Two-way analysis of different factors.

<p>^a. Depths = 0–15 cm and 15–25 cm.</p><p>^b. Locations = upstream and downstream locations.</p><p>^c. Types = river wetland and constructed wetland.</p><p>Results with significant differences (<i>p</i> < 0.05) were shown in bold.</p><p>Two-way analysis of different factors.</p

The spatial distribution of dissolved organic carbon (DOC).

<p>Bar plots showing the spatial distribution of dissolved organic carbon (DOC) in the Xinxue River (XR) and Xinxue River Constructed Wetland (XRCW). A = upstream location of XRCW; B = downstream location of XRCW; C = upstream location of XR; D = downstream location of XR. Bars sharing the same lowercase letter (a or b) are not significant at α = 0.05 (Duncan Test).</p

The spatial distribution of OC types.

<p>The spatial distribution of OC types.</p

National, regional, and local maps of the study area.

<p>Maps showing the (a) national, (b) regional, and (c) local geographical setting of the study area. In map (c), showing the location of the Xinxue River (XR) and Xinxue River Constructed Wetland (XRCW): A = upstream location of XRCW; B = downstream location of XRCW; C = upstream location of XR; D = downstream location of XR.</p

The spatial and vertical distribution of heavy fraction organic carbon (HFOC).

<p>Bar plots showing the spatial and vertical (0–15 cm vs. 15–25 cm) distribution of heavy fraction organic carbon (HFOC) in the Xinxue River (XR) and Xinxue River Constructed Wetland (XRCW). A = upstream location of XRCW; B = downstream location of XRCW; C = upstream location of XR; D = downstream location of XR. Bars sharing the same letter are not significant at α = 0.05 (Duncan Test).</p

Two-way analysis of different factors.

<p>^a. Depths = 0–15 cm and 15–25 cm.</p><p>^b. Locations = upstream and downstream locations.</p><p>^c. Types = river wetland and constructed wetland.</p><p>Results with significant differences (<i>p</i> < 0.05) were shown in bold.</p><p>Two-way analysis of different factors.</p

The spatial distribution of light fraction organic carbon (LFOC).

<p>Bar plots showing the spatial distribution of light fraction organic carbon (LFOC) in the Xinxue River (XR) and Xinxue River Constructed Wetland (XRCW). A = upstream location of XRCW; B = downstream location of XRCW; C = upstream location of XR; D = downstream location of XR. Bars sharing the same lowercase letter (a or b) are not significant at α = 0.05 (Duncan test).</p

Pearson correlation analysis among the factors in Xinxue River Constructed Wetland.

<p>** Correlation is significant at the 0.01 level (2-tailed).</p><p>* Correlation is significant at the 0.05 level (2-tailed).</p><p>Pearson correlation analysis among the factors in Xinxue River Constructed Wetland.</p

The microfluidic assay flow.

<p>(A) Image of two microfluidic chips with attached thin film heaters and the two-barrel syringe pump. Glass syringes were connected to each chip with flexible tubing to load reagents and samples. Three ports were glued at the inlets of SPE channel and the waste port, and the outlet of the PCR channel. (B) Channel design with three sections: sample preparation (SPE), RT chamber and continuous flow PCR channel. Two fixed resistance heaters are attached via thermal tape to the bottom of the chip. Fluid flow between the channels was linear, and changes in fluid resistance allowed for valveless operation. The depth is 500 µm for SPE and RT channels, and the PCR channel is 100 µm deep. The widths are 500 µm for the SPE column, 1 mm for the RT chamber, and vary from 200 to 400 µm for the wide and narrow sections of the continuous flow PCR channel. The chip is 70 mm in length, 25 mm in width and 1.4 mm in height. (C) Microfluidic assay process flow. The nasopharyngeal sample is mixed with lysis buffer, applied to the chip, the chip is run, and the PCR products are read using a commercial capillary electrophoresis chip.</p

On-chip PCR product concentration vs. copy number showing NP swab and NP aspirate specimens.

<p>On-chip PCR product concentration vs. copy number showing NP swab and NP aspirate specimens.</p