16 research outputs found

    Early stages of somatic embryogenesis in root callus of grasspea (Lathyrus sativus L.)

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    Callus cultures from root explants of Lathyrus sativus L. ‘Derek’ were tested for their morphogenic capacity. Primary explants (fragments of roots) were cultivated on three induction media. We obtained three lines of callus tissue among which we identified two non-embryogenic lines and one embryogenic line. Callus originally cultivated on modified MS medium supplemented with 0.05 mg*L-1 picloram, formed embryo-like structures upon transfer to media containing 0.1 mg*L-1 picloram or 0.9 mg*L-12,4-D. Histological examinations confirmed embryogenity of obtained structures. Previous studies had revealed that, notwithstanding efficient callus induction and proliferation, its capacity to differentiate shoots or somatic embryos is limited. Consequently, rhizogenesis was only form of complete organogenesis obtained in our experiments. However attempts to develop the methods for indirect plant regeneration in L. sativus would allow creation of new genetic variations required to improvement of this species

    Działalność wrocławskiego Zakładu Historii Edukacji w latach 1999–2009

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    Działalność wrocławskiego Zakładu Historii Edukacji w latach 1999–200

    Callus Induction and Rhizogenesis in Lathyrus sativus L.

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    Grass pea (Lathyrus sativus L.) is a leguminous plant distinguished by great resistance to abiotic and biotic stresses that can become a valuable source of protein feed in the nearest future. However, neglected by breeders, it needs the improvement of some disadvantages. Biotechnological techniques, including in vitro culture, are new and modern tools supporting plant breeding. Unfortunately, grass pea belongs to grain legumes that are well-known for their recalcitrance to in vitro manipulation and consequently plant regeneration. The aim of the study was the evaluation of different factors influence on callus induction and proliferation, as well as cell differentiation and organogenesis. Callus culture were initiated from different explants of two Polish cultivars of grasspea on media supplemented with auxin and various cytokinins both on the light and in the dark. The rate of tissue proliferation was significantly increased in both tested cultivars by light. More than 400 mg of tissue from one explant was obtained after 12 weeks of culture. The most intensive increase in callus mass was noted for internode fragments of ‘Krab’ (380 mg/one explant) and root fragments of ‘Derek’ (361 mg/one explant). On the media with the addition of thidiazuron callus tissue grew better (852 mg/one explants) than on the media with zeatin and kinetin (56–164 mg/one explant). Formation of the roots was the only type of organogenesis observed during the study. In ‘Derek’ callus on LP medium rhizogenesis occurred the most frequently (38%). The roots regenerated from this callus was also the most numerous (1.6) and the longest (12.5 mm)

    Effect of Escitalopram on the Number of DCX-Positive Cells and NMUR2 Receptor Expression in the Rat Hippocampus under the Condition of NPSR Receptor Blockade

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    Neuropeptide S (NPS) is a multifunctional regulatory factor that exhibits a potent anxiolytic activity in animal models. However, there are no reports dealing with the potential molecular interactions between the activity of selective serotonin reuptake inhibitors (SSRIs) and NPS signaling, especially in the context of adult neurogenesis and the expression of noncanonical stress-related neuropeptides such as neuromedin U (NMU). The present work therefore focused on immunoexpression of neuromedin U receptor 2 (NMUR2) and doublecortin (DCX) in the rat hippocampus after acute treatment with escitalopram and in combination with selective neuropeptide S receptor (NPSR) blockade. Studies were carried out on adult, male Sprague-Dawley rats that were divided into five groups: animals injected with saline (control) and experimental individuals treated with escitalopram (at single dose 10 mg/kg daily), escitalopram + SHA-68, a selective NPSR antagonist (at single dose 40 mg/kg), SHA-68 alone, and corresponding vehicle control. All animals were sacrificed under halothane anaesthesia. The whole hippocampi were quickly excised, fixed, and finally sliced for general qualitative immunohistochemical assessment of the NPSR and NMUR2 expression. The number of immature neurons was enumerated using immunofluorescent detection of doublecortin (DCX) expression within the subgranular zone (SGZ). Acute escitalopram administration affects the number of DCX and NMUR2-expressing cells in the adult rat hippocampus. A decreased number of DCX-expressing neuroblasts after treatment with escitalopram was augmented by SHA-68 coadministration. Early pharmacological effects of escitalopram may be at least partly connected with local NPSR-related alterations of neuroblast maturation in the rat hippocampus. Escitalopram may affect neuropeptide and DCX-expression starting even from the first dose. Adult neurogenesis may be regulated via paracrine neuropeptide S and NMU-related signaling

    Być zdolnym – wspierać zdolnych

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    Praca recenzowana / peer-reviewed paperByć zdolnym – wspierać zdolnych to książka szczególna. Zawiera teksty napisane przez nauczycieli – uczestników studiów podyplomowych Praca z uczniem zdolnym. Stanowią oni pierwszą grupę specjalistów w zakresie rozpoznawania, opieki i wspierania uczniów zdolnych w województwie świętokrzyskim. Takie kadry są szczególnie potrzebne wobec zmian w systemie pracy ze zdolnymi, wdrażanych w szkołach i placówkach od roku szkolnego 2011/2012. Studia były realizowane w ramach projektu Benild – Doskonalenie Świętokrzyskich Kadr Oświaty, współfinansowanego ze środków Unii Europejskiej w ramach Europejskiego Funduszu Społecznego, na Uniwersytecie Jana Kochanowskiego w Kielcach. Celem studiów było nabywanie przez nauczycieli kompetencji w obszarze identyfikacji i wspierania uczniów zdolnych
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