The literature search process.

<p>Flow diagram depicts the screening process of retrieved articles, including the reason for and number of exclusions. CFH = complement factor H; PCV = polypoidal choroidal vasculopathy.</p

Cumulative meta-analysis of the association between I62V polymorphism and PCV.

<p>Every circle represents the pooled OR when studies accumulated over time, and the horizontal line represents the 95% confidence interval of pooled OR. A: OR₁(GG+GA vs AA); B: OR₂ (GA vs AA); C: OR₃ (GG vs AA); D: OR₄ (G vs A).</p

Results of Leave-One-Out Sensitivity Analysis.

<p>The horizontal axis shows the omitted study. The horizontal axis represents the odds ratio. Every circle indicates the pooled OR when the left study is omitted in this meta-analysis. The two ends of every broken line represent the respective 95% confidence interval. A: OR₁(GG+GA vs AA); B: OR₂ (GA vs AA); C: OR₃ (GG vs AA); D: OR₄ (G vs A).</p

Systematic Review and Meta-Analysis of the Association between Complement Factor H I62V Polymorphism and Risk of Polypoidal Choroidal Vasculopathy in Asian Populations

<div><p>Purpose</p><p>To investigate whether the polymorphism rs800292 (184G>A, I62V) in the complement factor H gene is associated with polypoidal choroidal vasculopathy (PCV) and the genetic difference between PCV and neovascular age-related macular degeneration (nAMD), in Asian populations.</p><p>Methods</p><p>A comprehensive literature search was performed in PubMed, Medline, Web of Science, and reference lists. A system review and meta-analysis of the association between I62V and PCV and/or nAMD were performed from 8 studies involving 5,062 subjects. The following data from individual studies were extracted and analyzed: 1) comparison of I62V polymorphisms between PCV and controls; 2) comparison of I62V polymorphisms between PCV and nAMD. Summary odds ratios (ORs) and 95% confidence intervals (CIs) were estimated using fixed-effects models. The Q-statistic test was used to assess heterogeneity, and Egger’s test was used to evaluate publication bias. Sensitivity analysis and cumulative meta-analysis were also performed.</p><p>Results</p><p>The I62V polymorphism showed a significant summary OR₁ for genotype GA+GG versus homozygous genotype AA was 3.18 (95% CI, 2.51–4.04, <i>P</i><<i>0.00001</i>), the OR₂ of heterozygous genotype GA versus AA was 2.29 (95% CI: 1.79–2.94, <i>P</i><<i>0.00001</i>), the OR₃ of homozygous genotype GG versus AA was 4.42 (95% CI: 3.45–5.67, <i>P</i><<i>0.00001</i>), and the OR₄ of allele G versus A was 2.04 (95% CI: 1.85–2.26, <i>P</i><<i>0.00001</i>). Sensitivity analysis indicated the robustness of our findings, and evidence of publication bias was not observed in our meta-analysis. Cumulative meta-analysis revealed that the summary ORs were stable. There was no significant difference in every genetic model between PCV and nAMD (n = 5, OR₁ = 0.92, OR₂ = 0.96, OR₃ = 0.90, OR₄ = 0.94).</p><p>Conclusions</p><p>Our analysis provides evidence that the I62V polymorphism is associated with an increased risk of PCV. The variant of I62V could be a promising genetic biomarker of PCV in Asian populations.</p></div

Forest plots of meta-analysis of the association between I62V polymorphism and PCV.

<p>Odds ratios (black <i>squares)</i> and 95% confidence intervals (bars) are given for each study. Also shown are the <i>diamonds</i> of the summary ORs based on the Mantel–Haenszel fixed-effects model (M-H Overall). CI = confidence interval; OR = odds ratio. A: OR₁(GG+GA vs AA); B: OR₂ (GA vs AA); C: OR₃ (GG vs AA); D: OR₄ (G vs A).</p

Allele and Genotype Distribution of the I62V Polymorphism.

<p>HWE = HardyeWeinberg equilibrium; NA = not available; nAMD = neovascular age-related macular degeneration; PCV = polypoidal choroidal vasculopathy.</p

Main Characteristics of the Studies Included in the Meta-Analysis.

<p>NA = not available; nAMD = neovascular age-related macular degeneration; PCV = polypoidal choroidal vasculopathy.</p><p>*The number of cases or controls was changed in the next meta-analysis, but the reason was not given.</p

Meta-analysis compared the allelic frequencies of I62V between PCV and nAMD.

<p>CI = confidence interval; M-H = Mantel–Haenszel model; nAMD = neovascular age-related macular degeneration; OR = odds ratio; PCV = polypoidal choroidal vasculopathy.</p