26 research outputs found
Quantification of expression levels of transgenes by qPCR.
<p>Five different Arabidopsis transformants expressing four different spruce genes were created using <i>Agrobacterium</i>-mediated DNA transfer. Estimated expression levels were log2 transformed. See <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0060110#s2" target="_blank">Materials & Methods</a> for details about calculation of expression levels. Arabidopsis <i>α-tubulin</i> was used as endogenous control. Error bars indicate SE values.</p
Flowering time was altered for some of the Arabidopsis transgenic lines.
<p>Selected lines expressing (A) <i>PaCCA1</i>, (B) <i>PaGI</i>, (C) <i>PaGI</i> in the <i>gi2</i> mutant and (D) <i>PaZTL</i> were grown in LD (18 h light/6 h dark or 16 h light/8 h dark) and SD (9 h light/15 h dark) and flowering time was assessed as number of rosette leaves when the first flower bud was visible in the rosette. Pictures of representative plants grown in LD for 46 days were taken for (E) <i>PaCCA1</i>, (G) <i>PaGI</i> in the <i>gi2</i> mutant and (H) <i>PaZTL</i>. In <i>PaCCA1-ox</i> the petioles were longer than the wt (F). Error bars indicate SE values.</p
Domain structure of CCA1, ZTL, PRR1 and GI.
<p>Protein domain structure in Arabidopsis (<i>A.t.</i>) and Norway spruce (<i>P.a.</i>). The amino acid similarity in the different domains is stated below each domain and is given as identical amino acids/similar amino acids in percentage. The similarity between the two proteins is given in the same way as for the domains. The domain position and similarity percentage were retrieved using BLAST alignment tool at the NCBI homepage and the whole protein similarity was calculated using Emboss Needle – Pairwise sequence alignment.</p
Circadian period of leaf movement in Arabidopsis lines expressing spruce clock genes.
<p>Weighted mean circadian periods were estimated as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0060110#s2" target="_blank">Materials and Methods</a>. SEM, standard error of the mean; RAE, relative amplitude error; n, number of rhythmic plants.</p>*<p>No rhythmic plants were observed for <i>PaCCA1-ox</i> lines.</p
Statistical tests for altered sensitivity to red light among Arabidopsis lines expressing spruce clock genes.
<p>Transgenic lines were compared against the corresponding Arabidopsis lines without transgene.</p>*<p><i>P</i>-value calculated from the interaction term Fluency rate x Line in a linear model including Line and Fluency rate, see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0060110#s2" target="_blank">materials and methods</a> for details.</p>**<p>Adjusted R<sup>2</sup> (coefficient of determination) indicating model fit.</p>***<p>Hypo and Hyper indicate that ectopic expressor lines were hypo- or hypersensitive to red light as compared to untransformed lines. Parentheses mark non-significant effects.</p
Seppa et al_dryad data
Genotypes of individual workers collected in the field + spatial coordinates of their nests. See the ReadMe fil