Atom Trap Trace Analysis of Rare Noble Gas Isotopes

<p>This presentation was used for the Low-Radioactivity Underground Argon Workshop held at Pacific Northwest National Laboratory in Richland, Washington on March 19 - 20, 2018.</p

Generation of a Doubly Bridging CO₂ Ligand and Deoxygenation of CO₂ by an (NHC)Ni(0) Complex

The treatment of [(IPr)Ni(μ-Cl)]2 (IPr = 1,3-bis(2,6-diisopropylphenyl)imidazol-2-ylidene) with NaOt-Bu and then pinB-Bpin (bis(pinacolato)diboron) gives rise to a mono-NHC−Ni(0) complex, which dimerizes upon crystallization. The same product is prepared by the treatment of [(IPr)Ni(μ-Cl)]2 with Li(BEt)3H, or with NaOt-Bu and then HSi(OEt)3. The treatment of [(IPr)Ni]2 or its monomer with CO2 reduces CO2 to CO generating [(IPr)Ni]2(μ-CO)(μ-η2,η2-CO2). This compound represents an unprecedented symmetric double-bridging mode of CO2

Generation of a Doubly Bridging CO₂ Ligand and Deoxygenation of CO₂ by an (NHC)Ni(0) Complex

The treatment of [(IPr)Ni(μ-Cl)]2 (IPr = 1,3-bis(2,6-diisopropylphenyl)imidazol-2-ylidene) with NaOt-Bu and then pinB-Bpin (bis(pinacolato)diboron) gives rise to a mono-NHC−Ni(0) complex, which dimerizes upon crystallization. The same product is prepared by the treatment of [(IPr)Ni(μ-Cl)]2 with Li(BEt)3H, or with NaOt-Bu and then HSi(OEt)3. The treatment of [(IPr)Ni]2 or its monomer with CO2 reduces CO2 to CO generating [(IPr)Ni]2(μ-CO)(μ-η2,η2-CO2). This compound represents an unprecedented symmetric double-bridging mode of CO2

Generation of a Doubly Bridging CO₂ Ligand and Deoxygenation of CO₂ by an (NHC)Ni(0) Complex

The treatment of [(IPr)Ni(μ-Cl)]2 (IPr = 1,3-bis(2,6-diisopropylphenyl)imidazol-2-ylidene) with NaOt-Bu and then pinB-Bpin (bis(pinacolato)diboron) gives rise to a mono-NHC−Ni(0) complex, which dimerizes upon crystallization. The same product is prepared by the treatment of [(IPr)Ni(μ-Cl)]2 with Li(BEt)3H, or with NaOt-Bu and then HSi(OEt)3. The treatment of [(IPr)Ni]2 or its monomer with CO2 reduces CO2 to CO generating [(IPr)Ni]2(μ-CO)(μ-η2,η2-CO2). This compound represents an unprecedented symmetric double-bridging mode of CO2

Generation of a Doubly Bridging CO₂ Ligand and Deoxygenation of CO₂ by an (NHC)Ni(0) Complex

The treatment of [(IPr)Ni(μ-Cl)]2 (IPr = 1,3-bis(2,6-diisopropylphenyl)imidazol-2-ylidene) with NaOt-Bu and then pinB-Bpin (bis(pinacolato)diboron) gives rise to a mono-NHC−Ni(0) complex, which dimerizes upon crystallization. The same product is prepared by the treatment of [(IPr)Ni(μ-Cl)]2 with Li(BEt)3H, or with NaOt-Bu and then HSi(OEt)3. The treatment of [(IPr)Ni]2 or its monomer with CO2 reduces CO2 to CO generating [(IPr)Ni]2(μ-CO)(μ-η2,η2-CO2). This compound represents an unprecedented symmetric double-bridging mode of CO2

Molecular weight (MW) and amino acid sequences of the cyclic (c) hexapeptides (c-WFW and c-WWW) and the magainin derivative (MK5E).

<p>alanine (A), arginine (R), glutamic acid (E), glycine (G), histidine (H), isoleucine (I), lysine (K), phenylalanine (F), serine (S), threonine (T), tryptophan (W), and valine (V). The linear peptide, MK5E is N-terminally acetylated (Ac) and C-terminally amidated (NH₂).</p

Motion parameters of boar spermatozoa after 5 min of incubation with increasing concentrations (5 µM, 10 µM, 20 µM, and 40 µM) of c-WFW, c-WWW, and MK5E (n = 12).

<p>Before the application of peptides, semen was preserved for 24°C in BTS containing 250 µg/mL gentamicin (standard). * Significant difference compared to standard (see text for the exact <i>P</i>-values).</p

Rotational correlation times (τ_c) for spin-labeled (SL) sphingomyelin (SL-SM), phosphatidylcholine (SL-PC), and phosphatidylserine (SL-PS) incorporated in the cell membrane of fresh boar sperm, recorded in BTS and in the presence of gentamicin, c-WFW, c-WWW, and MK5E at 16°C, 22°C, and 38°C, respectively.

<p><i>N</i>-(4-doxylpentanoyl)-<i>trans</i>-sphingenyl-1-phosphocholine (SL-SM).</p><p>1-Palmitoyl-2-(4-doxylpentanoyl)-phoshatidylcholine (SL-PC).</p><p>1-Palmitoyl-2-(4-doxylpentanoyl)-phosphatidylserine (SL-PS).</p><p>not determined (n. d.).</p>a<p>Values are means calculated from spectra of four independent sperm samples (SD<0.05).</p

Fecundity results with semen diluted in BTS +250 µg/mL gentamicin (standard) and 16 µg/mL gentamicin +4 µM c-WFW (peptide).

a<p>Two inseminations per sow were performed with 2×10⁹ sperm per dose.</p>b<p>Differences are not significant (<i>P</i>>0.05).</p

Motion parameters and percentage of damaged boar spermatozoa during BTS preservation at 16°C in the presence of 250 µg/mL gentamicin (standard), 4 µM and 8 µM c-WFW, 2 µM and 4 µM c-WWW, and 1 µM and 2 µM MK5E (n = 9).

<p>The percentage, the velocity (VAP), and the linearity (VSL/VCL) of progressive motile spermatozoa after 12 h and 96 h storage are shown (A, C, E). The percentage and the velocity (VAP) of progressive motile spermatozoa after 48 h storage are shown in a thermoresistance test (TRT, incubation at 38°C) for 30 and 300 min (B, D). The percentage of damaged spermatozoa was determined after 12 h, 48 h, and 96 h storage by staining with propidium iodide and/or markers for the acrosomal membrane and contents (F). Significant difference (see text for the exact <i>P</i>-values) compared to standard after 12 h (▪), 48 h (#), 96 h (▴) storage, and after TRT 30 min (○) and TRT 300 min (•) incubation at 38°C.</p