UJI AKTIVITAS ANTIMIKROBA FRAKSI DAUN JERUK LEMON (Citrus limon (L.) Osbeck) TERHADAP Stapylococcus aureus DAN Escherichia coli

Infection is a condition where the entry of microorganisms into the body's tissues, multiplies and causes disease in the form of disruption of body functions. Lemon leaves (Citrus limon (L.) Osbeck) are plants that have the potential to have antimicrobial power in the presence of bioactive compounds. The purpose of this study was to determine the antimicrobial activity of lemon fraction against Staphylococcus aureus and Escherichia coli. Ethyl acetate fraction is more effective in inhibiting bacteria, compared to ethanol and n-hexane extract based on preliminary tests conducted. Disk diffusion antimicrobial activity test (Kirby-Bauer) was used at a concentration of 5% the ethyl acetate fraction effectively inhibited Staphylococcus aureus bacteria with a diameter of inhibition zone was 12.3 mm while the Escherichia coli bacteria was 8.7 mm at a concentration of 25%. The results of data analysis using One-Way ANOVA (analysis of variance) showed a significant difference based on variations in concentration in inhibiting Staphylococcus aureus and Escherichia coli

OPTIMALISASI SUMBER DAYA LOKAL DALAM PENANGANAN HIPERTENSI DENGAN PUHAT LASIBUGA (PUDING SEHAT LABU SIAM BUAH NAGA)

Hipertensi adalah kondisi tekanan darah tinggi dengan sistolik (TDS) ? 140 mmHg dan tekanan darah diastolik TDS) ? 90 mmHg. Hipertensi di Indonesia merupakan ”silent killer”, banyak orang tak merasakan gejala. Penting periksa tekanan darah rutin. 1 dari 3 orang di Indonesia mengidap hipertensi, angka terus meningkat, termasuk wilayah Bekasi, tepatnya di RT 04/RW.01 Kel. Sepanjang Jaya, Bekasi Timur terdapat 70% masyarkat dengan hipertensi. Tujuan penelitian: unntuk mengkaji lebih dalam tentang pengoptimalkan sumber daya local dalam penanganan hipertensi dengan PUHAT LASIBUGA (Puding Sehat Labu Siam Buah Naga). Metode penelitian: penelitian menggunakan metode kuantitatif dengan desain One Group Pre Test Design. Dengan teknik Accidental Sampling terdiri dari 30 masyarakat setempat. Hasil penelitian: pengumpulan data dilakukan selama 4 hari, dengan hasil menunjukan, hasil pre test 2,80 dan post test 4,77 dengan sig:0,000 yang berarti lebih kecil dari 0,05, sehingga H0 ditolak dan H1 diterima yang artinya terajdi peningkatan optimalnya sumber daya lokal di masyarakat

Antiproliferative Activity of Xanthones Isolated from Artocarpus obtusus.

An investigation of the chemical constituents in Artocarpus obtusus species led to the isolation of three new xanthones, pyranocycloartobiloxanthone A (1), dihydroartoindonesianin C (2), and pyranocycloartobiloxanthone B (3). The compounds were subjected to antiproliferative assay against human promyelocytic leukemia (HL60), human chronic myeloid leukemia (K562), and human estrogen receptor (ER+) positive breast cancer (MCF7) cell lines. Pyranocycloartobiloxanthone A (1) consistently showed strong cytotoxic activity against the three cell lines compared to the other two with IC 50 values of 0.5, 2.0 and 5.0 μg/mL, respectively. Compound (1) was also observed to exert antiproliferative activity and apoptotic promoter towards HL60 and MCF7 cell lines at respective IC 50 values. The compound (1) was not toxic towards normal cell lines human nontumorigenic breast cell line (MCF10A) and human peripheral blood mononuclear cells (PBMCs) with IC 50 values of more than 30 μg/mL

Chemical constituents and biological activities of two rutaceous plants : Clausena excavata Burm. and Evodia malayana Ridl.

Phytochemical and biological activity studies on two Sumatran Rutaceae plants were carried out. Both Clausena excavata Burm. and Evodia malayana Ridl., were subjected to detail isolation work. The plants were extracted by using three organic solvents of different polarity, followed by isolation of the compounds by using several common chromatography techniques, such as gravity column chromatography, vacuum flash chromatography, radial chromatography and preparative thin layer chromatography. The structural elucidations of isolated compound were carried out by using various spectroscopic techniques including infrared (IR), mass spectroscopy (MS), nuclear magnetic resonance (NMR) and also by comparison with literature data. The phytochemical investigations have led to the isolation of several compounds of different classes, including coumarins, alkaloid, flavonoid, lignan, acridin derivative and phytosterols. The crude extracts and some of the isolated compounds were screened for antimicrobial, antioxidant and cytotoxic activities, using disc diffusion method, DPPH (1,1-diphenyl-2-picrylhydrazyl) and MTT (Microculture Tetrazolium Salt) assay, respectively. The antimicrobial activity was tested against nine selected bacteria named Bacillus cereus, Bacillus subtilis, Escherichia coli, Enterobachter aerogenes, Klebsiella pneumonia, Salmonella typhimurium, Staphylococcus aureus, Aspergillus niger and Candida albicans, respectively. The cell line used in cytotoxic assay was MCF-7, the human adenocarcinoma breast cancer cell line. The phytochemical study on Clausena excavata Burm. has led to the isolation of four coumarins, seselin (141), imperatorin (65), xanthyletin (37), xantotoxol (142), an alkaloid, murrayanin (143) and β-sitosterol (140). From the isolation work on Evodia malayana Burm. has yielded a lignan, sesamin (145), a flavonoid, pachypodol (146), an acridin derivative, 1,3-dimethoxy-10H-acridin-9-one (147) together with two phytosterols β-sitosterol (140) and stigmasterol (144). This acridin derivative was firstly reported to be isolated from natural origin and also new constituent to E. malayana Ridl. Among the isolated compounds, imperatorin (65) and xanthotoxol (37) exhibited the moderate activity toward Escherichia coli, Enterobachter aerogenes and Staphylococcus aureus. In cytotoxic study, seselin (141), xanthotoxol (142) and sesamin (145) displayed remarkable potential activity against MCF-7 cell line, while potential radical scavenging activity was shown by xanthotoxol (142). Xanthotoxol (142) showed the excellent activities in all assays which have been conducted in this study, and becomes a strong reason for further drug discovery study