6 research outputs found

    Experimental configuration of single-molecule force spectroscopy assays.

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    <p>Scheme illustrating the measurement configuration that was used during the experiments with no force applied (A) and under force (B) when the stage was moving. C is a close-up of B.). The pilus attached to the trapped bead is disproportionally long for reasons of depiction. The 10.5-μm mounting bead (MB) was immobilized on the coverslip while the 1-μm probe bead (PB) was trapped by optical tweezers (OT). A piliated bacterium was non-specifically attached to the MB and a pilus to the PB. When the coverslip was moved, and the trap kept in a fixed position, a force was directly exerted on the pilus. (C) Assuming adhesion to be non-specific, the most likely situation is that a portion of the pilus was attached (white subunits) and not solely the adhesion pilin (red subunit). Only a part of the pilus (black subunits) was thus subjected to the applied force.</p

    Histograms of persistence length <i>L</i><sub><i>p</i></sub> (nm).

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    <p>The positions are provided by fitting WLC model to force spectroscopy data on the Pil strain and its pili-displaying derivatives with 1.5 μM BSA or 15 μM BSA for the Pil p<i>srtA</i> strain. Data were fitted using the Gaussian function (solid line) and Gaussian multi-peak analysis (solid line and black star (*)).</p

    Transmission electron micrograph of Pil strain.

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    <p>(A) Pil strain bacterium (scale bar is 500 nm). A close-up of a pilus (black square) is shown in (B) with higher contrast and (C) image analysis of the contour.</p
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