15 research outputs found
Gene & Protein expression for HPRT and ASCL1.
<p>(<b>A</b>) Gene expression for HPRT and ASCL1 in control (CTL, open bars) and HPRT knockdown (HPRTKD, closed bars) striatal STHdh cells. Bars represent mean ±SEM of duplicate PCR measurements carried out independently twice (n = 4). mRNA expression is normalized to GAPDH level (*P<0.05, t-test). (<b>B</b>) Immuno-blot and quantification of HPRT and ASCL1 protein expression in striatal STHdh cells. The quantification bar graphs are shown as means ± SEM (n = 3, *P<0.05, t-test). (<b>C</b>) Gene expression for DLX2, FOXG1 and GSX1 in control (CTL, open bars) and HPRT knockdown (HPRTKD, closed bars) Striatal cells. Bars represent mean ±SEM (n = 4) *P<0.05, t-test.</p
Schematic and summary model of HPRT-deficiency role in dysregulating striatal gene expression and signaling.
<p>HPRT-deficiency alters the purine pool; and via mechanisms that are still ill-defined, these alterations affect the gene expression of keys striatal-enriched transcription factors such as Ascl1, Bcl11b and Foxp1, notably during neurodevelopment. Later in mature neurons, these transcription factors control (directly or indirectly) the expression of several genes among them, <i>Darpp-32</i> and <i>Bdnf</i>, whose the dysregulation affects striatal neurons signaling and neurotransmission, thus contributing to LNS neurological phenotype.</p
Gene and Protein Expression for BDNF and TRKB.
<p>(<b>A</b>) Gene expression for <i>Bdnf</i> and <i>Trkb</i> in control (CTL, open bars) and HPRT knockdown (HPRTKD, closed bars) striatal STHdh cells. Bars represent mean ±SEM (n = 4). mRNA expression is normalized to GAPDH RNA level (*P<0.05, t-test). (<b>B</b>) Immuno-blot and quantification of BDNF and TRKB protein expression in striatal cells. The quantification bar graphs are shown as means ± SEM (n = 3, *P<0.05, t-test). (<b>C</b>) Gene expression for <i>Bdnf</i> and <i>Trkb</i> in the striatum of wild-type (WT, open bars) and HPRT knockout (HPRTKO, closed bars) mice. Bars represent mean ±SEM of duplicate PCR measurements (n = 4). mRNA expression is normalized to GAPDH mRNA level (*P<0.05, t-test). (<b>D</b>) Immuno-blot and quantification of BDNF and TrkB protein expression in the striatum of WT and HPRTKO mice. The quantification bar graphs are shown as means ± SEM (n = 3, *P<0.05, t-test). (<b>E</b>) (Y705/706) TrkB phosphoprotein expression in response to BDNF treatment (10 ng/ml). Immuno-blot and quantification of Y705/706) TrkB phosphoprotein in control and HPRT-deficient striatal cells, the quantification bar graphs are shown as means ± SEM (n = 6, *P<0.05).</p
Striatal Neurodevelopment Is Dysregulated in Purine Metabolism Deficiency and Impacts DARPP-32, BDNF/TrkB Expression and Signaling: New Insights on the Molecular and Cellular Basis of Lesch-Nyhan Syndrome
<div><p>Lesch-Nyhan Syndrome (LNS) is a neurodevelopmental disorder caused by mutations in the gene encoding the purine metabolic enzyme hypoxanthine-guanine phosphoribosyltransferase (HPRT). This syndrome is characterized by an array of severe neurological impairments that in part originate from striatal dysfunctions. However, the molecular and cellular mechanisms underlying these dysfunctions remain largely unidentified. In this report, we demonstrate that HPRT-deficiency causes dysregulated expression of key genes essential for striatal patterning, most notably the striatally-enriched transcription factor B-cell leukemia 11b (Bcl11b). The data also reveal that the down-regulated expression of Bcl11b in HPRT-deficient immortalized mouse striatal (STH<i>dh</i>) neural stem cells is accompanied by aberrant expression of some of its transcriptional partners and other striatally-enriched genes, including the gene encoding dopamine- and cAMP-regulated phosphoprotein 32, (DARPP-32). Furthermore, we demonstrate that components of the BDNF/TrkB signaling, a known activator of DARPP-32 striatal expression and effector of Bcl11b transcriptional activation are markedly increased in HPRT-deficient cells and in the striatum of HPRT knockout mouse. Consequently, the HPRT-deficient cells display superior protection against reactive oxygen species (ROS)-mediated cell death upon exposure to hydrogen peroxide. These findings suggest that the purine metabolic defect caused by HPRT-deficiency, while it may provide neuroprotection to striatal neurons, affects key genes and signaling pathways that may underlie the neuropathogenesis of LNS.</p></div
Effects of hydrogen peroxide-mediated cellular toxicity in HPRT-deficient striatal cells:
<p>(<b>A</b>) (Y705/706) TrkB phosphoprotein expression in response to hydrogen peroxide H<sub>2</sub>O<sub>2</sub> treatment (100 µM). Immuno-blot and quantification of Y705/706 TrkB phosphoprotein in control and HPRT-deficient striatal STHdh cells. The quantification bar graphs are shown as means ± SEM (n = 6, *P<0.05). (<b>B</b>) Enhanced neuroprotective effects against reactive oxygen species (ROS)-mediated cell death in HPRT-deficient striatal cells. Control (CTL) and HPRT-knock-down cells (HPRTKD) were treated with hydrogen peroxide H<sub>2</sub>O<sub>2</sub> for 4 hours and then exposed to Sytox green for 10 min. Figure shows microscopy images of DAPI staining and green fluorescence which is a measure of the overall cell death level. There is reduced green fluorescence in HPRT-deficient cells relative to control after stimulation with H<sub>2</sub>O<sub>2</sub>. (Bar scale, 100 µm). This is confirmed by the quantification of the number of Sytox green fluorescent cells as illustrated in the appended graph. Error bars represent mean ± SEM of duplicate measurements of two independent experiments (n = 4). The asterisks (*) represent statistical significance between H<sub>2</sub>O<sub>2</sub> treated cells (p<0.05, t-test).</p
Gene and Protein Expression for Bcll11b.
<p>(<b>A</b>) Gene expression for Bcl11b in control striatal STHdh cells (CTL, open bars) and HPRT-knockdown (HPRTKD, closed bars). Bars represent mean ±SEM (n = 4). mRNA expression is normalized to GAPDH RNA level (*P<0.05, t-test). (<b>B</b>) Immuno-blot and quantification of Bcl11b protein expression in striatal cells. The quantification bar graphs are shown as means ± SEM (n = 3, *P<0.05, t-test). (<b>C</b>) Gene expression of Actn2, Arpp19, Foxp1, Ngef, Pde10a, and Rgs9 in control (CTL, open bars) and HPRT knockdown (HPRTKD, closed bars) striatal STHdh cells. Bars represent mean ±SEM (n = 4). *P<0.05, t-test. (<b>D</b>) Gene expression for Bcl11b in the striatum of wild-type (WT, open bars) and HPRT knockout (HPRTKO, closed bars) mice. Bars represent mean ±SEM (n = 4). mRNA expression is normalized to GAPDH RNA level (*P<0.05, t-test). (<b>E</b>) Immuno-blot and quantification of Bcl11b protein expression in the striatum of WT and HPRTKO mice. The quantification bar graphs are shown as means ± SEM (n = 3, *P<0.05, t-test). (<b>F</b>) Gene expression for Actn2, Arpp19, Foxp1, Ngef, Pde10a, and Rgs9 in control (Wild-type, open bars) and HPRT knockout mice (HPRTKO, closed bars) striatum. Bars represent mean ±SEM (n = 4). *P<0.05, t-test.</p
Bcl11b and <i>Ppp1r1b</i>/DARPP-32 in human fibroblasts.
