Optimal control for Hamiltonian parameter estimation in non-commuting and bipartite quantum dynamics

The ability to characterise a Hamiltonian with high precision is crucial for the implementation of quantum technologies. In addition to the well-developed approaches utilising optimal probe states and optimal measurements, the method of optimal control can be used to identify time-dependent pulses applied to the system to achieve higher precision in the estimation of Hamiltonian parameters, especially in the presence of noise. Here, we extend optimally controlled estimation schemes for single qubits to non-commuting dynamics as well as two interacting qubits, demonstrating improvements in terms of maximal precision, time-stability, as well as robustness over uncontrolled protocols.Comment: Submission to SciPost Physics; 18 pages, 13 figure

Isolation and molecular identification of endophytic diazotrophs from seeds and stems of three cereal crops.

Ten strains of endophytic diazotroph were isolated and identified from the plants collected from three different agricultural crop species, wheat, rice and maize, using the nitrogen-free selective isolation conditions. The nitrogen-fixing ability of endophytic diazotroph was verified by the nifH-PCR assay that showed positive nitrogen fixation ability. These identified strains were classified by 879F-RAPD and 16S rRNA sequence analysis. RAPD analyses revealed that the 10 strains were clustered into seven 879F-RAPD groups, suggesting a clonal origin. 16S rRNA sequencing analyses allowed the assignment of the 10 strains to known groups of nitrogen-fixing bacteria, including organisms from the genera Paenibacillus, Enterobacter, Klebsiella and Pantoea. These representative genus are not endophytic diazotrophs in the conventional sense. They may have obtained nitrogen fixation ability through lateral gene transfer, however, the evolutionary forces of lateral gene transfer are not well known. Molecular identification results from 16S rRNA analyses were also confirmed by morphological and biochemical data. The test strains SH6A and MZB showed positive effect on the growth of plants

Depletion of butyrate-producing microbes of the Firmicutes predicts nonresponse to FMT therapy in patients with recurrent Clostridium difficile infection

ABSTRACTApproximately 10% of individuals diagnosed with Clostridium difficile infection (CDI) show the resistance to fecal microbiota transplantation (FMT), with the underlying mechanisms remaining elusive. Deciphering the intricate microbiome profile within this particular subset of FMT-refractory patients via clinical FMT investigations assumes paramount importance, as it holds the key to designing targeted therapeutic interventions tailored for CDI, particularly recurrent CDI (rCDI). A cohort of twenty-three patients afflicted with rCDI, exhibiting congruent clinical baselines, was meticulously selected for FMT. Rigorous screening of thousands of healthy individuals identified ten FMT donors who met stringent health standards, while a total of 171 stool samples were collected to serve as healthy controls. To assess the influence of microbiome dynamics on FMT efficacy, fecal samples were collected from four donors over a continuous period of twenty-five weeks. After FMT treatment, seven individuals exhibited an inadequate response to FMT. These non-remission patients displayed a significant reduction in α-diversity indexes. Meanwhile, prior to FMT, the abundance of key butyrate-producing Firmicutes bacteria, including Christensenellaceae_R_7_group, Ruminococcaceae_unclassified, Coprococcus_2, Fusicatenibacter, Oscillospira, and Roseburia, were depleted in non-remission patients. Moreover, Burkholderiales_unclassified, Coprococcus_2, and Oscillospira failed to colonize non-remission patients both pre- and post-treatment. Conversely, patients with a favorable FMT response exhibited a higher relative abundance of Veillonella prior to treatment, whereas its depletion was commonly observed in non-remission individuals. Genera interactions in lower effectiveness FMT donors were more similar to those in non-remission patients, and Burkholderiales_unclassified, Coprococcus_2, and Oscillospira were frequently depleted in these lower effectiveness donors. Older patients were not conducive to the colonization of Veillonella, consistent with their poor prognosis after FMT. FMT non-remission rCDI patients exhibited distinct characteristics that hindered the colonization of beneficial butyrate-producing Firmicutes microbes. These findings hold promise in advancing the precision of FMT therapy for rCDI patients

Characteristics of the isolates used in this study.

<p>Characteristics of the isolates used in this study.</p

Comparative analysis of 16S rRNA sequence from the RAPD strains SH6A, KHSA, GYA, B73A, KHB, GYB, MZA and representative strains from the GenBank.

<p>The significance of each branch is indicated by a bootstrap value calculated for 1000 subsets. Scalebar, 2 nt substitution per 100 nt.</p

(a) <i>nif</i>H gene fragments amplification (b) 879F-RAPD patterns, (c) 16S rRNA <i>Hin</i>fI-RFLP fingerprinting, (d) 16S rRNA <i>Msp</i>I-RFLP fingerprinting.

<p>DL2000 DNA marker (M), SH6A (lane 1), KHSA (lane 2), GYA (lane 3), ZY1A (lane 4), B73A (lane 5), KHB (lane 6), SH6B (lane 7), MZB (lane 8) and GYB (lane 9) and MZA (lane 10).</p