Integrating Nursing Context And Technology Usage For English Speaking Empowerment

English for specific purposes (ESP) must be taught to meet particular needs of non-English language students (Hutchinson & Waters, 1987, p.21). It gives direct suggestion that learning process should be designed in such away not only to meet the learning objectives of the students but also to give the students meaningful and enjoyable learning experiences (Crawford, 2013, p.1 81). The condition becomes more challenging when the ESP teachers are only having English language background. It implies that learning context, which meets the students’ needs, should be carried out in learning process along with the suitable and enjoyable learning strategies where the students can feel the pleasure instead of pressure. By considering the three steps of ESP course designing suggested by Nitu (2002, p.154), this paper aims at proving the effectiveness of integrating the students’ learning contexts and the students’ pleasure to empower the students’ speaking performance in Health Science Faculty of Musi Charitas Catholic University Palembang. This study belongs to experimental research with pre- and post-design. By the end of the study, it was proven that integrating the nursing field context and the usage of technology in the classroom can help the students improve their English speaking skills. For evidential information, some videos of learning process are presente

Additional file 2: Figure S1. of Circular RNA expression profiles and features in human tissues: a study using RNA-seq data

The top 10 circRNAs expression of each sample in six human adult normal tissues. (JPEG 161 kb

Instantaneous Magnetically Assembled and Hydrophilic Photonic Crystals with Controlled Diffraction Colors

The time-consuming assembling process of the traditional photonic crystals (PCs) and non-water-dispersibility of the reported magnetic responsive PCs (MRPCs) have greatly limited the application especially in the biotechnological fields. Herein, the hydrophilic and size-controllable Fe₃O₄@­poly­(4-styrenesulfonic acid-<i>co</i>-maleic acid) (PSSMA)­@­SiO₂ MRPCs were fabricated by orderly assembling of the core–shell colloidal nanocrystal clusters via a two-step facile synthesis approach. Due to the rich carboxyl and hydroxyl groups of PSSMA, the obtained MRPCs have excellent properties of hydrophilicity, high surface charge which presented magnetically tunable photonic structural colors, and rapid reflection signal in aqueous solution under external magnetic field within 1 s. The diffraction color of the MRPCs in the entire visible range could be tuned by adjusting the magnetic strength or the nanoparticle size, which bring a clear change of the structure color from brilliant red to modena by the naked eye. Thus, the magnetically sensitive MRPCs with low-cost, tunable size, and fast optical signal response indicate a promising application in optical systems, biosensors, and biomedical imaging

Tools for Investigation of the RNA Endonuclease Activity of Mammalian Argonaute2 Protein

Mammalian Argonaute2 (Ago2) protein is the key player of RNA-induced silencing complexes (RISCs), regulating gene function through RNA interference. In this paper, a method to investigate the RNA endonuclease activity of Ago2 is reported using electrochemical technique with G-quadruplex–hemin complexes as signal transduction probes. Experimental results reveal that Ago2 may exhibit its slicer activity without any biological partners or ATP in wide pH and temperature ranges; thus, a method to assay the activity of the enzyme is proposed. For purified samples, the endonuclease activity of Ago2 can be quantified in the range from 6.25 to 25 nM with a detection limit of 5.02 nM. In the case of porcine cardiocyte lysates which contain a certain amount of Ago2, a linear correlation can be also obtained between the electrochemical signal and the dilution radio of the lysates. The proposed method shows desirable sensitivity, high selectivity, and excellent reproducibility, implying that this method may hold considerable potential for functional studies of Ago2 and clinical diagnosis in the future

Instantaneous Magnetically Assembled and Hydrophilic Photonic Crystals with Controlled Diffraction Colors

The time-consuming assembling process of the traditional photonic crystals (PCs) and non-water-dispersibility of the reported magnetic responsive PCs (MRPCs) have greatly limited the application especially in the biotechnological fields. Herein, the hydrophilic and size-controllable Fe₃O₄@­poly­(4-styrenesulfonic acid-<i>co</i>-maleic acid) (PSSMA)­@­SiO₂ MRPCs were fabricated by orderly assembling of the core–shell colloidal nanocrystal clusters via a two-step facile synthesis approach. Due to the rich carboxyl and hydroxyl groups of PSSMA, the obtained MRPCs have excellent properties of hydrophilicity, high surface charge which presented magnetically tunable photonic structural colors, and rapid reflection signal in aqueous solution under external magnetic field within 1 s. The diffraction color of the MRPCs in the entire visible range could be tuned by adjusting the magnetic strength or the nanoparticle size, which bring a clear change of the structure color from brilliant red to modena by the naked eye. Thus, the magnetically sensitive MRPCs with low-cost, tunable size, and fast optical signal response indicate a promising application in optical systems, biosensors, and biomedical imaging

