Additional file 2: Figure S1B. of 3D culture of Her2+ breast cancer cells promotes AKT to MAPK switching and a loss of therapeutic response

Process of recovery of cells from 3D culture for experimental analysis. Cells were recovered from the 3D cultures for counting, immunocytochemical analysis or Western blotting as shown using a modification of a previously reported method (Arnold 2001). (DOC 52 kb

Additional file 3: Figure S2. of 3D culture of Her2+ breast cancer cells promotes AKT to MAPK switching and a loss of therapeutic response

Effects of AKT and MAPK inhibition on apoptosis in BT474 and MDAMB361 cells. Cell lines grown in 2D or 3D culture were exposed to AKT inhibitor (MK-2206) or MEK inhibitor (U0126) prior to cell lysis and Western blotting using an antibody that recognizes full length (116kDa) and cleaved (85kDa) forms of PARP, the latter form apparent upon apoptosis. Neither AKT or MAPK inhibition resulted in a significant loss of full length PARP or a corresponding gain in cleaved PARP for either cell line. In contrast, cleaved PARP was seen to increase in response to the apoptosis inducer, camptothecin, included as a positive control. (DOC 59 kb

Additional file 1: of The use of randomisation-based efficacy estimators in non-inferiority trials

Data assumptions made for the ZICE trial. Descriptions of how the adherence and outcome data were derived for the ZICE study. Sensitivity analysis exploring the impact of missing data on the interpretation of the SMM analysis in the ZICE trial. (DOCX 20 kb