182 research outputs found

    Dopamine-Loaded Liposomes for in-Situ Amplified Photoelectrochemical Immunoassay of AFB<sub>1</sub> to Enhance Photocurrent of Mn<sup>2+</sup>-Doped Zn<sub>3</sub>(OH)<sub>2</sub>V<sub>2</sub>O<sub>7</sub> Nanobelts

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    A novel signal-amplified strategy based on dopamine-loaded liposome (DLL) was developed for competitive-type nonenzymatic photoelectrochemical (PEC) immunoassay of small- molecular aflatoxin B<sub>1</sub> (AFB<sub>1</sub>) in foodstuff, using Mn<sup>2+</sup>-doped Zn<sub>3</sub>(OH)<sub>2</sub>V<sub>2</sub>O<sub>7</sub>·2H<sub>2</sub>O nanobelts. The signal was amplified by high-loaded capacity of liposome and the highly efficient dopamine molecule to enhance photocurrent of Mn<sup>2+</sup>-doped Zn<sub>3</sub>(OH)<sub>2</sub>V<sub>2</sub>O<sub>7</sub>·2H<sub>2</sub>O nanobelts. The loaded dopamine in the liposome was used as an electron donor to scavenge the hole and inhibit the charge recombination. To design such an immunoassay system, a AFB<sub>1</sub>–bovine serum albumin (AFB<sub>1</sub>–BSA) conjugate was covalently bound with the multifunctional liposome via the cross-linkage glutaraldehyde, whereas monoclonal anti-AFB<sub>1</sub> antibody was labeled onto a magnetic bead by typical carbodiimide coupling. Upon addition of target AFB<sub>1</sub>, a competitive immunoreaction was carried out between the analyte and the AFB<sub>1</sub>–BSA–DLL for the conjugated antibody on the magnetic bead. Followed by magnetic separation, the carried DLL on the magnetic bead was lysed by using Triton X-100 to release the encapsulated dopamine. The as-produced dopamine (as an elector donor) increased the photocurrent of the Mn<sup>2+</sup>-doped Zn<sub>3</sub>(OH)<sub>2</sub>V<sub>2</sub>O<sub>7</sub>·2H<sub>2</sub>O nanobelts. The photocurrent depended on the as-released amount of the dopamine. The change in the photocurrent enhanced with the increasing AFB<sub>1</sub> concentration. Under the optimal conditions, Mn<sup>2+</sup>-doped Zn<sub>3</sub>(OH)<sub>2</sub>V<sub>2</sub>O<sub>7</sub>·2H<sub>2</sub>O nanobelts exhibited good photoelectrochemical responses for the detection of AFB<sub>1</sub> and allowed the detection of AFB<sub>1</sub> at a concentration as low as 0.3 pg mL<sup>–1</sup> within a linear range from 0.5 pg mL<sup>–1</sup> to 10 ng mL<sup>–1</sup>. Importantly, this system provided an ideal PEC immune sensing platform based on Mn<sup>2+</sup>-doped Zn<sub>3</sub>(OH)<sub>2</sub>V<sub>2</sub>O<sub>7</sub>·2H<sub>2</sub>O nanobelts and the high-loaded liposome for the detection of small molecules

    Table1_Impact of selective reporting of antimicrobial susceptibility testing report on clinicians’ prescribing behavior of antibiotics.DOCX

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    Background: Selective reporting has important value in antibiotic management. The purpose of this study was to explore the impact of AST selective reporting on prescribing behavior, so as to provide evidence for the implementation and improvement of selective reporting policies in microbiology laboratories at home and abroad.Methods: A cross-sectional study was conducted in a teaching tertiary hospital in China in July 2021. We designed selective reports and routine reports for urinary tract infections caused by Escherichia coli and lower respiratory tract infections caused by Pseudomonas aeruginosa. Questionnaires were conducted among participants by case vignettes, and 116 valid questionnaires were collected. The appropriateness rate of antibiotic prescription and the prescription rate of drug-resistant antibiotics, cephalosporins, fluoroquinolones, and carbapenems were calculated and compared between the selective reporting group and the routine reporting group in each case.Results: In most cases, we found that AST selective reporting could increase the appropriateness rate of antibiotic prescription (p Conclusion: AST selective reporting can help promote the appropriate use of antibiotics and reduce the use of broad-spectrum antibiotics. It is suggested to develop scientific and effective selective reporting practices and strengthen the two-way communication between clinicians and microbiology laboratories, thereby enabling microbiology laboratories to play a more important role in clinical antimicrobial management.</p