Table_3_Identification of endothelial-related molecular subtypes for bladder cancer patients.docx

BackgroundBladder cancer (BC) is a disease with significant heterogeneity and poor prognosis. The prognosis and therapeutic response of BC patients are significantly influenced by endothelial cells in the tumor microenvironment. In order to understand BC from the perspective of endothelial cells, we orchestrated molecular subtypes and identified key genes.MethodsSingle-cell and bulk RNA sequencing data were extracted from online databases. R and its relative packages were used to analyze these data. Cluster analysis, prognostic value analysis, function analysis, immune checkpoints, tumor immune environment and immune prediction were conducted.ResultsFive endothelial-related genes (CYTL1, FAM43A, HSPG2, RBP7, and TCF4) divided BC patients in the TCGA, GSE13507, and GSE32894 datasets into two clusters, respectively. In prognostic value analysis, patients in the cluster 2 were substantially associated with worse overall survival than those in the cluster 1 according to the results of TCGA, GSE13507 and GSE32894 datasets. In the results of functional analysis, the endothelial-related clusters was enriched in immune-related, endothelial-related and metabolism-related pathways. Samples in the cluster 1 had a statistically significant increase in CD4+ T cells and NK-cell infiltration. Cluster 1 was positively correlated with the cancer stem score and tumor mutational burden score. The results of immune prediction analysis indicated that 50.6% (119/235) of patients in the cluster 1 responded to immunotherapy, while the response rate in the cluster 2 decreased to 16.7% (26/155).ConclusionIn this study, we categorized and discovered distinctive prognosis-related molecular subtypes and key genes from the perspective of endothelial cells at the genetic level by integrating single-cell and bulk RNA sequencing data, primarily to provide a roadmap for precision medicine. </p

Ammonia Monooxygenase-Mediated Cometabolic Biotransformation and Hydroxylamine-Mediated Abiotic Transformation of Micropollutants in an AOB/NOB Coculture

Biotransformation of various micropollutants (MPs) has been found to be positively correlated with nitrification in activated sludge communities. To further elucidate the roles played by ammonia-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB), we investigated the biotransformation capabilities of an NOB pure culture (<i>Nitrobacter</i> sp.) and an AOB (<i>Nitrosomonas europaea</i>)/NOB (<i>Nitrobacter</i> sp.) coculture for 15 MPs, whose biotransformation was reported previously to be associated with nitrification. The NOB pure culture did not biotransform any investigated MP, whereas the AOB/NOB coculture was capable of biotransforming six MPs (i.e., asulam, bezafibrate, fenhexamid, furosemide, indomethacin, and rufinamide). Transformation products (TPs) were identified, and tentative structures were proposed. Inhibition studies with octyne, an ammonia monooxygenase (AMO) inhibitor, suggested that AMO was the responsible enzyme for MP transformation that occurred cometabolically. For the first time, hydroxylamine, a key intermediate of all aerobic ammonia oxidizers, was found to react with several MPs at concentrations typically occurring in AOB batch cultures. All of these MPs were also biotransformed by the AOB/NOB coculture. Moreover, the same asulam TPs were detected in both biotransformation and hydroxylamine-treated abiotic transformation experiments, whereas rufinamide TPs formed from biological transformation were not detected during hydroxylamine-mediated abiotic transformation, which was consistent with the inability of rufinamide abiotic transformation by hydroxylamine. Thus, in addition to cometabolism likely carried out by AMO, an abiotic transformation route indirectly mediated by AMO might also contribute to MP biotransformation by AOB

Image_2_A novel cuproptosis-related prognostic lncRNA signature for predicting immune and drug therapy response in hepatocellular carcinoma.tif

