Additional file 1 of Assessment of the efficiency of synergistic photocatalysis on penicillin G biodegradation by whole cell Paracoccus sp

Additional file 1: Table S1. Degradation capability of Penicillin G by different strain species as reported from previous literatures and this study. Fig. S1a-c: Structure of the identified metabolites of peak Nos. 2 (a), 3 (a), 4 (b), and 5 (c) by LC–MS analysis. Peak Nos. 2 and 3 in HPLC: Potassium 2-(carboxy(2-phenylacetamido)methyl)-5,5-dimethylthiazolidine-4-carboxylate and potassium 2-(4-carboxy-5,5-dimethylthiazolidin-2-yl)-2-(2-phenylacetamido) acetate. Peak No. 4 in HPLC: Phenylacetic acid. Peak No. 5 in HPLC: Mixture of two isomers, 2-(amino(carboxyl)methyl)-5,5-dimethylthiazolidine-4-carboxylic acid. Fig. S2a-b: 1H NMR (a) and 13C NMR (b) spectra of potassium 2-(carboxy(2-phenylacetamido)methyl)-5,5-dimethylthiazolidine-4-carboxylate and potassium 2-(4-carboxy-5,5-dimethylthiazolidin-2-yl)-2-(2-phenylacetamido) acetate. 1H NMR (500 MHz, D2O) δ:7.34–7.45 (m, 10H); 5.07–5.06 (d, J = 3 Hz, 1H); 5.05–5.06 (d, J = 6 Hz, 1H); 4.79–4.78 (d, J = 3 Hz, 2H), 4.25–4.24 (d, J = 6 Hz, 1H); 3.81–3.80 (d, J = 4.0 Hz, 2H); 3.72–3.70 (d, J = 12 Hz, 1H); 3.43–3.42 (d, J = 3.5 Hz, 2H); 1.57 (s, 3H); 1.51 (s, 3H); 1.23 (s,3H); 1.05 (s, 3H).13C NMR (125 MHz, D2O) δ:176.17, 175.58, 175.24, 174.93, 174.86, 174.30, 135.01, 134.50, 129.65(2C), 129.49(2C), 129.27(2C), 129.04(2C), 127.61, 127.42, 75.84, 75.28, 67.00, 66.02, 60.01, 58.61, 58.45, 55.24, 42.51, 42.46, 27.98, 27.75, 26.75, 26.33. LC–MS (m/z): 391.3[M + 1]+ (cald. For C16H19KN2O5S, 390.1), 353.2[M` + 1]+ (cald for C16H20N2O5S, 352.1). Fig. S3a-b: 1H NMR (a) and 13C NMR (b) spectra of phenylacetic acid. 1H NMR (500 MHz, D2O) δppm: 7.42–7.32 (m, 5H); 3.73 (s, 2H).13CNMR (125 MHz, D2O) δppm: 177.01, 134.17, 129.38(2C), 128.79(2C), 127.27, 40.54. LC–MS (m/z): 137.3[M + 1]+ (cald. For C8H8O2, 136.05). Fig. S4a-b: 1H NMR (a) and 13C NMR (b) spectra of 5,5-dimethylthiazolidine. 1H NMR (500 MHz, D2O) δ: 5.06–5.05 (d, J = 4.5 Hz, 1H); 4.85–4.83 (d, J = 10.0 Hz, 1H); 3.97–3.96 (d, J = 4.0 Hz, 1H); 3.64–3.62 (d, J = 10.0 Hz, 1H); 3.56 (s, 2H); 3.47 (s, 1H); 3.19 (s,1H); 1.45 (s, 3H); 1.44 (s, 3H); 1.19 (s, 3H); 1.13(s, 3H). 13C NMR (125 MHz, D2O) δ: 176.34, 174.38, 171.85, 171.20, 74.47, 74.00, 63.59, 63.29, 59.31, 58.68, 56.80, 56.39, 29.29, 26.06, 26.02, 25.94. LC–MS (m/z): 235.1 [M + 1]+ (cald for C8H14N2O4S, 234.07)

Simultaneous production of propionic acid and vitamin B12 from corn stalk hydrolysates by <i>Propionibacterium freudenreichii</i> in an expanded bed adsorption bioreactor

Vitamin B12 and propionic acid that were simultaneous produced by Propionibacterium freudenreichii are both favorable chemicals widely used in food preservatives, medicine, and nutrition. While the carbon source and propionic acid accumulation reflected fermentation efficiency. In this study, using corn stalk as a carbon source and fed-batch fermentation process in an expanded bed adsorption bioreactor was studied for efficient and economic biosynthesis of acid vitamin B12 and propionic. With liquid hot water pretreated corn stalk hydrolysates as carbon source, 28.65 mg L−1 of vitamin B12 and 17.05 g L−1 of propionic acid were attained at 168 h in batch fermentation. In order to optimize the fermentation outcomes, fed-batch fermentation was performed with hydrolyzed corn stalk in expanded bed adsorption bioreactor (EBAB), giving 47.6 mg L−1 vitamin B12 and 91.4 g L−1 of propionic acid at 258 h, which correspond to product yields of 0.37 mg g−1 and 0.75 g g−1, respectively. The present study provided a promising strategy for economically sustainable production of vitamin B12 and propionic acid by P. freudenreichii fermentation using biomass cornstalk as carbon source and expanded bed adsorption bioreactor.</p