Navigational style influences eye movement pattern during exploration and learning of an environmental map

During navigation people may adopt three different spatial styles (i.e., Landmark, Route, and Survey). Landmark style (LS) people are able to recall familiar landmarks but cannot combine them with directional information; Route style (RS) people connect landmarks to each other using egocentric information about direction; Survey style (SS) people use a map-like representation of the environment. SS individuals generally navigate better than LS and RS people. Fifty-one college students (20 LS; 17 RS, and 14 SS) took part in the experiment. The spatial cognitive style (SCS) was assessed by means of the SCS test; participants then had to learn a schematic map of a city, and after 5 min had to recall the path depicted on it. During the learning and delayed recall phases, eye-movements were recorded. Our intent was to investigate whether there is a peculiar way to explore an environmental map related to the individual's spatial style. Results support the presence of differences in the strategy used by the three spatial styles for learning the path and its delayed recall. Specifically, LS individuals produced a greater number of fixations of short duration, while the opposite eye movement pattern characterized SS individuals. Moreover, SS individuals showed a more spread and comprehensive explorative pattern of the map, while LS individuals focused their exploration on the path and related targets. RS individuals showed a pattern of exploration at a level of proficiency between LS and SS individuals. We discuss the clinical and anatomical implications of our data

Cell Propagation of Cholera Toxin CTA ADP-Ribosylating Factor by Exosome Mediated Transfer

In this study, we report how the cholera toxin (CT) A subunit (CTA), the enzyme moiety responsible for signaling alteration in host cells, enters the exosomal pathway, secretes extracellularly, transmits itself to a cell population. The first evidence for long-term transmission of CT's toxic effect via extracellular vesicles was obtained in Chinese hamster ovary (CHO) cells. To follow the CT intracellular route towards exosome secretion, we used a novel strategy for generating metabolically-labeled fluorescent exosomes that can be counted by flow cytometry assay (FACS) and characterized. Our results clearly show the association of CT with exosomes, together with the heat shock protein 90 (HSP90) and Protein Disulfide Isomerase (PDI) molecules, proteins required for translocation of CTA across the ER membrane into the cytoplasm. Confocal microscopy showed direct internalization of CT containing fluorescent exo into CHO cells coupled with morphological changes in the recipient cells that are characteristic of CT action. Moreover, Me665 cells treated with CT-containing exosomes showed an increase in Adenosine 3',5'-Cyclic Monophosphate (cAMP) level, reaching levels comparable to those seen in cells exposed directly to CT. Our results prompt the idea that CT can exploit an exosome-mediated cell communication pathway to extend its pathophysiological action beyond an initial host cell, into a multitude of cells. This finding could have implications for cholera disease pathogenesis and epidemiology

High/Other Looks. Different ‘Points of View’ in 20th Century Art

In 1960 Jorge Luis Borges (1899-1986) published El hacedor, a collection of short stories and poems through which he narrates personal events and impressions, something unusual for the Argentine writer. [read more

Goteira oclusal na otimização da performance desportiva: estado da arte

A maior parte dos atletas procura novos métodos para melhorar a sua performance. Têm surgido vários estudos sobre o uso de uma goteira oclusal para melhorar o desempenho dos atletas mesmo sem disfunções temporomandibulares. O objetivo desta revisão narrativa foi tentar de esclarecer, através da análise da literatura existente, se a aplicação de um dispositivo intraoral pode de facto melhorar a performance do atleta. A metodologia consistiu numa pesquisa efetuada nas bases de dados Pubmed, Medline, Scielo e Google Académico. A aplicação de uma goteira oclusal poderá ter impacto no rendimento físico do atleta, no entanto os resultados não são concordantes nas várias tipologias de performance analisadas e por isso serão necessários mais estudos com maior duração, amostras maiores, métodos de pesquisa mais padronizados de forma a esclarecer o papel da goteira no âmbito desportivo.Most athletes are constantly looking for new methods to improve their performance. For many years it has been discussed the supposed utility of using an occlusal splint to improve the performance of athletes even without temporomandibular disorders. The purpose of this narrative review was to try to clarify, through the analysis of the existing Literature, if the application of an intraoral device can improve the performance of the athlete. The methodology consisted of a search conducted on databases Pubmed, Medline, Scielo e Google Scholar. It seems that the craniofacial muscles have some influence on the remaining muscles of the body. The application of an occlusal splint yielded discordant results in the various performance typologies analyzed, so we can conclude that more studies with duration and larger sample and a more standardized research method are needed

