Signs of the legal regulation of bodies supporting enforcement of economic and other courts in the Soviet period

У статті визначено характерні ознаки правового регулювання діяльності органів, що забезпечували виконання рішень арбітражних та інших судів у радянський період. Названо основні нормативні акти радянського періоду. Виділено характеристики, що можна буде використати при модернізації сучасного українського законодавства.Typical signs of legal regulation of the authorities activities, which ensured enforcement of decisions arbitration and other courts in the Soviet period, have been identified in the article. The basic regulations acts of the Soviet period were identified. Characteristics, that can be used in modernization Ukrainian modern legislation, were allocated

Synthesis, Characterization and Theoretical Calculations of a New Fluorescent Probe for Detection Cu²⁺

<p>A novel rhodamine-based probe (<b>RP1</b>) was synthesized and characterized as a colorimetric chemosensor with high selectivity and sensitivity in biological systems toward Cu²⁺ over other cations tested. <b>RP1</b> showed low detection limit for copper. The binding between <b>RP1</b> and Cu²⁺ was confirmed to be stoichiometric. The recognition process was reversible. We also performed the density functional theory (DFT) calculation to have more understanding of structure, molecular and electronic.</p

Chain mediation model of perceived stress on emotional response, physical response, and behavioral response through coping style and resilience.

Chain mediation model of perceived stress on emotional response, physical response, and behavioral response through coping style and resilience.</p

Correlation analysis between variables.

Correlation analysis between variables.</p

Recombinant Scorpine Produced Using SUMO Fusion Partner in <i>Escherichia coli</i> Has the Activities against Clinically Isolated Bacteria and Inhibits the <i>Plasmodium falciparum</i> Parasitemia <i>In Vitro</i>

<div><p>Scorpine, a small cationic peptide from the venom of Pandinus imperator, which has been shown to have anti-bacterial and anti-plasmodial activities, has potential important applications in the pharmaceutical industries. However, the isolation of scorpine from natural sources is inefficient and time-consuming. Here, we first report the expression and purification of recombinant scorpine in <i>Escherichia coli,</i> using small ubiquitin-related modifier (SUMO) fusion partner. The fusion protein was expressed in soluble form in <i>E. coli</i>, and expression was verified by SDS-PAGE and western blotting analysis. The fusion protein was purified to 90% purity by nickel–nitrilotriacetic acid (Ni²⁺–NTA) resin chromatography. After the SUMO-scorpine fusion protein was cleaved by the SUMO protease, the cleaved sample was reapplied to a Ni²⁺–NTA column. Tricine/SDS-PAGE gel results indicated that Scorpine had been purified successfully to more than 95% purity. The recombinantly expressed Scorpine showed anti-bacterial activity against two standard bacteria including <i>Staphylococcus aureus</i> ATCC 29213 and <i>Acinetobacter baumannii</i> ATCC 19606, and clinically isolated bacteria including <i>S. aureus</i> S, <i>S. aureus</i> R, <i>A. baumannii</i> S, and <i>A. baumannii</i> R. It also produced 100% reduction in <i>Plasmodium falciparum</i> parasitemia <i>in vitro</i>. Thus, the expression strategy presented in this study allowed convenient high yield and easy purification of recombinant Scorpine for pharmaceutical applications in the future.</p></div

Indirect analysis results.

Indirect analysis results.</p

S1 Dataset -

(XLSX)</p

A comparison of benzyl and 2-naphthylmethyl ethers as permanent hydroxyl protecting groups in the synthesis of α-galactoglycosphingolipids KRN7000 and PBS-57

<p>Due to the interest in the biological properties of marine derived α-galactoglycosphingolipids (α-GalGSLs), a lot of work has focused on the development of their synthesis. Here we conducted the direct comparison of benzyl and 2-naphthylmethyl (Nap) ethers as permanent hydroxyl protecting groups for the synthesis of KRN7000, as well as the practical synthesis of a biologically active α-GalGSLs PBS-57. This work further revealed the advantages of Nap ether over benzyl ether for permanent hydroxyl protection in the synthesis of α-GalGSLs.</p