<p>(<b>A</b>) <i>Bcl11b</i> expression in individual fibroblast samples from five control (normal) subjects (C1–C5) and from five patients with LNS (L1–L5). Δct values are normalized to control mRNA (GAPDH). (<b>B</b>) Normalized <i>Bcl11b</i> expression in controls (CTL) and LNS patients. (<b>C</b>) <i>Ppp1r1b/</i>DARPP-32 expression in individual fibroblast samples from five control (normal) subjects (C1–C5) and from five patients with LNS (L1–L5). Δct values are normalized to control mRNA (GAPDH). (<b>D</b>) Normalized <i>Ppp1r1b</i>/DARPP-32 expression in controls (CTL) and LNS patients (*<i>P<</i>0.05, <i>t</i>-test).</p
Restoration of Bcl11b expression in HPRT-deficient striatal cells:
<p>(<b>A</b>) Immuno-blot and quantification of Bcl11b expression in control (CTL) and HPRT-deficient striatal cells transfected with plasmid encoding Bcl11b (Bcl11b) and GFP (GFP). The quantification bar graphs are shown means± SEM (n = 3, *P<0.05, ANOVA, Tukey post-hoc test). (<b>B</b>) Immuno-blot and quantification of Y705/706 TrkB after H<sub>2</sub>O<sub>2</sub> exposure for control (CTL), HPRT-deficient cells transfected with Bcl11b (Bcl11b) and GFP (GFP). The quantification bar graphs are shown as means ± SEM (n = 4, *P<0.05, ANOVA, Tukey post-hoc test). (<b>C</b>) quantification of the number of Sytox green fluorescent cells upon treatment of control (CTL) and HPRT-deficient cells transfected with Bcl11b (BCL11b) and GFP (GFP) plasmid. Data show that the rescue of Bcl11b expression in HPRT-deficient cells does not lead to added protection from cell death triggered by H<sub>2</sub>O<sub>2</sub> (ns =  non significant, *p<0.05, ANOVA). (<b>D</b>) Gene expression of DARPP-32 in control (CTL), GFP and Bcl11b (rescued) transfected cells. Data show that the rescued expression of Bcl11b in HPRT-deficient cells leads to restoration of DARPP-32 expression in level similar to control, the data are shown as means ± SEM ((ns =  non significant, n = 4, *P<0.05, ANOVA, Tukey post-hoc test). (<b>E</b>) Gene expression for Actn2, Arpp19, Foxp1, Ngef, Pde10a, and Rgs9 in control (CTL, open bars), GFP (GFP, closed bars) and Bcl11b (Bcl11b, grey bars) transfected striatal cells. Bars represent mean ±SEM (n = 4). *P<0.05, ANOVA.</p
Gene and Protein Expression for <i>Ppp1r1b</i>/DARPP-32.
<p>(<b>A</b>) Gene expression for <i>Ppp1r1b</i>/DARPP-32 in control striatal STHdh cells (CTL, open bars) and HPRT-knockdown (HPRTKD, closed bars). Bars represent mean ±SEM (n = 4). mRNA expression is normalized to GAPDH mRNA level (*P<0.05, t-test). (<b>B</b>) Immuno-blot and quantification of DARPP-32 protein expression in striatal cells. The quantification bar graphs are shown as means ± SEM (n = 3, *P<0.05, t-test). (<b>C</b>) Gene expression for <i>Ppp1r1b</i>/DARPP-32 in the striatum of wild-type (WT, open bars) and HPRT knockout (HPRTKO, closed bars) mice. Bars represent mean ±SEM (n = 4). mRNA expression is normalized to GAPDH RNA level (*P<0.05, t-test). (<b>D</b>) Immuno-blot and quantification of DARPP-32 protein expression in the striatum of WT and HPRTKO mice. The quantification bar graphs are shown as means ± SEM (n = 3, *P<0.05, t-test). (<b>E</b>) DARPP-32 phosphoprotein (Thr34) expression in response to D1R (SKF393298) agonist treatment (50 µM). Immuno-blot and quantification of Thr34-DARPP-32 in control and HPRT-deficient striatal cells, the quantification bar graphs are shown as means ± SEM (n = 6, *P<0.05). (<b>F</b>) Thr34-DARPP-32 phosphoprotein expression in striatal tissue of WT (open bar) and HPRTKO (closed bar) mice (n = 3, *P<0.05).</p
Cardiac Glycosides Activate the Tumor Suppressor and Viral Restriction Factor Promyelocytic Leukemia Protein (PML)
<div><p>Cardiac glycosides (CGs), inhibitors of Na<sup>+</sup>/K<sup>+</sup>-ATPase (NKA), used clinically to treat heart failure, have garnered recent attention as potential anti-cancer and anti-viral agents. A high-throughput phenotypic screen designed to identify modulators of promyelocytic leukemia protein (PML) nuclear body (NB) formation revealed the CG gitoxigenin as a potent activator of PML. We demonstrate that multiple structurally distinct CGs activate the formation of PML NBs and induce PML protein SUMOylation in an NKA-dependent fashion. CG effects on PML occur at the post-transcriptional level, mechanistically distinct from previously described PML activators and are mediated through signaling events downstream of NKA. Curiously, genomic deletion of PML in human cancer cells failed to abrogate the cytotoxic effects of CGs and other apoptotic stimuli such as ceramide and arsenic trioxide that were previously shown to function through PML in mice. These findings suggest that alternative pathways can compensate for PML loss to mediate apoptosis in response to CGs and other apoptotic stimuli.</p></div