Characterization of a novel non-specific nuclease from thermophilic bacteriophage GBSV1-7

, induced; 3, recombinant bacterium (containing gene), non-induced; 4, recombinant bacterium (containing gene), induced; 5, purified GST-GBSV1-NSN fusion protein; 6, purified GBSV1-NSN.<p><b>Copyright information:</b></p><p>Taken from "Characterization of a novel non-specific nuclease from thermophilic bacteriophage GBSV1"</p><p>http://www.biomedcentral.com/1472-6750/8/43</p><p>BMC Biotechnology 2008;8():43-43.</p><p>Published online 28 Apr 2008</p><p>PMCID:PMC2390534.</p><p></p

Characterization of a novel non-specific nuclease from thermophilic bacteriophage GBSV1-0

Da Ba01 [GenBank: ], phage SPP1 [GenBank: ], phage 370.3 [GenBank: ], phage EJ-1 [GenBank: ] and phage phiC2 [GenBank: ]. The DnaD-like domain of GBSV1-NSN held positions 158–227.<p><b>Copyright information:</b></p><p>Taken from "Characterization of a novel non-specific nuclease from thermophilic bacteriophage GBSV1"</p><p>http://www.biomedcentral.com/1472-6750/8/43</p><p>BMC Biotechnology 2008;8():43-43.</p><p>Published online 28 Apr 2008</p><p>PMCID:PMC2390534.</p><p></p

Characterization of a novel non-specific nuclease from thermophilic bacteriophage GBSV1-1

, induced; 3, recombinant bacterium (containing gene), non-induced; 4, recombinant bacterium (containing gene), induced; 5, purified GST-GBSV1-NSN fusion protein; 6, purified GBSV1-NSN.<p><b>Copyright information:</b></p><p>Taken from "Characterization of a novel non-specific nuclease from thermophilic bacteriophage GBSV1"</p><p>http://www.biomedcentral.com/1472-6750/8/43</p><p>BMC Biotechnology 2008;8():43-43.</p><p>Published online 28 Apr 2008</p><p>PMCID:PMC2390534.</p><p></p

Characterization of a novel non-specific nuclease from thermophilic bacteriophage GBSV1-4

°C for 15 min. For pH stability experiments, the GBSV1-NSN was pre-incubated in a series of pH buffers (pH 5.0 to 10.0) for 15, 30, 60, 90, 120, 150 or 180 min before assay. Each reaction mixture consisted of 100 μg/ml herring dsDNA and 295 μg/ml of the purified GBSV1-NSN protein. Each point represented the mean of triplicate assays and the error bars indicated the standard deviations.<p><b>Copyright information:</b></p><p>Taken from "Characterization of a novel non-specific nuclease from thermophilic bacteriophage GBSV1"</p><p>http://www.biomedcentral.com/1472-6750/8/43</p><p>BMC Biotechnology 2008;8():43-43.</p><p>Published online 28 Apr 2008</p><p>PMCID:PMC2390534.</p><p></p

Characterization of a novel non-specific nuclease from thermophilic bacteriophage GBSV1-5

S. The nucleic acid concentrations were varied in the range of 0–100 μg/ml. All nuclease activity assays were conducted at 60°C and pH 7.5. Each point represented the mean of triplicate assays and the error bars represented the standard deviations.<p><b>Copyright information:</b></p><p>Taken from "Characterization of a novel non-specific nuclease from thermophilic bacteriophage GBSV1"</p><p>http://www.biomedcentral.com/1472-6750/8/43</p><p>BMC Biotechnology 2008;8():43-43.</p><p>Published online 28 Apr 2008</p><p>PMCID:PMC2390534.</p><p></p

Characterization of a novel non-specific nuclease from thermophilic bacteriophage GBSV1-6

Da Ba01 [GenBank: ], phage SPP1 [GenBank: ], phage 370.3 [GenBank: ], phage EJ-1 [GenBank: ] and phage phiC2 [GenBank: ]. The DnaD-like domain of GBSV1-NSN held positions 158–227.<p><b>Copyright information:</b></p><p>Taken from "Characterization of a novel non-specific nuclease from thermophilic bacteriophage GBSV1"</p><p>http://www.biomedcentral.com/1472-6750/8/43</p><p>BMC Biotechnology 2008;8():43-43.</p><p>Published online 28 Apr 2008</p><p>PMCID:PMC2390534.</p><p></p

Characterization of a novel non-specific nuclease from thermophilic bacteriophage GBSV1-8

Se I (B, C and D) was also included as positive control. The protein solutions were shown on the top and the nucleic acids were indicated on the right. 1 μg of nucleic acids were respectively incubated with 1.5 μg of the purified GBSV1-NSN protein in 20 μl of reaction buffer at 37°C for six hours.<p><b>Copyright information:</b></p><p>Taken from "Characterization of a novel non-specific nuclease from thermophilic bacteriophage GBSV1"</p><p>http://www.biomedcentral.com/1472-6750/8/43</p><p>BMC Biotechnology 2008;8():43-43.</p><p>Published online 28 Apr 2008</p><p>PMCID:PMC2390534.</p><p></p

Accurate and fast path computation on large urban road networks: A general approach

<div><p>Accurate and fast path computation is essential for applications such as onboard navigation systems and traffic network routing. While a number of heuristic algorithms have been developed in the past few years for faster path queries, the accuracy of them are always far below satisfying. In this paper, we first develop an agglomerative graph partitioning method for generating high balanced traverse distance partitions, and we constitute a three-level graph model based on the graph partition scheme for structuring the urban road network. Then, we propose a new hierarchical path computation algorithm, which benefits from the hierarchical graph model and utilizes a region pruning strategy to significantly reduce the search space without compromising the accuracy. Finally, we present a detailed experimental evaluation on the real urban road network of New York City, and the experimental results demonstrate the effectiveness of the proposed approach to generate optimal fast paths and to facilitate real-time routing applications.</p></div

Computational times and accuracy comparisons of various algorithms on Schemes 1–4.

<p>Computational times and accuracy comparisons of various algorithms on Schemes 1–4.</p

Computational costs comparison of various algorithms on Schemes 1–4.

<p>(a) Average execution time of HiARP for the five test sets. (b) Average number of regions pruned during the search of HiARP. (c) Average execution time of HIPLA for the five test sets. (d) Average execution time of hierarchical Dijkstra algorithm for the five test sets.</p