Application of Different Temperatures in the Incubation Process of Jurung Fish Eggs (Tor tambra)

The habitat of the Jurung Fish (Tor tambra) is generally inflowing rivers, rocky bottom waters and relatively low water temperatures. This study aimed to determine the hatchability of the Jurung Fish (Tor tambra) eggs with different temperature treatments using a tray in the aquarium. The research method used is the laboratory experimental method and the experimental design is a non-factorial completely randomized design (CRD) consisting of 4 treatments and 4 replications: A (control), B (28°C), C (30°C) and D (32°C). The results showed that the application of different temperatures had a significant effect on the hatching of the Jurung Fish (Tor tambra) eggs. The results of Duncan's further test analysis showed that the 28°C temperature treatment was the best compared to other temperatures. This study recommends that further research is needed on larval abnormalities at different water temperatures

PEMANFAATAN PROBIOTIK RABAL DALAM PAKAN KOMERSIAL TERHADAP PERTUMBUHAN BENIH IKAN NILA SULTANA DENGAN SISTEM BUDIDAYA DALAM EMBER

Budidaya dalam ember merupakan wadah budidaya yang menggunakan ember. Ikan nila (Oreochromis niloticus) merupakan jenis ikan air tawar dengan kualitas adaptasi diri yang baik, sehingga menjadi komoditas unggulan untuk budidaya. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian probiotik rabal pada pakan komersil terhadap pertumbuhan (Oreochromis niloticus L) dengan sistem budidaya dalam ember. Penelitian ini dilaksanakan di Laboratorium Basah Program Studi Teknologi Produksi Benih dan Pakan Ikan Politeknik Indonesia Venezuela (POLIVEN) pada bulan agustus s/d september 2022. Metode yang digunakan adalah metode Rancangan Acak Lengkap (RAL) nonfaktorial yang terdiri dari 4 perlakuan dan 4 ulangan yaitu A (Kontrol), B (25 ml probiotik rabal), C (30 ml probiotik rabal), dan D (35 ml). Hasil penelitian menunjukkan penambahan probiotik rabal pada pakan komersil berpengaruh nyata terhadap laju pertumbuhan spesifik dengan nilai tertinggi terdapat pada perlakuan D (35 ml) dengan nilai rata-rata 0,036 gram/hari, Akan tetapi tidak berpengaruh nyata terhadap kelangsungan hidup, panjang mutlak, dan rasio konversi pakan.Pada kelangsungan hidup terbaik terdapat pada perlakuan D (35 m) yaitu mencapai 80%. Nilai Tertinggi panjang mutlak terdapat pada perlakuan D (35 ml) dengan nilai rata-rata mencapai 1,88 cm, sedangkan pada rasio konversi pakan nilai terbaik terdapat pada perlakuan D (35 ml) dengan nilai 0,62. Berdasarkan hasil uji lanjut Duncan perlakuan D (35 ml) adalah perlakuan terbaik terhadap kelangsungan hidup, laju pertumbuhan spesifik, panjang mutlak, dan rasio konversi pakan benih ikan nila.

PERTUMBUHAN IKAN NILA NIRWANA (Oreochromis niloticus) PADA SISTEM BIOFLOK DENGAN SUMBER KARBON EKSTERNAL DARI TEPUNG SORGUM MANIS (Sorghum bicolor)

Biofloc technology (BFT) is an alternative that can be used to provide additional feed source in addition to control and improve water quality for aquaculture. BFT can be done by adding an organic carbon source to increase the C/N ratio in  the culture media to stimulate the growth of heterotrophic bacteria. The results of the observation were that the highest specific growth rate was obtained in treatment B (5ml floc) with an average value of 2.80 grams/day. The highest average length value in treatment B (5ml floc) reached 10.26 cm. The highest survival rate was in treatment B (5ml floc) with an average value of 86.78%. The highest efficiency value was found in treatment B (5ml floc) with an average of 95.90%. Based on Duncan's further test (p<0.05) the application of biofloc with an external carbon source from sweet sorghum had a significant effect on the specific growth rate, absolute growth, survival rate and feed efficiency of tilapia fry. Keywords : Biofloc; Sweet Sorghum; Tilapia Nirwana ; Aquacuktur

UJI TERATOGENITAS EKSTRAK ETANOL 70% DAUN BUAS-BUAS (Premna serratifolia L.) TERHADAP FETUS MENCIT PUTIH (Mus musculus L.)

