Acetylcholinesterase inhibitory active metabolites from the endophytic fungus <i>Colletotrichum</i> sp. YMF432

<p>An endophytic fungus, <i>Chaetomium</i> sp. YMF432, was isolated from <i>Huperzia serrata</i> (Thunb. ex Murray) Trev. and subjected to phytochemical investigation based on its special environment. From the extracts of fermentation solid of strain YMF 432, eight compounds including 1-<i>O</i>-methylemodin (<b>1</b>), 5-methoxy-2-methyl-3-tricosyl-1,4-benzoquinone (<b>2</b>), 4,8-dihydroxy-1-tetralone (<b>3</b>), (3β,5α,6α, 22<i>E</i>)-3-hydroxy-5,6-epoxy-7-one-8(14),22-dien-ergosta (<b>4</b>), ergosta-4,6,8(14),22-tetraen-3-one (<b>5</b>), β-sitostenone (<b>6</b>), β-sitosterol (<b>7</b>) and (22<i>E</i>,24<i>R</i>)-ergosta-5,7,22 -trien-3β-ol (<b>8</b>) were obtained. Their structures were elucidated on the basis of their spectroscopic data. These compounds were evaluated for acetylcholinesterase inhibitory activities <i>in vitro</i>. Compounds <b>1</b>, <b>2</b>, and <b>4</b> showed moderate acetylcholinesterase inhibitory activities (IC₅₀ from 37.7 ± 1.5 to 370.0 ± 2.9 μM).</p

Two new aromadendrane sesquiterpenes from <i>Verticillium psalliotae</i>

<p>The chemical constituents of the fungus <i>Verticillium psalliotae</i> were studied. Two new aromadendrane sesquiterpenes inonotin M (<b>1</b>) and inonotin N (<b>2</b>) were isolated from the EtOAc extract of the fungal culture broth. The structures of compounds were elucidated mainly by HRESIMS experiments, and 1D, 2D-NMR spectroscopy analysis.</p

Naphthomycins L–N, Ansamycin Antibiotics from <i>Streptomyces</i> sp. CS

Previous analyses of the naphthomycin biosynthetic gene cluster and a comparison with known naphthomycin-type products from <i>Streptomyces</i> sp. CS have suggested that new products can be found from this strain. In this study, screening by LC-MS of <i>Streptomyces</i> sp. CS products formed under different culture conditions revealed several unknown peaks in the product spectra of extracts derived from oatmeal medium cultures. Three new naphthomycins, naphthomycins L (<b>1</b>), M (<b>2</b>), and N (<b>3</b>), and the known naphthomycins A (<b>4</b>), E (<b>5</b>), and D (<b>6</b>) were obtained. The structures were elucidated using spectroscopic data from 1D and 2D NMR and HRESIMS experiments