On the Fundamental Periods of Vibration of Flat-Bottom Ground-Supported Circular Silos containing Gran-like Material

Despite the significant amount of research effort devoted to understanding the structural behavior of grain-silos, each year a large number of silos still fails due to bad design, poor construction, with a frequency much larger than other civil structures. In particular, silos frequently fails during large earthquakes, as occurred during the 1999 Chi-Chi, Taiwan earthquake when almost all the silos located in Taichung Port, 70 km far from the epicenter, collapsed. The EQE report stated that "the seismic design of practice that is used for the design and construction of such facilities clearly requires a major revision". The fact indicates that actual design procedures have limits and therefore significant advancements in the knowledge of the structural behavior of silo structures are still necessary. The present work presents an analytical formulation for the assessment of the natural periods of grain silos. The predictions of the novel formulation are compared with experimental findings and numerical simulations

Numerical modeling of active thermo-plasmonics experiments

In this paper, we present a simple and robust numerical method able to predict, with high accuracy, the photo-thermal effects occurring for a gold nanoparticles arrangement under externally applied strain. The physical system is numerically implemented in the COMSOL Multiphysics simulation platform. The gold nanoparticles distributions are excited by linearly polarized light. By considering the system at rest and under the action of a mechanical stress, we analyze the extinction cross section, and we observe the production of heat at the nanoscale. The purpose of this work is to describe how sensitive the local temperature of the gold nanoparticles arrangement is to the formation of localized photo-thermal hot spots

Mass Mortality Event of Mediterranean Mussels (Mytilus galloprovincialis) in the Middle Adriatic: Potential Implications of the Climate Crisis for Marine Ecosystems

The effects of the climate crisis are affecting ecosystems at different scales and magnitudes. This paper focuses on a massive Mediterranean mussel die-off observed along the middle Italian Adriatic coast in the summer of 2022. We considered the possible environmental causes of this phenomenon and carried out a climatic analysis of the last decade. We performed field surveys in different locations along a 16 km coastal stretch from Martinsicuro (TE) in the south, to Grottammare (AP) in the north. The study area includes two marine Sites of Community Importance under the European Natura 2000 network. The die-off of the mussels was observed in practically all the natural mussel beds colonizing the study area. As sessile filter-feeding organisms inhabiting the intertidal zone, mussels are highly exposed to variations in environmental conditions such as temperature and nutrient load. We discuss the possible causes of this die-off, proposing that high temperature and the scarce availability of food acted simultaneously as stress factors, generating local unsustainable living conditions for this species

Neuromorphic vibrotactile stimulation of fingertips for encoding object stiffness in telepresence sensory substitution and augmentation applications

We present a tactile telepresence system for real-time transmission of information about object stiffness to the human fingertips. Experimental tests were performed across two laboratories (Italy and Ireland). In the Italian laboratory, a mechatronic sensing platform indented different rubber samples. Information about rubber stiffness was converted into on-off events using a neuronal spiking model and sent to a vibrotactile glove in the Irish laboratory. Participants discriminated the variation of the stiffness of stimuli according to a two-alternative forced choice protocol. Stiffness discrimination was based on the variation of the temporal pattern of spikes generated during the indentation of the rubber samples. The results suggest that vibrotactile stimulation can effectively simulate surface stiffness when using neuronal spiking models to trigger vibrations in the haptic interface. Specifically, fractional variations of stiffness down to 0.67 were significantly discriminated with the developed neuromorphic haptic interface. This is a performance comparable, though slightly worse, to the threshold obtained in a benchmark experiment evaluating the same set of stimuli naturally with the own hand. Our paper presents a bioinspired method for delivering sensory feedback about object properties to human skin based on contingency-mimetic neuronal models, and can be useful for the design of high performance haptic devices