Supplementary Figures, Legends, and Code from Mitosis-Mediated Intravasation in a Tissue-Engineered Tumor–Microvessel Platform

Legends and still images for Supplemental Videos 1-13. Figure S1 provides a schematic illustration and images of device fabrication and setup. Figure S2 provides still images that summarize all intravasation events. Figure S3 provides still images of additional representative non-intravasation events following tumor cell division at the ECM-vessel interface. Figure S4 summarizes circularity and width measurements for all intravasation and non-intravasation events displayed in Figures S2 and S3. Figure S5 reveals anchored processes left behind after tumor cell intravasation. Figure S6 depicts multi-day tumor cell interactions at the ECM-vessel interface. Figure S7 shows a lack of tumor stem cell markers expressed by MDA-MB-231. Custom ImageJ code provided for quantifying maximum width of traced tumor cells.</p

Anisotropic Island Growth: A New Approach to Thin Film Electrocrystallization

The electrochemical deposition of metals onto foreign substrates usually occurs through Volmer−Weber island growth, and hence the structure and properties of thin films are critically dependent on the mechanism of nucleation and growth. For example, high nucleus densities are essential for achieving island coalescence at small thickness. Here we demonstrate a new approach to controlling thin film microstructure through the control of island geometry. By promoting anisotropic island growth, film coalescence can be achieved at smaller thickness and with lower island densities

Supplementary Video S5 from Mitosis-Mediated Intravasation in a Tissue-Engineered Tumor–Microvessel Platform

Mitosis-mediated intravasation.</p

Supplementary Video S10 from Mitosis-Mediated Intravasation in a Tissue-Engineered Tumor–Microvessel Platform

Exposed tumor cell detaching into vessel flow following mitosis.</p

Supplementary Video S12 from Mitosis-Mediated Intravasation in a Tissue-Engineered Tumor–Microvessel Platform

Tumor cell unable to perform transendothelial migration sheds portions or blebs into vessel flow.</p

Supplementary Video S9 from Mitosis-Mediated Intravasation in a Tissue-Engineered Tumor–Microvessel Platform

Transendothelial migration of an MDA-MB-231 breast cancer cell (BCC) following cell division without detaching into vessel flow.</p

Supplementary Video S7 from Mitosis-Mediated Intravasation in a Tissue-Engineered Tumor–Microvessel Platform

Tumor cell mitosis at the ECM-vessel interface without intravasation.</p

Supplementary Video S11 from Mitosis-Mediated Intravasation in a Tissue-Engineered Tumor–Microvessel Platform

Multiple tumor cells detaching into vessel flow following mitosis.</p

Supplementary Video S2 from Mitosis-Mediated Intravasation in a Tissue-Engineered Tumor–Microvessel Platform

Extended protrusions/invadopodia from an MDA-MB-231 breast cancer cell (BCC) are stabilized at the ECM-vessel interface.</p

Supplementary Video S4 from Mitosis-Mediated Intravasation in a Tissue-Engineered Tumor–Microvessel Platform

Mitosis-mediated intravasation.</p