Salah satu bahan alam yang digunakan pada pengobatan tradisional yaitu daun buas-buas (Premna serratifolia L.). Berdasarkan penelitian sebelumnya daun buas-buas berkhasiat sebagai antidiabetes. Sebagai tanaman berkhasiat yang akan digunakan sebagai obat harus diuji keamanannya bagi kesehatan. Salah satu cara untuk mengetahui keamanan obat adalah melalui uji teratogenik. Tujuan uji teratogenik untuk mengetahui pengaruh pemberian ekstrak daun buas-buas terhadap perkembangan janin mencit. Dosis yang digunakan pada penelitian ini yaitu Kelompok normal, 600 mg/KgBB, 900 mg/KgBB, dan 1350 mg/KgBB yang diberikan pada hari ke-6 sampai ke-15. Efek yang dilihat yaitu pengamatan morfologi. Metode yang digunakan pada penelitian ini one way ANOVA dan Tukey pada rata-rata berat badan fetus. Hasilnya adalah ekstrak etanol daun buasbuas bersifat teratogenik. Ekstrak etanol daun buas-buas pada dosis 1350 mg/KgBB memberikan efek teratogenik pada fetus mencit berupa kecacatan tromboemboli dan fetus mati. Rata-rata berat badan fetus mencit secara statistik pada ektrak etanol daun buas-buas dengan dosis 600 mg/KgBB, 900 mg/KgBB, dan 1350 mg/KgBB menunjukkan adanya perbedaan bermakna. Kata kunci : Premna serratifolia L., Daun buas-buas, Teratogenik

PENGARUH PEMBERIAN SUMBER NUTRIEN BERBEDA PADA MEDIA KULTUR TERHADAP KEPADATAN POPULASI DAN LAJU PERTUMBUHAN Daphnia sp.

Penelitian ini bertujuan untuk mengetahui pengaruh pemberian sumber nutrien berbeda pada media kultur terhadap kepadatan populasi dan laju pertumbuhan Daphnia sp. Rancangan percobaan yang digunakan adalah rancangan acak lengkap non faktorial dengan tiga perlakuan dan lima kali ulangan. Perlakuan yang diteliti adalah kultur Daphnia sp. dengan pemberian air endapan dedak fermentasi pada media (A), kultur Daphnia sp. dengan pemberian air endapan kotoran ayam pada media (B) dan kultur Daphnia sp. dengan pemberian bakteri merah pada media (C). Padat tebar awal Dahpnia sp. pada masing-masing wadah adalah 100 ind/l. Masa pemeliharaan Daphnia sp. berlangsung selama 20 hari. Hasil penelitian menunjukkan bahwa pemberian sumber nutrien berbeda pada media kultur berpengaruh nyata terhadap kepadatan populasi dan laju pertumbuhan Daphnia sp. Kepadatan populasi dan laju pertumbuhan Daphnia sp. tertinggi diperoleh pada perlakuan pemberian bakteri merah dalam media kultur (C) yaitu 1271.8 ind/l dan 0,14 ind/l/hari

APLIKASI KOMBINASI TEPUNG DAUN GAMAL DAN TELUR PADA PAKAN KOMERSIAL TERHADAP KUALITAS PAKAN DAN EFISIENSI PAKAN IKAN NILA NIRWANA (Oreochromis niloticus)

