way of integrated resource management for low cost production

    Development and evaluation of floating microspheres of curcumin in alloxan-induced diabetic rats

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    Purpose: To prepare and evaluate floating microspheres of curcumin for prolonged gastric residence and to study their effect on alloxan-induced diabetic rats.Methods: Floating microsphere were prepared by emulsion-solvent diffusion method, using hydroxylpropyl methylcellulose, chitosan and Eudragit S 100 polymer in varying proportions. Ethanol/dichloromethane blend was used as solvent in a ratio of 1:1. The floating microspheres were evaluated for flow properties, particle size, incorporation efficiency, as well as in-vitro floatability and drug release. The anti-diabetic activity of the floating microspheres of batch FM4 was performed on alloxaninduced diabetic rats.Result: The floating microspheres had particle size, buoyancy, drug entrapment efficiency and yield in the ranges of 255.32 - 365.65 μm, 75.58 - 89.59, 72.6 - 83.5, and 60.46 - 80.02 %, respectively. Maximum drug release after 24 h was 82.62 % for formulation FM4 and 73.879, 58.613 and 46.106 % for formulations FM1, FM2, and FM3 respectively. In-vivo data obtained over a 120-h period indicate that curcumin floating microspheres from batch FM4 showed the better glycemic control than control and a commercial brand of the drug.Conclusion: The developed floating curcumin delivery system seems economical and effective in diabetes management in rats, and enhances the bioavailability of the drug.Keywords: Gastro-retentive, Sustained release, Bioavailability, Curcumin, Floating microspheres, Diabete
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