Supplementary Figure S1 from <i>Paired Box 5</i> Methylation Detection by Droplet Digital PCR for Ultra-Sensitive Deep Surgical Margins Analysis of Head and Neck Squamous Cell Carcinoma

The distributions of relative QMSP values of PAX5 methylation are shown.</p

Supplementary Table S1, S2, S3, S4 from <i>Paired Box 5</i> Methylation Detection by Droplet Digital PCR for Ultra-Sensitive Deep Surgical Margins Analysis of Head and Neck Squamous Cell Carcinoma

Supplementary Table S1: List of primers and probes used for QMSP and ddQMSP Supplementary Table S2: Analysis of clinicopathological and molecular factors in association with clinical outcomes of imprint cases Supplementary Table S3: Analysis of clinicopathological and molecular factors in association with clinical outcomes of tissue cases Supplementary Table S4: QMSP assay of 6 HNSCC surgical margin tissues which were histologically cancer-positive</p

Prenatal exposure to tobacco smoke leads to increased mitochondrial DNA content in umbilical cord serum associated to reduced gestational age

<p>We investigated if prenatal exposures to tobacco smoke lead to changes in mitochondrial DNA content (mtDNA) in cord serum and adversely affect newborns’ health. Umbilical cord serum cotinine levels were used to determine in utero exposure to smoking. Cord serum mtDNA was measured by quantitative polymerase chain reaction analysis of the genes coding for cytochrome c oxidase1 (<i>MT</i>-<i>CO1</i>) and cytochrome c oxidase2 (<i>MT</i>-<i>CO2</i>). Log transformed levels of mtDNA coding for <i>MT</i>-<i>CO1</i> and <i>MT</i>-<i>CO2</i> were significantly higher among infants of active smokers with higher serum level of cotinine (<i>p</i> < 0.05) and inversely associated with gestational age (<i>p</i> = 0.08; <i>p</i> = 0.02). Structural equation modeling results confirmed a positive association between cotinine and <i>MT</i>-<i>CO1</i> and2 (<i>p</i> < 0.01) and inverse associations with gestational age (<i>p</i> = 0.02) and <i>IGF</i>-<i>1</i> (<i>p</i> < 0.01). We identified a dose-dependent increase in the level of <i>MT</i>-<i>CO1</i> and <i>MT</i>-<i>CO2</i> associated to increased cord serum cotinine and decreased gestational age.</p

Supplementary Table S2 from A Panel of Novel Detection and Prognostic Methylated DNA Markers in Primary Non–Small Cell Lung Cancer and Serum DNA

Supplementary Table S2.Thirty genes for further analyses selected from LUAD TCGA dataset.</p

Supplementary Table S1 from A Panel of Novel Detection and Prognostic Methylated DNA Markers in Primary Non–Small Cell Lung Cancer and Serum DNA

Supplementary Table S1.The PCR condition and sequences of the primer and the fluorescent probe used in this study.</p

Supplementary information from A Panel of Novel Detection and Prognostic Methylated DNA Markers in Primary Non–Small Cell Lung Cancer and Serum DNA

Supplementary information includes Supplementary Figure S1-S6 and Supplementary Materials and Methods.Figure S1 shows DMR scatterplots and bisulfite sequencing chromatrograms of 6 genes (CDO1, HOXA9, AJAP1, PTGDR, UNCX, and MARCH11). Figure S2 shows the correlation between methylation and the expression status of CDO1, PTGDR, AJAP1, and HOXA9 in the LUAD TCGA dataset. Figure S3 shows the Kaplan-Meier curves of overall survival according to the methylation status (positive or negative for hypermethylation) of tested genes in early stage disease (n=53). Figure S4 shows the methylation status of the 6 genes in serum samples from 43 stage IA LUAD, 40 stage IA LUSC, and 42 population-matched control subjects. Figure S5 shows promoter methylation levels for the 6 markers in pleural effusion and asities. Figure S6 shows the clinical significance of HOX paralogue group 9 genes (HOXA9, HOXB9, HOXC9, and HOXD9) in the TCGA dataset.</p

Supplementary Table S3 from A Panel of Novel Detection and Prognostic Methylated DNA Markers in Primary Non–Small Cell Lung Cancer and Serum DNA

Supplementary Table S3.The sensitivity and specificity of the 6 genes for cancer detection in body fluids.</p

Supplementary Table S4 from A Panel of Novel Detection and Prognostic Methylated DNA Markers in Primary Non–Small Cell Lung Cancer and Serum DNA

Supplementary Table S4.The correlation between clinicopathological features and methylation in lung cancer.</p

Supplementary Figure 2 from <i>HIST1H2BB</i> and <i>MAGI2</i> Methylation and Somatic Mutations as Precision Medicine Biomarkers for Diagnosis and Prognosis of High-grade Serous Ovarian Cancer

Supplementary Figure 2</p

Supplementary Figure 3 from <i>HIST1H2BB</i> and <i>MAGI2</i> Methylation and Somatic Mutations as Precision Medicine Biomarkers for Diagnosis and Prognosis of High-grade Serous Ovarian Cancer

Supplementary Figure 3</p