The objective of the present study was to evaluate the application of a combination of gamal leaf flour and eggs in commercial feed on the feed quality and feed efficiency of nirwana tilapia (Oreochromis niloticus). The completely randomized design method was used in this study with four treatments and four replications. The treatments studied were pellet without application of combination of gamal leaf flour and eggs (P0), pellet with combination gamal leaf flour 10 % / kg of feed and eggs (P1), pellet with combination gamal leaf flour 15 % / kg of feed and eggs (P2) and pellet with combination gamal leaf flour 20 % / kg of feed and eggs (P3). Nirwana tilapia fish seed stocking density was 1 individu/l with the length of 3-4 cm. The culture period of fish farming was 50 days. The ANOVA test showed that the commercial feed with the application of a combination of gamal leaf flour and eggs gave significant effect on the feed efficiency and specific growth rate of nirwana tilapia. The highest feed protein content was obtained in the P1 treatment of 30.17%. The smell of feed produced in all treatments tended to be strong and brown. The durability of feed in water which was almost close to control was treatment P1. The best dose to increase feed quality, feed efficiency and of nirwana tilapia seed is pellet with combination gamal leaf flour 10 % / kg of feed and eggs (P1)

FEKTIVITAS PEMBERIAN KOMBINASI RAGI DAN TAURIN PADA MEDIA KULTUR TERHADAP KEPADATAN POPULASI Daphnia sp.

The objective of the present study was to evaluate the giving of baker yeast and taurine mixture in culture media on the population density of Daphnia sp.. The completely randomized design method was used in this study with four treatments and four replications. The treatments studied were the giving of chicken manure 1 g/l as control (P0), the giving of baker yeast and taurine mixture 1 g/l (P1), the giving of baker yeast and taurine mixture 2,5 g/l (P2), and the giving of baker yeast and taurine mixture 4 g/l (P3). Daphnia sp. stocking density was 100 individu/l with the size of  1,5-2 mm. The culture period of Daphnia sp. culture was 16 days. The parameters observed in this study are population density, peak population, and water quality parameters. The ANOVA test showed that the giving of yeast and taurine mix in culture media gave significant effect on the population density of Daphnia sp. Population density of  Daphnia sp. the highest was obtained in the treatment of giving 2.5 g / l of baker yeast and taurine mix in cultural media (P2) which is 1320,35 Ind / l with a peak population on the 10th day

PENGEMBANGAN ALAT PERMAINAN EDUKATIF PEROSOTAN ARITMATIKA (PETIK) UNTUK MENINGKATKAN KEMAMPUAN BERHITUNG ANAK USIA 5-6 TAHUN

This study aims to develop products in the form of appropriate and effective learning media used for learning to count, especially basic addition and subtraction for children aged 5-6 tahun years. The method used in this research is R&amp;D (research and development), namely product development in the form of an arithmetic slide educational game tool (PETIK) and data collection techniques used are observation, interviews, documentation and validation questionnaires form material experts, media and learning experts. The data analisys technique used in this study used descriptive analisys of percentages. The results of this study indicate that the arithmetic slide educational game tool (PETIK) has met the criteria for being very feasible and effective in being used in learning activities to improve the numeracy skills of children aged 5-6 years

Bioactive Compounds in Nest-Associated Bacteria Termites and Their Bioactivity as Antivibrio in Aquaculture Industry

Abstract Vibriosis is a major problem in aquaculture that causes large numbers and rapid mortality of fish. Vibriosis treatment is still limited to the same antibiotics in humans and animals. This study aims to obtain bacterial isolates from termite nests that are antivibrio and determine the profile of the antivibrio bioactive compounds. Bacterial isolates were obtained from three types of termite nests. All isolates were cultured on ISP media and then characterized and conducted bioassay tests against Vibrio alginolyticus and Vibrio harveyi. Secondary metabolite fermentation products from selected bacterial isolates were extracted using ethyl acetate and detection of bioactive compounds by Thin Layer Chromatography (TLC) method. The isolation results obtained 15 isolates, 10 Gram-positive bacteria namely isolates SR1, SR2, SR8, SR9, RE2, RE3, RE5, SE1, SE4, SE6 while the other 5 isolates are Gram-negative bacteria. Antivibrio screening obtained 7 isolates that have antivibrio activity. The strongest antivibrio activity is SE1, which was able to inhibit the growth of V. alginolyticus bacteria with an inhibition zone of 3.5 cm and V. harveyi with an inhibition zone value of 4 cm and was also positive for catalase.  TLC showed that SE1 contained peptide compounds with purple color changes after dyeing using ninhydrin