Intratumoral copper levels are closely associated with immune escape from diverse cancers. Cuproptosis-related lncRNAs (CRLs), however, have an unclear relationship with hepatocellular carcinoma (HCC). Gene expression data from 51 normal tissues and 373 liver cancer tissues from the Cancer Genome Atlas (TCGA) database were collected and analyzed. To identify CRLs, we employed differentially expressed protein-coding genes (DE-PCGs)/lncRNAs (DE-lncRNAs) analysis, Kaplan–Meier (K-M) analysis, and univariate regression. By univariate and Lasso Cox regression analyses, we screened 10 prognosis-related lncRNAs. Subsequently, five CRLs were identified by multivariable Cox regression analysis to construct the prognosis model. This feature is an independent prognostic indicator to forecast overall survival. According to Gene Set Variation Analysis (GSVA) and Gene Ontology (GO), both immune-related biological processes (BPS) and pathways have CRL participation. In addition, we found that the characteristics of CRLs were associated with the expression of the tumor microenvironment (TME) and crucial immune checkpoints. CRLs could predict the clinical response to immunotherapy based on the studies of tumor immune dysfunction and rejection (TIDE) analysis. Additionally, it was verified that tumor mutational burden survival and prognosis were greatly different between high-risk and low-risk groups. Finally, we screened potential sensitive drugs for HCC. In conclusion, this study provides insight into the TME status in patients with HCC and lays a basis for immunotherapy and the selection of sensitive drugs.</p

Table_2_Identification of endothelial-related molecular subtypes for bladder cancer patients.docx

BackgroundBladder cancer (BC) is a disease with significant heterogeneity and poor prognosis. The prognosis and therapeutic response of BC patients are significantly influenced by endothelial cells in the tumor microenvironment. In order to understand BC from the perspective of endothelial cells, we orchestrated molecular subtypes and identified key genes.MethodsSingle-cell and bulk RNA sequencing data were extracted from online databases. R and its relative packages were used to analyze these data. Cluster analysis, prognostic value analysis, function analysis, immune checkpoints, tumor immune environment and immune prediction were conducted.ResultsFive endothelial-related genes (CYTL1, FAM43A, HSPG2, RBP7, and TCF4) divided BC patients in the TCGA, GSE13507, and GSE32894 datasets into two clusters, respectively. In prognostic value analysis, patients in the cluster 2 were substantially associated with worse overall survival than those in the cluster 1 according to the results of TCGA, GSE13507 and GSE32894 datasets. In the results of functional analysis, the endothelial-related clusters was enriched in immune-related, endothelial-related and metabolism-related pathways. Samples in the cluster 1 had a statistically significant increase in CD4+ T cells and NK-cell infiltration. Cluster 1 was positively correlated with the cancer stem score and tumor mutational burden score. The results of immune prediction analysis indicated that 50.6% (119/235) of patients in the cluster 1 responded to immunotherapy, while the response rate in the cluster 2 decreased to 16.7% (26/155).ConclusionIn this study, we categorized and discovered distinctive prognosis-related molecular subtypes and key genes from the perspective of endothelial cells at the genetic level by integrating single-cell and bulk RNA sequencing data, primarily to provide a roadmap for precision medicine. </p

Table_1_A novel cuproptosis-related prognostic lncRNA signature for predicting immune and drug therapy response in hepatocellular carcinoma.xlsx

Intratumoral copper levels are closely associated with immune escape from diverse cancers. Cuproptosis-related lncRNAs (CRLs), however, have an unclear relationship with hepatocellular carcinoma (HCC). Gene expression data from 51 normal tissues and 373 liver cancer tissues from the Cancer Genome Atlas (TCGA) database were collected and analyzed. To identify CRLs, we employed differentially expressed protein-coding genes (DE-PCGs)/lncRNAs (DE-lncRNAs) analysis, Kaplan–Meier (K-M) analysis, and univariate regression. By univariate and Lasso Cox regression analyses, we screened 10 prognosis-related lncRNAs. Subsequently, five CRLs were identified by multivariable Cox regression analysis to construct the prognosis model. This feature is an independent prognostic indicator to forecast overall survival. According to Gene Set Variation Analysis (GSVA) and Gene Ontology (GO), both immune-related biological processes (BPS) and pathways have CRL participation. In addition, we found that the characteristics of CRLs were associated with the expression of the tumor microenvironment (TME) and crucial immune checkpoints. CRLs could predict the clinical response to immunotherapy based on the studies of tumor immune dysfunction and rejection (TIDE) analysis. Additionally, it was verified that tumor mutational burden survival and prognosis were greatly different between high-risk and low-risk groups. Finally, we screened potential sensitive drugs for HCC. In conclusion, this study provides insight into the TME status in patients with HCC and lays a basis for immunotherapy and the selection of sensitive drugs.</p