Additional file 2: of A randomised, double-blind, placebo-controlled phase 1 study of the safety, tolerability and pharmacodynamics of volixibat in overweight and obese but otherwise healthy adults: implications for treatment of non-alcoholic steatohepatitis

Table S1. Schedule of study assessments. (PDF 69 kb

Additional file 1: of A randomised, double-blind, placebo-controlled phase 1 study of the safety, tolerability and pharmacodynamics of volixibat in overweight and obese but otherwise healthy adults: implications for treatment of non-alcoholic steatohepatitis

Inclusion and exclusion criteria. (PDF 65 kb

Development of a new bicistronic retroviral vector with strong IRES activity-2

<p><b>Copyright information:</b></p><p>Taken from "Development of a new bicistronic retroviral vector with strong IRES activity"</p><p>BMC Biotechnology 2006;6():4-4.</p><p>Published online 12 Jan 2006</p><p>PMCID:PMC1373653.</p><p>Copyright © 2006 Martin et al; licensee BioMed Central Ltd.</p>as been cloned in the polylinker, while pPRIG-Hd-HA-Red is the same exact construct except that the IRES initiation codon is modified into a HindIII site (see Table 1). The psi letter (Ψ) is the MLV encapsidation sequence, the CMV promoter drives the expression of the bicistronic RNA, the hatched boxes flanking DsRed correspond to the polylinker, encompassing the T7 and SP6 sequences and the HA coding sequence, the IRES corresponds to the EMCV-derived IRES fragment, eGFP is the coding sequence for green fluorescent protein used to track the first cistron expression, and the LTR corresponds to the MLV long terminal repeat. The hatched line symbolizes Puc-derived plasmid backbone

Development of a new bicistronic retroviral vector with strong IRES activity-1

<p><b>Copyright information:</b></p><p>Taken from "Development of a new bicistronic retroviral vector with strong IRES activity"</p><p>BMC Biotechnology 2006;6():4-4.</p><p>Published online 12 Jan 2006</p><p>PMCID:PMC1373653.</p><p>Copyright © 2006 Martin et al; licensee BioMed Central Ltd.</p>dditional 1-nucleotide frame-shifted coding sequences for HA-tagging 5' of the polylinker sequence. Any insert can thus be cloned either in-frame downstream of this HA-tag, or directly into the pPRIG, without the HA-tag

Development of a new bicistronic retroviral vector with strong IRES activity-4

<p><b>Copyright information:</b></p><p>Taken from "Development of a new bicistronic retroviral vector with strong IRES activity"</p><p>BMC Biotechnology 2006;6():4-4.</p><p>Published online 12 Jan 2006</p><p>PMCID:PMC1373653.</p><p>Copyright © 2006 Martin et al; licensee BioMed Central Ltd.</p>al supernatants were collected after 2 days and applied to primary rat embryo fibroblasts. The REF cells were photographed two weeks post-transduction. Vis: phase contrast image. Exposure time was the same for the three GFP images, as well as for the three RFP images. Fluorescence intensities are thus a direct reflection of protein expression levels

Structure Evolution and Drying Dynamics in Sliding Cholesteric Cellulose Nanocrystals

The study of colloidal liquid crystals (LCs) reveals fundamental insights into the nature of ordered materials, giving rise to emergent properties with fascinating applications in soft matter nanotechnology. Here we investigate the shape instabilities, layer undulations, dynamic assembly, and collective behaviors in evaporating a cellulose nanocrystal-based cholesteric LC drop. During the drying process, the drop edges are pinned to the substrate with spontaneous convective flow occurring along the drop, which leads to nonequilibrium sliding of the individual cholesteric fragment with active ordering as well as hydrodynamic fluctuations and flow transitions in the bulk cholesteric phase

Development of a new bicistronic retroviral vector with strong IRES activity-5

<p><b>Copyright information:</b></p><p>Taken from "Development of a new bicistronic retroviral vector with strong IRES activity"</p><p>BMC Biotechnology 2006;6():4-4.</p><p>Published online 12 Jan 2006</p><p>PMCID:PMC1373653.</p><p>Copyright © 2006 Martin et al; licensee BioMed Central Ltd.</p> from the indicated vectors were loaded in duplicate, ran on SDS-PAGE, and analyzed with an anti GFP serum (left panel) and an anti-HA serum (right panel)

Development of a new bicistronic retroviral vector with strong IRES activity-3

<p><b>Copyright information:</b></p><p>Taken from "Development of a new bicistronic retroviral vector with strong IRES activity"</p><p>BMC Biotechnology 2006;6():4-4.</p><p>Published online 12 Jan 2006</p><p>PMCID:PMC1373653.</p><p>Copyright © 2006 Martin et al; licensee BioMed Central Ltd.</p>phed 48 h later. Vis: phase contrast image. Exposure time is the same for the two GFP images, and for the two RFP images. Fluorescence signals are thus a direct reflection of protein expression levels

Structure Evolution and Drying Dynamics in Sliding Cholesteric Cellulose Nanocrystals

The study of colloidal liquid crystals (LCs) reveals fundamental insights into the nature of ordered materials, giving rise to emergent properties with fascinating applications in soft matter nanotechnology. Here we investigate the shape instabilities, layer undulations, dynamic assembly, and collective behaviors in evaporating a cellulose nanocrystal-based cholesteric LC drop. During the drying process, the drop edges are pinned to the substrate with spontaneous convective flow occurring along the drop, which leads to nonequilibrium sliding of the individual cholesteric fragment with active ordering as well as hydrodynamic fluctuations and flow transitions in the bulk cholesteric phase

Structure Evolution and Drying Dynamics in Sliding Cholesteric Cellulose Nanocrystals

The study of colloidal liquid crystals (LCs) reveals fundamental insights into the nature of ordered materials, giving rise to emergent properties with fascinating applications in soft matter nanotechnology. Here we investigate the shape instabilities, layer undulations, dynamic assembly, and collective behaviors in evaporating a cellulose nanocrystal-based cholesteric LC drop. During the drying process, the drop edges are pinned to the substrate with spontaneous convective flow occurring along the drop, which leads to nonequilibrium sliding of the individual cholesteric fragment with active ordering as well as hydrodynamic fluctuations and flow transitions in the bulk cholesteric phase