Additional file 1: Figure S1. of Hypoxia alters the recruitment of tropomyosins into the actin stress fibres of neuroblastoma cells

Hypoxic incubation of SH-EP neuroblastoma cells increases levels of hypoxia-inducible transcription factors, HIF-1α and HIF-2α. SH-EP cells were incubated for 0–144 h in normoxic (20 % O2) or hypoxic (1 % O2) conditions, before rapidly lysing cells on ice in RIPA buffer containing a protease inhibitor cocktail. Protein lysates were separated using SDS-PAGE and HIFs detected using anti-HIF-1α or anti-HIF-2α antibodies. Representative immunoblots, with HIF expression clearly upregulated in hypoxia (H) over normoxic controls (N). Cell lysates known to contain HIF-1α and HIF-2α used as positive control (+ve). n = 3. (PDF 1900 kb

Additional file 1 of ROR1 and ROR2 expression in pancreatic cancer

Additional file 1 : Supplementary Fig.S1. Correlation between mRNA and protein expression of ROR1 and ROR2 in CPTAC PDAC discovery cohort. A. ROR1 mRNA and protein expression was positively correlated (Pearson’s R=0.25, p=0.005). B. ROR2 mRNA and protein expression was positively correlated (Pearson’s R=0.48, p<0.001)