Additional file 6: Figure S5. of LIX1 regulates YAP1 activity and controls the proliferation and differentiation of stomach mesenchymal progenitors

Sustained LIX1 expression does not affect apoptosis. Transverse sections of E6 control and LIX1-expressing stomachs analysed by immunohistochemistry with anti-cleaved CASPASE-3 antibodies. Black arrows indicate the area imaged at high power in the cleaved-CASPASE-3 panels. Black arrowheads point to cleaved CASPASE-3-positive apoptotic cells. (JPG 402 kb

Additional file 12. of LIX1 regulates YAP1 activity and controls the proliferation and differentiation of stomach mesenchymal progenitors

Supporting data. (XLSX 46 kb

Additional file 4: Table S1. of LIX1 regulates YAP1 activity and controls the proliferation and differentiation of stomach mesenchymal progenitors

Phenotype quantification. Quantification of embryos harbouring an abnormal stomach muscle phenotype, as demonstrated by in situ hybridization or immunostaining, following injection of RCAS(A)-ShLIX1 (LIX1 loss-of-function) or RCAS(B)-LIX1 (LIX1 gain-of-function). (PDF 40 kb

Additional file 10: Figure S9. of LIX1 regulates YAP1 activity and controls the proliferation and differentiation of stomach mesenchymal progenitors

Infection with RCAS-GFP retroviruses does not affect chick stomach development and differentiation. (A) Whole-mount in situ hybridization of E6 uninfected (control) and GFP-expressing gastrointestinal tracts using SM22, BAPX1 and SOX10 riboprobes. Scale bars, 1 mm. (B) Transverse sections of E7 control and GFP-expressing stomachs analysed either by in situ hybridization using the LIX1 riboprobe or by immunofluorescence with anti-αSMA and anti-CALPONIN antibodies. Nuclei were visualized with Hoechst. (C) Serial transverse sections of E7 control and GFP-expressing stomachs analysed by immunofluorescence using anti-PH3 antibodies. Nuclei were visualized with Hoechst. Graph represents the quantification of PH3-positive cells. ns, Not significant. (JPG 1115 kb