Supplementary Figures 1 - 2 from Unraveling the Role of FOXQ1 in Colorectal Cancer Metastasis

PDF file - 1023K, Supplementary Figure 1: Wound healing assay in a) SW40 and b) Colo206f cell lines. Supplementary Figure 2: Evaluation of Twist 1 knockdown on migration and invasion in stable FOXQ1 over-expressing Colo206f cell lines.</p

Minimum Active Structure of Insulin-like Peptide 5

Insulin-like peptide 5 (INSL5) is a complex two-chain peptide hormone constrained by three disulfide bonds in a pattern identical to insulin. High expression of INSL5 in the colon suggests roles in activation of colon motility and appetite control. A more recent study indicates it may have significant roles in the regulation of insulin secretion and β-cell homeostasis. This peptide thus has considerable potential for the treatment of eating disorders, obesity, and/or diabetes. However, the synthesis of INSL5 is extremely challenging either by chemical or recombinant means. The A-chain is very poorly soluble and the B-chain is highly aggregating in nature which, together, makes their postsynthesis handling and purification very difficult. Given these difficulties, we have developed a highly active INSL5 analogue that has a much simpler structure with two disulfide bonds and is thus easier to assemble compared to native INSL5. This minimized peptide represents an attractive new mimetic for investigating the functional role of INSL5