Characterization of <i>MORE AXILLARY GROWTH</i> Genes in <i>Populus</i>

<div><p>Background</p><p>Strigolactones are a new class of plant hormones that play a key role in regulating shoot branching. Studies of branching mutants in Arabidopsis, pea, rice and petunia have identified several key genes involved in strigolactone biosynthesis or signaling pathway. In the model plant Arabidopsis, <i>MORE AXILLARY GROWTH1</i> (<i>MAX1</i>), <i>MAX2</i>, <i>MAX3</i> and <i>MAX4</i> are four founding members of strigolactone pathway genes. However, little is known about the strigolactone pathway genes in the woody perennial plants.</p><p>Methodology/Principal Finding</p><p>Here we report the identification of MAX homologues in the woody model plant <i>Populus trichocarpa</i>. We identified the sequence homologues for each MAX protein in <i>P. trichocarpa</i>. Gene expression analysis revealed that <i>Populus MAX</i> paralogous genes are differentially expressed across various tissues and organs. Furthermore, we showed that <i>Populus MAX</i> genes could complement or partially complement the shoot branching phenotypes of the corresponding Arabidopsis <i>max</i> mutants.</p><p>Conclusion/Significance</p><p>This study provides genetic evidence that strigolactone pathway genes are likely conserved in the woody perennial plants and lays a foundation for further characterization of strigolactone pathway and its functions in the woody perennial plants.</p></div

居民組織

2003-2004 > Academic research: refereed > Chapter in an edited book (author

MOESM1 of Simultaneous knockdown of six non-family genes using a single synthetic RNAi fragment in Arabidopsis thaliana

Additional file 1: Figure S1. Alignment of cDNAs of AtHY2, AtTRY, AtLNG1, AtNPQ1, AtSEX1, AtMAX3 and AtGUN4

Expression of <i>Populus MAX</i> homologous genes across various tissues and organs.

<p>(<b>A</b>) Illustration of tissues and organs used for expression analysis. (<b>B</b>) Quantitative RT-PCR data. Shown are means ± S.E. of three biological replicates.</p

Genetic complementation of <i>Arabidopsis max4</i> mutants with <i>Populus MAX4</i> genes.

<p>(<b>A</b>) RT-PCR analysis of <i>35S:PtrMAX4a</i> transgenic lines. (<b>B</b>) RT-PCR analysis of <i>35S:PtrMAX4b</i> transgenic lines. (<b>C</b>) Number of primary rosette-leaf branches. Shown are average numbers of primary rosette-leaf branches from at least 10 individual plants ± S.E. *, significant difference from <i>max4-1</i>, p<0.05.</p

Genetic complementation of <i>Arabidopsis max2</i> mutants with <i>Populus MAX2</i> genes.

<p>(<b>A</b>) RT-PCR analysis of <i>35S:PtrMAX2a</i> transgenic lines. (<b>B</b>) RT-PCR analysis of <i>35S:PtrMAX2b</i> transgenic lines. (<b>C</b>) Number of primary rosette-leaf branches. Shown are average numbers of primary rosette-leaf branches from at least 10 individual plants ± S.E. *, significant difference from <i>max2-4</i>, p<0.05.</p

Genetic complementation of <i>Arabidopsis max1</i> mutants with <i>Populus MAX1</i> genes.

<p>(<b>A</b>) RT-PCR analysis of <i>35S:PtrMAX1a</i> transgenic lines. (<b>B</b>) RT-PCR analysis of <i>35S:PtrMAX1b</i> transgenic lines. (<b>C</b>) Number of primary rosette-leaf branches. Shown are average numbers of primary rosette-leaf branches from at least 10 individual plants ± S.E. *, significant difference from <i>max1-4</i>, p<0.05.</p

Strigolactone-Regulated Proteins Revealed by iTRAQ-Based Quantitative Proteomics in <i>Arabidopsis</i>

Strigolactones (SLs) are a new class of plant hormones. In addition to acting as a key inhibitor of shoot branching, SLs stimulate seed germination of root parasitic plants and promote hyphal branching and root colonization of symbiotic arbuscular mycorrhizal fungi. They also regulate many other aspects of plant growth and development. At the transcription level, SL-regulated genes have been reported. However, nothing is known about the proteome regulated by this new class of plant hormones. A quantitative proteomics approach using an isobaric chemical labeling reagent, iTRAQ, to identify the proteome regulated by SLs in <i>Arabidopsis</i> seedlings is presented. It was found that SLs regulate the expression of about three dozen proteins that have not been previously assigned to SL pathways. These findings provide a new tool to investigate the molecular mechanism of action of SLs