    Quantitative Raman Spectroscopic Measurements of CO<sub>2</sub> Solubility in NaCl Solution from (273.15 to 473.15) K at <i>p</i> = (10.0, 20.0, 30.0, and 40.0) MPa

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    The knowledge of high pressure phase behavior of the CO<sub>2</sub>–H<sub>2</sub>O–NaCl system in a wide <i>T</i>–<i>P</i>–<i>m</i><sub>NaCl</sub> range is of great interest in the injection of CO<sub>2</sub> to deep reservoir for storage or enhancement of oil recovery (EOR). The calculation of CO<sub>2</sub> solubility in brine is very important to predict the CO<sub>2</sub> storage capacity in saline aquifers. However, CO<sub>2</sub> solubility data at high salinity and high pressure are limited, and few thermodynamic models can accurately predict CO<sub>2</sub> solubility when salinity is higher than 4.5 mol/kg. In this study, a noninvasive technique, quantitative Raman spectroscopy, was used to investigate the high pressure equilibria of the CO<sub>2</sub>–H<sub>2</sub>O–NaCl system. A total of 180 solubility data points were obtained for carbon dioxide in (1, 3, and 5) mol/kg NaCl solutions from (273.15 to 473.15) K up to 40 MPa. New parameters were derived to improve the Duan-type solubility model, thus it can be applied in CO<sub>2</sub> sequestration and EOR to accurately calculate the solubility of CO<sub>2</sub> in NaCl aqueous solution up to 6 mol NaCl/kg H<sub>2</sub>O from (273 to 473) K, (3 to 60) MPa

    Image3_Preoperative lung immune prognostic index predicts survival in patients with pancreatic cancer undergoing radical resection.png

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    BackgroundLung immune prognostic index (LIPI), a combination of derived neutrophil-to-lymphocyte ratio (dNLR) and lactate dehydrogenase (LDH), is currently attracting considerable interest as a potential prognostic indicator in many malignancies. Our study aimed to investigate the prognostic value of preoperative LIPI in patients with pancreatic ductal adenocarcinoma (PDAC) undergoing radical resection.MethodsWe retrospectively reviewed PDAC patients treated with radical resection from February 2019 to April 2021 at Chinese People's Liberation Army (PLA) general hospital. Based on the cut-off value of dNLR and LDH identified by X-tile, patients were divided into LIPI good and LIPI intermediate/poor group. Kaplan-Meier curve and log-rank test were used to compare the recurrence-free survival (RFS) and overall survival (OS) of the two groups. Univariate and multivariate Cox regression was used to identify the independent prognostic value of LIPI. Subgroup analysis was performed to identify specific population benefited from radical resection.ResultsA total of 205 patients were included and the median RFS and OS was 10.8 and 24.3 months, respectively. Preoperative LIPI intermediate/poor was related to worse RFS and OS (p ConclusionsPreoperative LIPI intermediate/poor can be an indicator of poor prognosis in patients with PDAC undergoing radical resection. LIPI good could be an effective marker of benefit from adjuvant chemotherapy. Larger studies are warranted for further validation.</p

    Genome assembly and ORF prediction using total, clean and control reads of simulated data, respectively.

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    *<p>FPR: false positive rate of the genes predicted, using <i>S. cerevisiae</i> as reference.</p

    The Type II alveolar epithelial cells from the PQ group (×8000).

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    <p>The nuclei were collapsed and contained dense heterochromatin; the endoplasmic reticulum tended to be vesiculated, the basement membranes were absent or disrupted, the mitochondria was vacuolated and the number of lamellar bodies clearly decreased.</p

    The Type II alveolar epithelial cells in the bosentan group (×8000).

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    <p>The damage was ameliorated to some degree relative to the PQ group. Their nuclei membrane and the endoplasmic reticulum were integral and smooth, the mitochondrial cristae were limpid but still a litter disorder, vacuolation of the lamellar bodies were ameliorated, a few mature lamellar bodies were observed.</p

    Statistics of real NGS data as testing data for Parallel-QC.

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    <p>S1, S2: human saliva DNA samples.</p><p>A1, A2: in-house sequenced algae DNA samples.</p

    Dynamic change of ET-1 in the serum from the three groups (mean±SD, ng/L).

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    <p>Dynamic change of ET-1 in the serum from the three groups (mean±SD, ng/L).</p

    Dynamic change of TGF-β1 in the lungs of the three groups (mean±SD, ng/L).

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    <p>Dynamic change of TGF-β1 in the lungs of the three groups (mean±SD, ng/L).</p
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